Accuracy and acceptability of self-sampling HPV testing in cervical cancer screening: a population-based study in rural Yunnan, China
Abstract To evaluate the accuracy and acceptability of self-sampling samples for HPV testing for cervical cancer screening in rural Yunnan of China. In 2022, 3000 women aged 17–69 were recruited and provided self-sampling vaginal samples alongside provider-sampling samples for HPV DNA and E6/E7 mRNA...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-09292-5 |
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| author | Rufei Duan Hongying Yang Xiuwei Zhai Jing Yu Chen Lu Wenmin Yang Tingfeng Long Yan Dao Gaofeng Li Yongchun Zhou Hongyi Zheng Fanghui Zhao Yong-Tang Zheng Yunchao Huang |
| author_facet | Rufei Duan Hongying Yang Xiuwei Zhai Jing Yu Chen Lu Wenmin Yang Tingfeng Long Yan Dao Gaofeng Li Yongchun Zhou Hongyi Zheng Fanghui Zhao Yong-Tang Zheng Yunchao Huang |
| author_sort | Rufei Duan |
| collection | DOAJ |
| description | Abstract To evaluate the accuracy and acceptability of self-sampling samples for HPV testing for cervical cancer screening in rural Yunnan of China. In 2022, 3000 women aged 17–69 were recruited and provided self-sampling vaginal samples alongside provider-sampling samples for HPV DNA and E6/E7 mRNA testing, as well as artificial intelligence (AI)-assisted cytology. Women who tested positive for any high-risk HPV DNA or mRNA, or who displayed cytology abnormalities, were recalled for a colposcopy examination and biopsied when necessary. The accuracy of these tests in detecting cervical intraepithelial neoplasia grade 2 or worse (CIN2 +) and CIN3 + was evaluated. The sensitivity of the HPV DNA test for self-sampling was 96.2%, compared to 92.3% for provider-sampling, 73.1% for the mRNA test, and 53.9% for AI-assisted cytology in detecting CIN2 + . The specificity was 83.9% for self-sampling HPV DNA test, 86.4% for provider-sampling HPV DNA test, 91.4% for the mRNA test, and 94.2% for AI-assisted cytology. The area under the curve (AUC) values for these screening methods were 0.90, 0.90, 0.82, and 0.74, respectively. Only 37% of participants preferred the self-sampling model, with lower preference observed among older women, those from rare minority groups, those with lower education levels, and who displayed limited knowledge, attitudes, and practices regarding cervical cancer screening. Self-sampling HPV DNA testing demonstrated comparable clinical accuracy to provider-sampling test and were more accurate than that of mRNA test and AI-assisted cytology. Self-sampling HPV testing offers a promising screening strategy in rural Yunnan China. Applicable health education is urgently needed to improve the acceptability of ‘self-sampling’ model among the target population. |
| format | Article |
| id | doaj-art-adbc76f0df5e49fcb241b0e1439b20d0 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-adbc76f0df5e49fcb241b0e1439b20d02025-08-20T03:46:07ZengNature PortfolioScientific Reports2045-23222025-07-011511910.1038/s41598-025-09292-5Accuracy and acceptability of self-sampling HPV testing in cervical cancer screening: a population-based study in rural Yunnan, ChinaRufei Duan0Hongying Yang1Xiuwei Zhai2Jing Yu3Chen Lu4Wenmin Yang5Tingfeng Long6Yan Dao7Gaofeng Li8Yongchun Zhou9Hongyi Zheng10Fanghui Zhao11Yong-Tang Zheng12Yunchao Huang13Department of Cancer Prevention and Control, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital YunnanDepartment of Gynecology, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital YunnanDepartment of Medical Laboratory, The Second Affiliated Hospital of Kunming Medical UniversityDepartment of Gynecology, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital YunnanDepartment of Gynecology, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital YunnanDepartment of Gynecology, Shuangjiang Maternal and Child Health HospitalDepartment of Teaching Management, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital YunnanDepartment of Gynecology, Shuangjiang Maternal and Child Health HospitalDepartment of Cancer Prevention and Control, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital YunnanDepartment of Cancer Prevention and Control, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital YunnanKey Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of SciencesDepartment of Cancer Epidemiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeKey Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of SciencesDepartment of Cancer Prevention and Control, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital YunnanAbstract To evaluate the accuracy and acceptability of self-sampling samples for HPV testing for cervical cancer screening in rural Yunnan of China. In 2022, 3000 women aged 17–69 were recruited and provided self-sampling vaginal samples alongside provider-sampling samples for HPV DNA and E6/E7 mRNA testing, as well as artificial intelligence (AI)-assisted cytology. Women who tested positive for any high-risk HPV DNA or mRNA, or who displayed cytology abnormalities, were recalled for a colposcopy examination and biopsied when necessary. The accuracy of these tests in detecting cervical intraepithelial neoplasia grade 2 or worse (CIN2 +) and CIN3 + was evaluated. The sensitivity of the HPV DNA test for self-sampling was 96.2%, compared to 92.3% for provider-sampling, 73.1% for the mRNA test, and 53.9% for AI-assisted cytology in detecting CIN2 + . The specificity was 83.9% for self-sampling HPV DNA test, 86.4% for provider-sampling HPV DNA test, 91.4% for the mRNA test, and 94.2% for AI-assisted cytology. The area under the curve (AUC) values for these screening methods were 0.90, 0.90, 0.82, and 0.74, respectively. Only 37% of participants preferred the self-sampling model, with lower preference observed among older women, those from rare minority groups, those with lower education levels, and who displayed limited knowledge, attitudes, and practices regarding cervical cancer screening. Self-sampling HPV DNA testing demonstrated comparable clinical accuracy to provider-sampling test and were more accurate than that of mRNA test and AI-assisted cytology. Self-sampling HPV testing offers a promising screening strategy in rural Yunnan China. Applicable health education is urgently needed to improve the acceptability of ‘self-sampling’ model among the target population.https://doi.org/10.1038/s41598-025-09292-5Self-samplingHPVAccuracyAcceptabilityCervical cancer screening |
| spellingShingle | Rufei Duan Hongying Yang Xiuwei Zhai Jing Yu Chen Lu Wenmin Yang Tingfeng Long Yan Dao Gaofeng Li Yongchun Zhou Hongyi Zheng Fanghui Zhao Yong-Tang Zheng Yunchao Huang Accuracy and acceptability of self-sampling HPV testing in cervical cancer screening: a population-based study in rural Yunnan, China Scientific Reports Self-sampling HPV Accuracy Acceptability Cervical cancer screening |
| title | Accuracy and acceptability of self-sampling HPV testing in cervical cancer screening: a population-based study in rural Yunnan, China |
| title_full | Accuracy and acceptability of self-sampling HPV testing in cervical cancer screening: a population-based study in rural Yunnan, China |
| title_fullStr | Accuracy and acceptability of self-sampling HPV testing in cervical cancer screening: a population-based study in rural Yunnan, China |
| title_full_unstemmed | Accuracy and acceptability of self-sampling HPV testing in cervical cancer screening: a population-based study in rural Yunnan, China |
| title_short | Accuracy and acceptability of self-sampling HPV testing in cervical cancer screening: a population-based study in rural Yunnan, China |
| title_sort | accuracy and acceptability of self sampling hpv testing in cervical cancer screening a population based study in rural yunnan china |
| topic | Self-sampling HPV Accuracy Acceptability Cervical cancer screening |
| url | https://doi.org/10.1038/s41598-025-09292-5 |
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