Heat stress-induced CYP1A2 upregulation in sow ovarian granulosa cells
Background: Heat stress during summer impairs reproductive performance in sows, causing summer infertility. Cytochrome P450 1A2 (CYP1A2) plays a crucial role in steroid hormone metabolism in the liver and has been shown to be upregulated under stress conditions. However, the effect of seasonal heat...
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| Format: | Article |
| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2025-06-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | https://www.e-jarb.org/journal/view.html?uid=2729&vmd=Full |
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| author | Tae-Gyun Kim Hyun-Seung Lee Sung-Ho Kim Young-Bum Son Seong-Eun Heo Do-Yoon Kim Seung-Joon Kim Won-Jae Lee |
| author_facet | Tae-Gyun Kim Hyun-Seung Lee Sung-Ho Kim Young-Bum Son Seong-Eun Heo Do-Yoon Kim Seung-Joon Kim Won-Jae Lee |
| author_sort | Tae-Gyun Kim |
| collection | DOAJ |
| description | Background: Heat stress during summer impairs reproductive performance in sows, causing summer infertility. Cytochrome P450 1A2 (CYP1A2) plays a crucial role in steroid hormone metabolism in the liver and has been shown to be upregulated under stress conditions. However, the effect of seasonal heat stress on CYP1A2 expression in sow ovaries remains poorly understood.
Methods: Ovaries were collected from crossbred sows during different seasons in Korea. The control group (CON) was exposed to optimal temperatures for swine rearing (22.0-24.5℃) during spring, while the heat-stressed group (HS) experienced high temperatures (30.5-37.0℃) during summer. Temperature-humidity index (THI) was calculated to assess thermal stress severity. CYP1A2 expression was analyzed using Western blotting and immunohistochemistry (IHC), and these values were statistically compared with THI by Pearson’s correlation analysis.
Results: The HS exhibited significantly higher THI values compared to the CON (88.11 vs. 66.67). Western blot analysis revealed significant upregulation of CYP1A2 expression in the HS compared to CON (1.89 vs. 1.00). IHC demonstrated that CYP1A2 was specifically localized in granulosa cells of mature follicles, with a significantly higher proportion of CYP1A2-positive follicles in the HS (15.92%) compared to CON (9.08%). When the obtained values were compared with THI values, CYP1A2 expression showed a strong positive correlation with THI (r = 0.41 and 0.74).
Conclusions: This study revealed that heat stress can alter the ovarian microenvironment, including the expression of CYP1A2. These findings emphasize the need for effective thermal management strategies to mitigate heat stress-induced reproductive dysfunction in livestock production. |
| format | Article |
| id | doaj-art-adac31b142734bdd8be491475b0f3b0a |
| institution | Kabale University |
| issn | 2671-4639 2671-4663 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj-art-adac31b142734bdd8be491475b0f3b0a2025-08-20T03:28:37ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632025-06-01402909710.12750/JARB.40.2.90Heat stress-induced CYP1A2 upregulation in sow ovarian granulosa cellsTae-Gyun Kim0https://orcid.org/0009-0005-8222-2516Hyun-Seung Lee1https://orcid.org/0009-0002-2338-1945Sung-Ho Kim2https://orcid.org/0000-0001-9088-9840Young-Bum Son3https://orcid.org/0000-0002-9581-9339Seong-Eun Heo4https://orcid.org/0009-0007-3526-2536Do-Yoon Kim5https://orcid.org/0000-0002-3028-7706Seung-Joon Kim6https://orcid.org/0000-0002-8521-8898Won-Jae Lee7https://orcid.org/0000-0003-1462-7798College of Veterinary Medicine, Kyungpook National University, Daegu 41566, KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, KoreaCollege of Veterinary Medicine, Chonnam National University, Gwangju 61186, KoreaGyeongsangbuk-Do Livestock Research Institute, Yeongju 36052, KoreaGyeongsangbuk-Do Livestock Research Institute, Yeongju 36052, KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, KoreaBackground: Heat stress during summer impairs reproductive performance in sows, causing summer infertility. Cytochrome P450 1A2 (CYP1A2) plays a crucial role in steroid hormone metabolism in the liver and has been shown to be upregulated under stress conditions. However, the effect of seasonal heat stress on CYP1A2 expression in sow ovaries remains poorly understood. Methods: Ovaries were collected from crossbred sows during different seasons in Korea. The control group (CON) was exposed to optimal temperatures for swine rearing (22.0-24.5℃) during spring, while the heat-stressed group (HS) experienced high temperatures (30.5-37.0℃) during summer. Temperature-humidity index (THI) was calculated to assess thermal stress severity. CYP1A2 expression was analyzed using Western blotting and immunohistochemistry (IHC), and these values were statistically compared with THI by Pearson’s correlation analysis. Results: The HS exhibited significantly higher THI values compared to the CON (88.11 vs. 66.67). Western blot analysis revealed significant upregulation of CYP1A2 expression in the HS compared to CON (1.89 vs. 1.00). IHC demonstrated that CYP1A2 was specifically localized in granulosa cells of mature follicles, with a significantly higher proportion of CYP1A2-positive follicles in the HS (15.92%) compared to CON (9.08%). When the obtained values were compared with THI values, CYP1A2 expression showed a strong positive correlation with THI (r = 0.41 and 0.74). Conclusions: This study revealed that heat stress can alter the ovarian microenvironment, including the expression of CYP1A2. These findings emphasize the need for effective thermal management strategies to mitigate heat stress-induced reproductive dysfunction in livestock production.https://www.e-jarb.org/journal/view.html?uid=2729&vmd=Fullcyp1a2heat stressovarysowtemperature-humidity index |
| spellingShingle | Tae-Gyun Kim Hyun-Seung Lee Sung-Ho Kim Young-Bum Son Seong-Eun Heo Do-Yoon Kim Seung-Joon Kim Won-Jae Lee Heat stress-induced CYP1A2 upregulation in sow ovarian granulosa cells Journal of Animal Reproduction and Biotechnology cyp1a2 heat stress ovary sow temperature-humidity index |
| title | Heat stress-induced CYP1A2 upregulation in sow ovarian granulosa cells |
| title_full | Heat stress-induced CYP1A2 upregulation in sow ovarian granulosa cells |
| title_fullStr | Heat stress-induced CYP1A2 upregulation in sow ovarian granulosa cells |
| title_full_unstemmed | Heat stress-induced CYP1A2 upregulation in sow ovarian granulosa cells |
| title_short | Heat stress-induced CYP1A2 upregulation in sow ovarian granulosa cells |
| title_sort | heat stress induced cyp1a2 upregulation in sow ovarian granulosa cells |
| topic | cyp1a2 heat stress ovary sow temperature-humidity index |
| url | https://www.e-jarb.org/journal/view.html?uid=2729&vmd=Full |
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