The Impact of Maturation Medium Supplemented with PMSG and HCG Hormones on In vitro Maturation of Ovine Oocytes
This investigation aimed to assess the dose-dependent impact of the pregnant mare serum gonadotropin (PMSG) on the in vitro maturation of sheep oocytes and the effect of combining the best dose with the human chorionic gonadotropin (hCG), which is a cost-effective substitute for FSH and LH hormones....
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Egyptian Society for Animal Management
2025-01-01
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| Series: | Journal of Applied Veterinary Sciences |
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| Online Access: | https://javs.journals.ekb.eg/article_397380_8fd9289f7f1def264365228b4c8c2caa.pdf |
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| author | Fatma Baiomy Ahmed Kamel Abdelhadi Hussein Sabry Hassanin |
| author_facet | Fatma Baiomy Ahmed Kamel Abdelhadi Hussein Sabry Hassanin |
| author_sort | Fatma Baiomy |
| collection | DOAJ |
| description | This investigation aimed to assess the dose-dependent impact of the pregnant mare serum gonadotropin (PMSG) on the in vitro maturation of sheep oocytes and the effect of combining the best dose with the human chorionic gonadotropin (hCG), which is a cost-effective substitute for FSH and LH hormones. The study was planned as two experiments that included 1134 oocytes to study the impact of varying concentrations (0, 10, 20, and 40 IU/mL) of PMSG addition (1st experiment) as well as the influence of hCG, PMSG, or their combination (2nd experiment) on the ovine’s oocyte in vitro. The experimental groups of the 2nd experiment were control (G1), 20 IU/mL hCG (G2), 20 IU/mL PMSG (G3), and 20 IU/mL PMSG plus 20 IU/mL hCG (G4). The first experiment demonstrated that the polar body (PB) rate was increased (P < 0.01) by all treated groups, being 13.33, 17.76, and 15.70 in G2, G3, and G4 versus 9.87% in G1, respectively. The mitochondrial fluorescent intensity recorded 7.61, 11.92, 12.43, and 12.35, while the lipid fluorescent intensity was 9.88, 29.98, 40.05, and 25.66 for G1, G2, G3, and G4, respectively, being higher (P < 0.05) in treatment groups than in the control one. The results of the 2nd experiment showed that the PB extrusion was higher (P < 0.01) in G2, G3, and G4 than in G1 (12.60, 14.91, and 14.75 vs. 6.92%, respectively). The mitochondrial fluorescent intensity recorded 8.82, 12.30, 17.58, and 13.36 for G1, G2, G3, and G4, respectively. The lipid fluorescent intensity was 10.16, 6.98, 13.83, and 49.68 for G1, G2, G3, and G4, respectively. In conclusion, the addition of PMSG and hCG to sheep oocyte maturation media improved the oocyte maturation. |
| format | Article |
| id | doaj-art-adc767b35c884d7cbbda551c204918f2 |
| institution | Kabale University |
| issn | 1687-4072 2090-3308 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Egyptian Society for Animal Management |
| record_format | Article |
| series | Journal of Applied Veterinary Sciences |
| spelling | doaj-art-adc767b35c884d7cbbda551c204918f22025-01-01T19:48:26ZengEgyptian Society for Animal ManagementJournal of Applied Veterinary Sciences1687-40722090-33082025-01-01101819110.21608/javs.2024.335751.1464397380The Impact of Maturation Medium Supplemented with PMSG and HCG Hormones on In vitro Maturation of Ovine OocytesFatma Baiomy0Ahmed Kamel1Abdelhadi Hussein2Sabry Hassanin3Animal Production Department, Faculty of Agriculture, Ain Shams University, Cairo, EgyptDepartment of Animal and Poultry Physiology, Animal and Poultry Production Division, Desert Research Center (DRC), Cairo, EgyptAnimal Production Department, Faculty of Agriculture, Ain Shams University, Cairo, EgyptAnimal Production Department, Faculty of Agriculture, Ain Shams University, Cairo, EgyptThis investigation aimed to assess the dose-dependent impact of the pregnant mare serum gonadotropin (PMSG) on the in vitro maturation of sheep oocytes and the effect of combining the best dose with the human chorionic gonadotropin (hCG), which is a cost-effective substitute for FSH and LH hormones. The study was planned as two experiments that included 1134 oocytes to study the impact of varying concentrations (0, 10, 20, and 40 IU/mL) of PMSG addition (1st experiment) as well as the influence of hCG, PMSG, or their combination (2nd experiment) on the ovine’s oocyte in vitro. The experimental groups of the 2nd experiment were control (G1), 20 IU/mL hCG (G2), 20 IU/mL PMSG (G3), and 20 IU/mL PMSG plus 20 IU/mL hCG (G4). The first experiment demonstrated that the polar body (PB) rate was increased (P < 0.01) by all treated groups, being 13.33, 17.76, and 15.70 in G2, G3, and G4 versus 9.87% in G1, respectively. The mitochondrial fluorescent intensity recorded 7.61, 11.92, 12.43, and 12.35, while the lipid fluorescent intensity was 9.88, 29.98, 40.05, and 25.66 for G1, G2, G3, and G4, respectively, being higher (P < 0.05) in treatment groups than in the control one. The results of the 2nd experiment showed that the PB extrusion was higher (P < 0.01) in G2, G3, and G4 than in G1 (12.60, 14.91, and 14.75 vs. 6.92%, respectively). The mitochondrial fluorescent intensity recorded 8.82, 12.30, 17.58, and 13.36 for G1, G2, G3, and G4, respectively. The lipid fluorescent intensity was 10.16, 6.98, 13.83, and 49.68 for G1, G2, G3, and G4, respectively. In conclusion, the addition of PMSG and hCG to sheep oocyte maturation media improved the oocyte maturation.https://javs.journals.ekb.eg/article_397380_8fd9289f7f1def264365228b4c8c2caa.pdfhcgivmoocytespmsgsheep |
| spellingShingle | Fatma Baiomy Ahmed Kamel Abdelhadi Hussein Sabry Hassanin The Impact of Maturation Medium Supplemented with PMSG and HCG Hormones on In vitro Maturation of Ovine Oocytes Journal of Applied Veterinary Sciences hcg ivm oocytes pmsg sheep |
| title | The Impact of Maturation Medium Supplemented with PMSG and HCG Hormones on In vitro Maturation of Ovine Oocytes |
| title_full | The Impact of Maturation Medium Supplemented with PMSG and HCG Hormones on In vitro Maturation of Ovine Oocytes |
| title_fullStr | The Impact of Maturation Medium Supplemented with PMSG and HCG Hormones on In vitro Maturation of Ovine Oocytes |
| title_full_unstemmed | The Impact of Maturation Medium Supplemented with PMSG and HCG Hormones on In vitro Maturation of Ovine Oocytes |
| title_short | The Impact of Maturation Medium Supplemented with PMSG and HCG Hormones on In vitro Maturation of Ovine Oocytes |
| title_sort | impact of maturation medium supplemented with pmsg and hcg hormones on in vitro maturation of ovine oocytes |
| topic | hcg ivm oocytes pmsg sheep |
| url | https://javs.journals.ekb.eg/article_397380_8fd9289f7f1def264365228b4c8c2caa.pdf |
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