Impact of <i>Kickxia elatine</i> In Vitro-Derived Stem Cells on the Biophysical Properties of Facial Skin: A Placebo-Controlled Trial

The growing demand for natural and sustainable skincare products has driven interest in plant-based active ingredients, especially from in vitro cultures. This placebo-controlled study investigated the impact of a facial cream containing 2% <i>Kickxia elatine</i> (L.) Dumort cell suspens...

Full description

Saved in:
Bibliographic Details
Main Authors: Anastasia Aliesa Hermosaningtyas, Anna Kroma-Szal, Justyna Gornowicz-Porowska, Maria Urbanska, Anna Budzianowska, Małgorzata Kikowska
Format: Article
Language:English
Published: MDPI AG 2025-08-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/15/15/8625
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849770910769217536
author Anastasia Aliesa Hermosaningtyas
Anna Kroma-Szal
Justyna Gornowicz-Porowska
Maria Urbanska
Anna Budzianowska
Małgorzata Kikowska
author_facet Anastasia Aliesa Hermosaningtyas
Anna Kroma-Szal
Justyna Gornowicz-Porowska
Maria Urbanska
Anna Budzianowska
Małgorzata Kikowska
author_sort Anastasia Aliesa Hermosaningtyas
collection DOAJ
description The growing demand for natural and sustainable skincare products has driven interest in plant-based active ingredients, especially from in vitro cultures. This placebo-controlled study investigated the impact of a facial cream containing 2% <i>Kickxia elatine</i> (L.) Dumort cell suspension culture extract on various skin biophysical parameters. The cream was applied to the cheek once daily for six weeks on 40 healthy female volunteers between the ages of 40 to 49. The evaluated skin parameters including skin hydration, transepidermal water loss (TEWL), erythema intensity (EI), melanin intensity (MI), skin surface pH, and skin structure, wrinkle depth, vascular lesions, and vascular discolouration. The results indicated that significant improvements were observed in skin hydration (from 40.36 to 63.00 AU, <i>p</i> < 0.001) and there was a decrease in TEWL score (14.82 to 11.76 g/h/m<sup>2</sup>, <i>p</i> < 0.001), while the skin surface pH was maintained (14.82 to 11.76 g/h/m<sup>2</sup>, <i>p</i> < 0.001). Moreover, the <i>K. elatine</i> cell extract significantly improved skin structure values (9.23 to 8.50, <i>p</i> = 0.028), reduced vascular lesions (2.72 to 1.54 mm<sup>2</sup>, <i>p</i> = 0.011), and lowered skin discolouration (20.98% to 14.84%, <i>p</i> < 0.001), indicating its moisturising, protective, brightening, and soothing properties. These findings support the potential use of <i>K. elatine</i> cell extract in dermocosmetic formulations targeting dry, sensitive, or ageing skin.
format Article
id doaj-art-adb8fa7a8a424e95945dab7dc530de3c
institution DOAJ
issn 2076-3417
language English
publishDate 2025-08-01
publisher MDPI AG
record_format Article
series Applied Sciences
spelling doaj-art-adb8fa7a8a424e95945dab7dc530de3c2025-08-20T03:02:48ZengMDPI AGApplied Sciences2076-34172025-08-011515862510.3390/app15158625Impact of <i>Kickxia elatine</i> In Vitro-Derived Stem Cells on the Biophysical Properties of Facial Skin: A Placebo-Controlled TrialAnastasia Aliesa Hermosaningtyas0Anna Kroma-Szal1Justyna Gornowicz-Porowska2Maria Urbanska3Anna Budzianowska4Małgorzata Kikowska5Doctoral School, Poznan University of Medical Sciences, 70 Bukowska St., 60-812 Poznan, PolandDepartment and Division of Practical Cosmetology and Skin Disease Prophylaxis, Poznan University of Medical Sciences, Collegium Pharmaceuticum, 3 Rokietnicka St., 60-806 Poznan, PolandDepartment and Division of Practical Cosmetology and Skin Disease Prophylaxis, Poznan University of Medical Sciences, Collegium Pharmaceuticum, 3 Rokietnicka St., 60-806 Poznan, PolandDepartment and Division of Practical Cosmetology and Skin Disease Prophylaxis, Poznan University of Medical Sciences, Collegium Pharmaceuticum, 3 Rokietnicka St., 60-806 Poznan, PolandLaboratory of Pharmaceutical Biology and Biotechnology, Department and Division of Practical Cosmetology and Skin Disease Prophylaxis, Poznan University of Medical Sciences, Collegium Pharmaceuticum, 3 