Intestinal Carriage of Two Distinct <i>stx<sub>2f</sub></i>-Carrying <i>Escherichia coli</i> Strains by a Child with Uncomplicated Diarrhea
Two distinct <i>stx<sub>2f</sub></i>-carrying <i>Escherichia coli</i> (<i>E. coli</i>) strains, isolated from a child with uncomplicated diarrhea fifteen weeks apart, were characterized by combining short- and long-read sequencing to compare their gene...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/13/11/1002 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850227063665983488 |
|---|---|
| author | Florence Crombé Angela H. A. M. van Hoek Heleen Nailis Frédéric Auvray Toon Janssen Denis Piérard |
| author_facet | Florence Crombé Angela H. A. M. van Hoek Heleen Nailis Frédéric Auvray Toon Janssen Denis Piérard |
| author_sort | Florence Crombé |
| collection | DOAJ |
| description | Two distinct <i>stx<sub>2f</sub></i>-carrying <i>Escherichia coli</i> (<i>E. coli</i>) strains, isolated from a child with uncomplicated diarrhea fifteen weeks apart, were characterized by combining short- and long-read sequencing to compare their genetic relatedness. One strain was characterized as Shiga toxin-producing <i>E. coli</i> (STEC)/typical enteropathogenic <i>E. coli</i> (tEPEC) O63:H6 with a repertoire of virulence genes including <i>stx<sub>2f</sub></i>, <i>eae</i> (α2-subtype), <i>cdt</i>, and <i>bfpA</i>. The other STEC with serotype O157:H16, reported for the first time as <i>stx<sub>2f</sub></i>-carrying <i>Escherichia coli</i> in this study, possessed, in addition, <i>eae</i> (ε-subtype) and <i>cdt</i>, amongst other virulence-related genes. BLAST comparison showed that the <i>stx<sub>2f</sub></i>-harboring prophage sequences of both strains were highly homologous (99.6% identity and 96.1% coverage). These results were corroborated by core Stx2f phage Multilocus Sequence Typing (cpMLST) as the <i>stx<sub>2f</sub></i>-harboring prophages of both isolates clustered together when compared to those of 167 other human <i>stx<sub>2f</sub></i>-carrying <i>Escherichia coli</i>. Overall, the <i>stx<sub>2f</sub></i>-harboring prophages of the two distinct <i>E. coli</i> strains isolated from the present case were highly similar, suggesting that the <i>stx<sub>2f</sub></i>-harboring phage might have been transferred from the STEC/tEPEC O63:H6 strain to the atypical EPEC (aEPEC) O157:H16 strain in the gut of the child. |
| format | Article |
| id | doaj-art-adc57f43574a40b1abf73a2f05ca96a2 |
| institution | OA Journals |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-adc57f43574a40b1abf73a2f05ca96a22025-08-20T02:04:55ZengMDPI AGPathogens2076-08172024-11-011311100210.3390/pathogens13111002Intestinal Carriage of Two Distinct <i>stx<sub>2f</sub></i>-Carrying <i>Escherichia coli</i> Strains by a Child with Uncomplicated DiarrheaFlorence Crombé0Angela H. A. M. van Hoek1Heleen Nailis2Frédéric Auvray3Toon Janssen4Denis Piérard5Department Clinical Biology, Laboratory of Microbiology and Infection Control, Belgian National Reference Centre for STEC/VTEC, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), 1090 Brussels, BelgiumCentre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), 3721 BA Bilthoven, The NetherlandsDepartment of Laboratory Medicine, AZ Turnhout, 2300 Turnhout, AZ Herentals, 2200 Herentals, Heilig Hart Mol, 2400 Mol, BelgiumIRSD, Faculté des Sciences, Université de Toulouse, INSERM, INRAE, ENVT, UPS, 31000 Toulouse, FranceBrussels Interuniversity Genomics High Throughput Core (BRIGHTcore) Platform, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), 1090 Brussels, BelgiumDepartment Clinical Biology, Laboratory of Microbiology and Infection Control, Belgian National Reference Centre for STEC/VTEC, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), 1090 Brussels, BelgiumTwo distinct <i>stx<sub>2f</sub></i>-carrying <i>Escherichia coli</i> (<i>E. coli</i>) strains, isolated