Cryptic variation between species and the basis of hybrid performance.

Crosses between closely related species give two contrasting results. One result is that species hybrids may be inferior to their parents, for example, being less fertile [1]. The other is that F1 hybrids may display superior performance (heterosis), for example with increased vigour [2]. Although v...

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Main Authors: Ulises Rosas, Nick H Barton, Lucy Copsey, Pierre Barbier de Reuille, Enrico Coen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-07-01
Series:PLoS Biology
Online Access:https://doi.org/10.1371/journal.pbio.1000429
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author Ulises Rosas
Nick H Barton
Lucy Copsey
Pierre Barbier de Reuille
Enrico Coen
author_facet Ulises Rosas
Nick H Barton
Lucy Copsey
Pierre Barbier de Reuille
Enrico Coen
author_sort Ulises Rosas
collection DOAJ
description Crosses between closely related species give two contrasting results. One result is that species hybrids may be inferior to their parents, for example, being less fertile [1]. The other is that F1 hybrids may display superior performance (heterosis), for example with increased vigour [2]. Although various hypotheses have been proposed to account for these two aspects of hybridisation, their biological basis is still poorly understood [3]. To gain further insights into this issue, we analysed the role that variation in gene expression may play. We took a conserved trait, flower asymmetry in Antirrhinum, and determined the extent to which the underlying regulatory genes varied in expression among closely related species. We show that expression of both genes analysed, CYC and RAD, varies significantly between species because of cis-acting differences. By making a quantitative genotype-phenotype map, using a range of mutant alleles, we demonstrate that the species lie on a plateau in gene expression-morphology space, so that the variation has no detectable phenotypic effect. However, phenotypic differences can be revealed by shifting genotypes off the plateau through genetic crosses. Our results can be readily explained if genomes are free to evolve within an effectively neutral zone in gene expression space. The consequences of this drift will be negligible for individual loci, but when multiple loci across the genome are considered, we show that the variation may have significant effects on phenotype and fitness, causing a significant drift load. By considering these consequences for various gene-expression-fitness landscapes, we conclude that F1 hybrids might be expected to show increased performance with regard to conserved traits, such as basic physiology, but reduced performance with regard to others. Thus, our study provides a new way of explaining how various aspects of hybrid performance may arise through natural variation in gene activity.
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spelling doaj-art-adb29b33234241b8a7a43e083d3ab3dd2025-08-20T03:19:52ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852010-07-0187e100042910.1371/journal.pbio.1000429Cryptic variation between species and the basis of hybrid performance.Ulises RosasNick H BartonLucy CopseyPierre Barbier de ReuilleEnrico CoenCrosses between closely related species give two contrasting results. One result is that species hybrids may be inferior to their parents, for example, being less fertile [1]. The other is that F1 hybrids may display superior performance (heterosis), for example with increased vigour [2]. Although various hypotheses have been proposed to account for these two aspects of hybridisation, their biological basis is still poorly understood [3]. To gain further insights into this issue, we analysed the role that variation in gene expression may play. We took a conserved trait, flower asymmetry in Antirrhinum, and determined the extent to which the underlying regulatory genes varied in expression among closely related species. We show that expression of both genes analysed, CYC and RAD, varies significantly between species because of cis-acting differences. By making a quantitative genotype-phenotype map, using a range of mutant alleles, we demonstrate that the species lie on a plateau in gene expression-morphology space, so that the variation has no detectable phenotypic effect. However, phenotypic differences can be revealed by shifting genotypes off the plateau through genetic crosses. Our results can be readily explained if genomes are free to evolve within an effectively neutral zone in gene expression space. The consequences of this drift will be negligible for individual loci, but when multiple loci across the genome are considered, we show that the variation may have significant effects on phenotype and fitness, causing a significant drift load. By considering these consequences for various gene-expression-fitness landscapes, we conclude that F1 hybrids might be expected to show increased performance with regard to conserved traits, such as basic physiology, but reduced performance with regard to others. Thus, our study provides a new way of explaining how various aspects of hybrid performance may arise through natural variation in gene activity.https://doi.org/10.1371/journal.pbio.1000429
spellingShingle Ulises Rosas
Nick H Barton
Lucy Copsey
Pierre Barbier de Reuille
Enrico Coen
Cryptic variation between species and the basis of hybrid performance.
PLoS Biology
title Cryptic variation between species and the basis of hybrid performance.
title_full Cryptic variation between species and the basis of hybrid performance.
title_fullStr Cryptic variation between species and the basis of hybrid performance.
title_full_unstemmed Cryptic variation between species and the basis of hybrid performance.
title_short Cryptic variation between species and the basis of hybrid performance.
title_sort cryptic variation between species and the basis of hybrid performance
url https://doi.org/10.1371/journal.pbio.1000429
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