Rokietnicka St., 60-806 Poznan, PolandLaboratory of Pharmaceutical Biology and Biotechnology, Department and Division of Practical Cosmetology and Skin Disease Prophylaxis, Poznan University of Medical Sciences, Collegium Pharmaceuticum, 3 Rokietnicka St., 60-806 Poznan, PolandThe growing demand for natural and sustainable skincare products has driven interest in plant-based active ingredients, especially from in vitro cultures. This placebo-controlled study investigated the impact of a facial cream containing 2% <i>Kickxia elatine</i> (L.) Dumort cell suspension culture extract on various skin biophysical parameters. The cream was applied to the cheek once daily for six weeks on 40 healthy female volunteers between the ages of 40 to 49. The evaluated skin parameters including skin hydration, transepidermal water loss (TEWL), erythema intensity (EI), melanin intensity (MI), skin surface pH, and skin structure, wrinkle depth, vascular lesions, and vascular discolouration. The results indicated that significant improvements were observed in skin hydration (from 40.36 to 63.00 AU, <i>p</i> < 0.001) and there was a decrease in TEWL score (14.82 to 11.76 g/h/m<sup>2</sup>, <i>p</i> < 0.001), while the skin surface pH was maintained (14.82 to 11.76 g/h/m<sup>2</sup>, <i>p</i> < 0.001). Moreover, the <i>K. elatine</i> cell extract significantly improved skin structure values (9.23 to 8.50, <i>p</i> = 0.028), reduced vascular lesions (2.72 to 1.54 mm<sup>2</sup>, <i>p</i> = 0.011), and lowered skin discolouration (20.98% to 14.84%, <i>p</i> < 0.001), indicating its moisturising, protective, brightening, and soothing properties. These findings support the potential use of <i>K. elatine</i> cell extract in dermocosmetic formulations targeting dry, sensitive, or ageing skin.https://www.mdpi.com/2076-3417/15/15/8625<i>Linaria elatine</i>cell suspension cultureplant stem cellsphytocompoundsphytocosmetic
spellingShingle Anastasia Aliesa Hermosaningtyas
Anna Kroma-Szal
Justyna Gornowicz-Porowska
Maria Urbanska
Anna Budzianowska
Małgorzata Kikowska
Impact of <i>Kickxia elatine</i> In Vitro-Derived Stem Cells on the Biophysical Properties of Facial Skin: A Placebo-Controlled Trial
Applied Sciences
<i>Linaria elatine</i>
cell suspension culture
plant stem cells
phytocompounds
phytocosmetic
title Impact of <i>Kickxia elatine</i> In Vitro-Derived Stem Cells on the Biophysical Properties of Facial Skin: A Placebo-Controlled Trial
title_full Impact of <i>Kickxia elatine</i> In Vitro-Derived Stem Cells on the Biophysical Properties of Facial Skin: A Placebo-Controlled Trial
title_fullStr Impact of <i>Kickxia elatine</i> In Vitro-Derived Stem Cells on the Biophysical Properties of Facial Skin: A Placebo-Controlled Trial
title_full_unstemmed Impact of <i>Kickxia elatine</i> In Vitro-Derived Stem Cells on the Biophysical Properties of Facial Skin: A Placebo-Controlled Trial
title_short Impact of <i>Kickxia elatine</i> In Vitro-Derived Stem Cells on the Biophysical Properties of Facial Skin: A Placebo-Controlled Trial
title_sort impact of i kickxia elatine i in vitro derived stem cells on the biophysical properties of facial skin a placebo controlled trial
topic <i>Linaria elatine</i>
cell suspension culture
plant stem cells
phytocompounds
phytocosmetic
url https://www.mdpi.com/2076-3417/15/15/8625
work_keys_str_mv AT anastasiaaliesahermosaningtyas impactofikickxiaelatineiinvitroderivedstemcellsonthebiophysicalpropertiesoffacialskinaplacebocontrolledtrial
AT annakromaszal impactofikickxiaelatineiinvitroderivedstemcellsonthebiophysicalpropertiesoffacialskinaplacebocontrolledtrial
AT justynagornowiczporowska impactofikickxiaelatineiinvitroderivedstemcellsonthebiophysicalpropertiesoffacialskinaplacebocontrolledtrial
AT mariaurbanska impactofikickxiaelatineiinvitroderivedstemcellsonthebiophysicalpropertiesoffacialskinaplacebocontrolledtrial
AT annabudzianowska impactofikickxiaelatineiinvitroderivedstemcellsonthebiophysicalpropertiesoffacialskinaplacebocontrolledtrial
AT małgorzatakikowska impactofikickxiaelatineiinvitroderivedstemcellsonthebiophysicalpropertiesoffacialskinaplacebocontrolledtrial