Multi-omics analysis of hexaploid triticale that show molecular responses to salt stress during seed germination
The development of a salt-tolerant hexaploid triticale cultivar offers an economical and efficient solution for utilizing marginal land. Understanding how hexaploid triticales respond to salt stress is essential if this goal is to be achieved. A genome-wide association study (GWAS), along with trans...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1529961/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832592428439175168 |
|---|---|
| author | Dongxia Wang Jiedong Li Shiming Li Jiongjie Fu Baolong Liu Baolong Liu Baolong Liu Dong Cao Dong Cao Dong Cao |
| author_facet | Dongxia Wang Jiedong Li Shiming Li Jiongjie Fu Baolong Liu Baolong Liu Baolong Liu Dong Cao Dong Cao Dong Cao |
| author_sort | Dongxia Wang |
| collection | DOAJ |
| description | The development of a salt-tolerant hexaploid triticale cultivar offers an economical and efficient solution for utilizing marginal land. Understanding how hexaploid triticales respond to salt stress is essential if this goal is to be achieved. A genome-wide association study (GWAS), along with transcriptome and proteome analyses, were used in the present study to determine the molecular responses to salt stress in hexaploid triticale. In total, 81 marker-trait associations for 10 salt-tolerance traits were identified in 153 hexaploid triticale accessions, explaining 0.71% to 56.98% of the phenotypic variation, and 54 GWAS-associated genes were uncovered. A total of 67, 88, and 688 differential expression genes were co-expressed at both the transcriptomic and proteomic levels after 4, 12, and 18 h of salt stress, respectively. Among these differentially expressed genes, six appeared in the coincident expression trends for both the transcriptomic and proteomic levels at the seed germination stage. A total of nine common KEGG pathways were enriched at both the transcriptomic and proteomic levels at 4, 12, and 18 h. After integrating GWAS-target genes with transcriptomics and proteomics approaches that the candidate gene late embryogenesis abundant protein 14 (LEA14) was up-regulated at the transcriptomic and proteomic levels. LEA14 contained important stress-responsive cis-acting regulatory elements that could be dynamically regulated by the binding of transcription factors (TFs). This suggested that LEA14 was a key gene associated with salt tolerance in hexaploid triticale and could respond quickly to salt stress. This study improved understanding about the potential molecular mechanisms associated with hexaploid triticale salt tolerance and contributed to the breeding of salt-tolerant germplasms and the utilization of saline soils. |
| format | Article |
| id | doaj-art-add4ccf523b94682894e6f701c306b2d |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-add4ccf523b94682894e6f701c306b2d2025-01-21T08:36:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15299611529961Multi-omics analysis of hexaploid triticale that show molecular responses to salt stress during seed germinationDongxia Wang0Jiedong Li1Shiming Li2Jiongjie Fu3Baolong Liu4Baolong Liu5Baolong Liu6Dong Cao7Dong Cao8Dong Cao9Department of Agriculture and Forestry, College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, ChinaDepartment of Agriculture and Forestry, College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, ChinaQinghai Province Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaDepartment of Agriculture and Forestry, College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, ChinaQinghai Province Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaQinghai Province Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaThe development of a salt-tolerant hexaploid triticale cultivar offers an economical and efficient solution for utilizing marginal land. Understanding how hexaploid triticales respond to salt stress is essential if this goal is to be achieved. A genome-wide association study (GWAS), along with transcriptome and proteome analyses, were used in the present study to determine the molecular responses to salt stress in hexaploid triticale. In total, 81 marker-trait associations for 10 salt-tolerance traits were identified in 153 hexaploid triticale accessions, explaining 0.71% to 56.98% of the phenotypic variation, and 54 GWAS-associated genes were uncovered. A total of 67, 88, and 688 differential expression genes were co-expressed at both the transcriptomic and proteomic levels after 4, 12, and 18 h of salt stress, respectively. Among these differentially expressed genes, six appeared in the coincident expression trends for both the transcriptomic and proteomic levels at the seed germination stage. A total of nine common KEGG pathways were enriched at both the transcriptomic and proteomic levels at 4, 12, and 18 h. After integrating GWAS-target genes with transcriptomics and proteomics approaches that the candidate gene late embryogenesis abundant protein 14 (LEA14) was up-regulated at the transcriptomic and proteomic levels. LEA14 contained important stress-responsive cis-acting regulatory elements that could be dynamically regulated by the binding of transcription factors (TFs). This suggested that LEA14 was a key gene associated with salt tolerance in hexaploid triticale and could respond quickly to salt stress. This study improved understanding about the potential molecular mechanisms associated with hexaploid triticale salt tolerance and contributed to the breeding of salt-tolerant germplasms and the utilization of saline soils.https://www.frontiersin.org/articles/10.3389/fpls.2024.1529961/fullhexaploid triticalesalt tolerancegermination stagemulti-omicsmolecular response |
| spellingShingle | Dongxia Wang Jiedong Li Shiming Li Jiongjie Fu Baolong Liu Baolong Liu Baolong Liu Dong Cao Dong Cao Dong Cao Multi-omics analysis of hexaploid triticale that show molecular responses to salt stress during seed germination Frontiers in Plant Science hexaploid triticale salt tolerance germination stage multi-omics molecular response |
| title | Multi-omics analysis of hexaploid triticale that show molecular responses to salt stress during seed germination |
| title_full | Multi-omics analysis of hexaploid triticale that show molecular responses to salt stress during seed germination |
| title_fullStr | Multi-omics analysis of hexaploid triticale that show molecular responses to salt stress during seed germination |
| title_full_unstemmed | Multi-omics analysis of hexaploid triticale that show molecular responses to salt stress during seed germination |
| title_short | Multi-omics analysis of hexaploid triticale that show molecular responses to salt stress during seed germination |
| title_sort | multi omics analysis of hexaploid triticale that show molecular responses to salt stress during seed germination |
| topic | hexaploid triticale salt tolerance germination stage multi-omics molecular response |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1529961/full |
| work_keys_str_mv | AT dongxiawang multiomicsanalysisofhexaploidtriticalethatshowmolecularresponsestosaltstressduringseedgermination AT jiedongli multiomicsanalysisofhexaploidtriticalethatshowmolecularresponsestosaltstressduringseedgermination AT shimingli multiomicsanalysisofhexaploidtriticalethatshowmolecularresponsestosaltstressduringseedgermination AT jiongjiefu multiomicsanalysisofhexaploidtriticalethatshowmolecularresponsestosaltstressduringseedgermination AT baolongliu multiomicsanalysisofhexaploidtriticalethatshowmolecularresponsestosaltstressduringseedgermination AT baolongliu multiomicsanalysisofhexaploidtriticalethatshowmolecularresponsestosaltstressduringseedgermination AT baolongliu multiomicsanalysisofhexaploidtriticalethatshowmolecularresponsestosaltstressduringseedgermination AT dongcao multiomicsanalysisofhexaploidtriticalethatshowmolecularresponsestosaltstressduringseedgermination AT dongcao multiomicsanalysisofhexaploidtriticalethatshowmolecularresponsestosaltstressduringseedgermination AT dongcao multiomicsanalysisofhexaploidtriticalethatshowmolecularresponsestosaltstressduringseedgermination |