New Sample Preparation Method for Quantification of Phenolic Compounds of Tea (Camellia sinensis L. Kuntze): A Polyphenol Rich Plant
Chemical analysis of the Sri Lankan tea (Camellia sinensis, L.) germplasm would immensely contribute to the success of the tea breeding programme. However, the polyphenols, particularly catechins (flavan-3-ols), are readily prone to oxidation in the conventional method of sample preparation. Therefo...
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| Format: | Article |
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Wiley
2015-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/964341 |
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| author | P. A. Nimal Punyasiri Brasathe Jeganathan Jeevan Dananjaya Kottawa-Arachchi Mahasen A. B. Ranatunga I. Sarath B. Abeysinghe M. T. Kumudini Gunasekare B. M. Rathnayake Bandara |
| author_facet | P. A. Nimal Punyasiri Brasathe Jeganathan Jeevan Dananjaya Kottawa-Arachchi Mahasen A. B. Ranatunga I. Sarath B. Abeysinghe M. T. Kumudini Gunasekare B. M. Rathnayake Bandara |
| author_sort | P. A. Nimal Punyasiri |
| collection | DOAJ |
| description | Chemical analysis of the Sri Lankan tea (Camellia sinensis, L.) germplasm would immensely contribute to the success of the tea breeding programme. However, the polyphenols, particularly catechins (flavan-3-ols), are readily prone to oxidation in the conventional method of sample preparation. Therefore, optimization of the present sample preparation methodology for the profiling of metabolites is much important. Two sample preparation methodologies were compared, fresh leaves (as in the conventional procedures) and freeze-dried leaves (a new procedure), for quantification of major metabolites by employing two cultivars, one is known to be high quality black tea and the other low quality black tea. The amounts of major metabolites such as catechins, caffeine, gallic acid, and theobromine, recorded in the new sampling procedure via freeze-dried leaves, were significantly higher than those recorded in the conventional sample preparation procedure. Additionally new method required less amount of leaf sample for analysis of major metabolites and facilitates storage of samples until analysis. The freeze-dried method would be useful for high throughput analysis of large number of samples in shorter period without chemical deterioration starting from the point of harvest until usage. Hence, this method is more suitable for metabolite profiling of tea as well as other phenol rich plants. |
| format | Article |
| id | doaj-art-adb2b17b074344a981e6b79bc68ef708 |
| institution | OA Journals |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-adb2b17b074344a981e6b79bc68ef7082025-08-20T02:20:49ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732015-01-01201510.1155/2015/964341964341New Sample Preparation Method for Quantification of Phenolic Compounds of Tea (Camellia sinensis L. Kuntze): A Polyphenol Rich PlantP. A. Nimal Punyasiri0Brasathe Jeganathan1Jeevan Dananjaya Kottawa-Arachchi2Mahasen A. B. Ranatunga3I. Sarath B. Abeysinghe4M. T. Kumudini Gunasekare5B. M. Rathnayake Bandara6Institute of Biochemistry Molecular Biology and Biotechnology, University of Colombo, 00300 Colombo, Sri LankaDepartment of Food Science and Technology, Faculty of Agriculture, University of Peradeniya, 20400 Peradeniya, Sri LankaTea Research Institute of Sri Lanka, 22100 Talawakelle, Sri LankaTea Research Institute of Sri Lanka, 22100 Talawakelle, Sri LankaTea Research Institute of Sri Lanka, 22100 Talawakelle, Sri LankaCoordinating Secretariat for Science, Technology & Innovation, 3rd Floor, Standard Charted Building, Janadhipathi Mawatha, 00100 Colombo, Sri LankaDepartment of Chemistry, Faculty of Science, University of Peradeniya, 20400 Peradeniya, Sri LankaChemical analysis of the Sri Lankan tea (Camellia sinensis, L.) germplasm would immensely contribute to the success of the tea breeding programme. However, the polyphenols, particularly catechins (flavan-3-ols), are readily prone to oxidation in the conventional method of sample preparation. Therefore, optimization of the present sample preparation methodology for the profiling of metabolites is much important. Two sample preparation methodologies were compared, fresh leaves (as in the conventional procedures) and freeze-dried leaves (a new procedure), for quantification of major metabolites by employing two cultivars, one is known to be high quality black tea and the other low quality black tea. The amounts of major metabolites such as catechins, caffeine, gallic acid, and theobromine, recorded in the new sampling procedure via freeze-dried leaves, were significantly higher than those recorded in the conventional sample preparation procedure. Additionally new method required less amount of leaf sample for analysis of major metabolites and facilitates storage of samples until analysis. The freeze-dried method would be useful for high throughput analysis of large number of samples in shorter period without chemical deterioration starting from the point of harvest until usage. Hence, this method is more suitable for metabolite profiling of tea as well as other phenol rich plants.http://dx.doi.org/10.1155/2015/964341 |
| spellingShingle | P. A. Nimal Punyasiri Brasathe Jeganathan Jeevan Dananjaya Kottawa-Arachchi Mahasen A. B. Ranatunga I. Sarath B. Abeysinghe M. T. Kumudini Gunasekare B. M. Rathnayake Bandara New Sample Preparation Method for Quantification of Phenolic Compounds of Tea (Camellia sinensis L. Kuntze): A Polyphenol Rich Plant Journal of Analytical Methods in Chemistry |
| title | New Sample Preparation Method for Quantification of Phenolic Compounds of Tea (Camellia sinensis L. Kuntze): A Polyphenol Rich Plant |
| title_full | New Sample Preparation Method for Quantification of Phenolic Compounds of Tea (Camellia sinensis L. Kuntze): A Polyphenol Rich Plant |
| title_fullStr | New Sample Preparation Method for Quantification of Phenolic Compounds of Tea (Camellia sinensis L. Kuntze): A Polyphenol Rich Plant |
| title_full_unstemmed | New Sample Preparation Method for Quantification of Phenolic Compounds of Tea (Camellia sinensis L. Kuntze): A Polyphenol Rich Plant |
| title_short | New Sample Preparation Method for Quantification of Phenolic Compounds of Tea (Camellia sinensis L. Kuntze): A Polyphenol Rich Plant |
| title_sort | new sample preparation method for quantification of phenolic compounds of tea camellia sinensis l kuntze a polyphenol rich plant |
| url | http://dx.doi.org/10.1155/2015/964341 |
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