from a child with uncomplicated diarrhea fifteen weeks apart, were characterized by combining short- and long-read sequencing to compare their genetic relatedness. One strain was characterized as Shiga toxin-producing <i>E. coli</i> (STEC)/typical enteropathogenic <i>E. coli</i> (tEPEC) O63:H6 with a repertoire of virulence genes including <i>stx<sub>2f</sub></i>, <i>eae</i> (α2-subtype), <i>cdt</i>, and <i>bfpA</i>. The other STEC with serotype O157:H16, reported for the first time as <i>stx<sub>2f</sub></i>-carrying <i>Escherichia coli</i> in this study, possessed, in addition, <i>eae</i> (ε-subtype) and <i>cdt</i>, amongst other virulence-related genes. BLAST comparison showed that the <i>stx<sub>2f</sub></i>-harboring prophage sequences of both strains were highly homologous (99.6% identity and 96.1% coverage). These results were corroborated by core Stx2f phage Multilocus Sequence Typing (cpMLST) as the <i>stx<sub>2f</sub></i>-harboring prophages of both isolates clustered together when compared to those of 167 other human <i>stx<sub>2f</sub></i>-carrying <i>Escherichia coli</i>. Overall, the <i>stx<sub>2f</sub></i>-harboring prophages of the two distinct <i>E. coli</i> strains isolated from the present case were highly similar, suggesting that the <i>stx<sub>2f</sub></i>-harboring phage might have been transferred from the STEC/tEPEC O63:H6 strain to the atypical EPEC (aEPEC) O157:H16 strain in the gut of the child.https://www.mdpi.com/2076-0817/13/11/1002Shiga toxin-producing <i>Escherichia coli</i> (STEC)Stx2f<i>Escherichia coli</i> O157:H16whole-genome sequencing |
| spellingShingle | Florence Crombé Angela H. A. M. van Hoek Heleen Nailis Frédéric Auvray Toon Janssen Denis Piérard Intestinal Carriage of Two Distinct <i>stx<sub>2f</sub></i>-Carrying <i>Escherichia coli</i> Strains by a Child with Uncomplicated Diarrhea Pathogens Shiga toxin-producing <i>Escherichia coli</i> (STEC) Stx2f <i>Escherichia coli</i> O157:H16 whole-genome sequencing |
| title | Intestinal Carriage of Two Distinct <i>stx<sub>2f</sub></i>-Carrying <i>Escherichia coli</i> Strains by a Child with Uncomplicated Diarrhea |
| title_full | Intestinal Carriage of Two Distinct <i>stx<sub>2f</sub></i>-Carrying <i>Escherichia coli</i> Strains by a Child with Uncomplicated Diarrhea |
| title_fullStr | Intestinal Carriage of Two Distinct <i>stx<sub>2f</sub></i>-Carrying <i>Escherichia coli</i> Strains by a Child with Uncomplicated Diarrhea |
| title_full_unstemmed | Intestinal Carriage of Two Distinct <i>stx<sub>2f</sub></i>-Carrying <i>Escherichia coli</i> Strains by a Child with Uncomplicated Diarrhea |
| title_short | Intestinal Carriage of Two Distinct <i>stx<sub>2f</sub></i>-Carrying <i>Escherichia coli</i> Strains by a Child with Uncomplicated Diarrhea |
| title_sort | intestinal carriage of two distinct i stx sub 2f sub i carrying i escherichia coli i strains by a child with uncomplicated diarrhea |
| topic | Shiga toxin-producing <i>Escherichia coli</i> (STEC) Stx2f <i>Escherichia coli</i> O157:H16 whole-genome sequencing |
| url | https://www.mdpi.com/2076-0817/13/11/1002 |
| work_keys_str_mv | AT florencecrombe intestinalcarriageoftwodistinctistxsub2fsubicarryingiescherichiacoliistrainsbyachildwithuncomplicateddiarrhea AT angelahamvanhoek intestinalcarriageoftwodistinctistxsub2fsubicarryingiescherichiacoliistrainsbyachildwithuncomplicateddiarrhea AT heleennailis intestinalcarriageoftwodistinctistxsub2fsubicarryingiescherichiacoliistrainsbyachildwithuncomplicateddiarrhea AT fredericauvray intestinalcarriageoftwodistinctistxsub2fsubicarryingiescherichiacoliistrainsbyachildwithuncomplicateddiarrhea AT toonjanssen intestinalcarriageoftwodistinctistxsub2fsubicarryingiescherichiacoliistrainsbyachildwithuncomplicateddiarrhea AT denispierard intestinalcarriageoftwodistinctistxsub2fsubicarryingiescherichiacoliistrainsbyachildwithuncomplicateddiarrhea |