Fusobacterium nucleatum as an oral bacteria promotes the carcinogenesis of laryngeal cancer via upregulating YWHAZ
Background and purpose F. nucleatum, a gram-negative oral bacteria, is abundant in laryngeal cancer (LC). While specific 14-3-3 proteins act as LC oncogenes, the link between F. nucleatum and 14-3-3 proteins dysregulation remains unknown.Materials and methods Transcriptome data from 520 HNSC patient...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2024.2442245 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850119588935630848 |
|---|---|
| author | Yiyue Ren Jing Ye Liuhong Shi Kehao Le Yifan Wang |
| author_facet | Yiyue Ren Jing Ye Liuhong Shi Kehao Le Yifan Wang |
| author_sort | Yiyue Ren |
| collection | DOAJ |
| description | Background and purpose F. nucleatum, a gram-negative oral bacteria, is abundant in laryngeal cancer (LC). While specific 14-3-3 proteins act as LC oncogenes, the link between F. nucleatum and 14-3-3 proteins dysregulation remains unknown.Materials and methods Transcriptome data from 520 HNSC patients were obtained from TCGA. DEGs were evaluated by Wilcoxon test, and survival analysis done by KM plotter. In 41 untreated LC patients, F. nucleatum was detected by PCR. GEO data assessed 14-3-3 protein changes post F. nucleatum infection, verified in HuLa-PC and LC cells (Tu686, LCC). YWHAZ oncogenic role was tested in vitro and in xenografts. YWHAZ knockdown confirmed F. nucleatum’s oncogenic effects are YWHAZ-dependent.Results F. nucleatum positive tumors have increased YWAHZ. F. nucleatum infection upregulates the expression of YWHAZ in HuLa-PC, Tu686 and Hep-2 cells. YWAHZ overexpression promotes proliferation, migration and colony formation but inhibits apoptosis of HuLa-PC and LCa cells via activating Akt signaling. F. nucleatum infection promotes the tumor growth. Knockdown YWAHZ in LCa cells totally rescued the phenotypes of F. nucleatum infection.Conclusions We identified for the first time that F. nucleatum infection promotes the development of LCa via upregulating YWHAZ, which provides a new clue for LCa treatment. |
| format | Article |
| id | doaj-art-adabe16d623a42adaae40a5f767c0516 |
| institution | OA Journals |
| issn | 2000-2297 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-adabe16d623a42adaae40a5f767c05162025-08-20T02:35:36ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972025-12-0117110.1080/20002297.2024.2442245Fusobacterium nucleatum as an oral bacteria promotes the carcinogenesis of laryngeal cancer via upregulating YWHAZYiyue Ren0Jing Ye1Liuhong Shi2Kehao Le3Yifan Wang4Department of Head and Neck Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, ChinaDepartment of Otolaryngology Head and Neck Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, ChinaDepartment of Head and Neck Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, ChinaDepartment of Head and Neck Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, ChinaSchool of Medicine, Zhejiang University, Hangzhou, Zhejiang, ChinaBackground and purpose F. nucleatum, a gram-negative oral bacteria, is abundant in laryngeal cancer (LC). While specific 14-3-3 proteins act as LC oncogenes, the link between F. nucleatum and 14-3-3 proteins dysregulation remains unknown.Materials and methods Transcriptome data from 520 HNSC patients were obtained from TCGA. DEGs were evaluated by Wilcoxon test, and survival analysis done by KM plotter. In 41 untreated LC patients, F. nucleatum was detected by PCR. GEO data assessed 14-3-3 protein changes post F. nucleatum infection, verified in HuLa-PC and LC cells (Tu686, LCC). YWHAZ oncogenic role was tested in vitro and in xenografts. YWHAZ knockdown confirmed F. nucleatum’s oncogenic effects are YWHAZ-dependent.Results F. nucleatum positive tumors have increased YWAHZ. F. nucleatum infection upregulates the expression of YWHAZ in HuLa-PC, Tu686 and Hep-2 cells. YWAHZ overexpression promotes proliferation, migration and colony formation but inhibits apoptosis of HuLa-PC and LCa cells via activating Akt signaling. F. nucleatum infection promotes the tumor growth. Knockdown YWAHZ in LCa cells totally rescued the phenotypes of F. nucleatum infection.Conclusions We identified for the first time that F. nucleatum infection promotes the development of LCa via upregulating YWHAZ, which provides a new clue for LCa treatment.https://www.tandfonline.com/doi/10.1080/20002297.2024.2442245Laryngeal cancerFusobacterium nucleatumYWAHZHNSCAkt |
| spellingShingle | Yiyue Ren Jing Ye Liuhong Shi Kehao Le Yifan Wang Fusobacterium nucleatum as an oral bacteria promotes the carcinogenesis of laryngeal cancer via upregulating YWHAZ Journal of Oral Microbiology Laryngeal cancer Fusobacterium nucleatum YWAHZ HNSC Akt |
| title | Fusobacterium nucleatum as an oral bacteria promotes the carcinogenesis of laryngeal cancer via upregulating YWHAZ |
| title_full | Fusobacterium nucleatum as an oral bacteria promotes the carcinogenesis of laryngeal cancer via upregulating YWHAZ |
| title_fullStr | Fusobacterium nucleatum as an oral bacteria promotes the carcinogenesis of laryngeal cancer via upregulating YWHAZ |
| title_full_unstemmed | Fusobacterium nucleatum as an oral bacteria promotes the carcinogenesis of laryngeal cancer via upregulating YWHAZ |
| title_short | Fusobacterium nucleatum as an oral bacteria promotes the carcinogenesis of laryngeal cancer via upregulating YWHAZ |
| title_sort | fusobacterium nucleatum as an oral bacteria promotes the carcinogenesis of laryngeal cancer via upregulating ywhaz |
| topic | Laryngeal cancer Fusobacterium nucleatum YWAHZ HNSC Akt |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2024.2442245 |
| work_keys_str_mv | AT yiyueren fusobacteriumnucleatumasanoralbacteriapromotesthecarcinogenesisoflaryngealcancerviaupregulatingywhaz AT jingye fusobacteriumnucleatumasanoralbacteriapromotesthecarcinogenesisoflaryngealcancerviaupregulatingywhaz AT liuhongshi fusobacteriumnucleatumasanoralbacteriapromotesthecarcinogenesisoflaryngealcancerviaupregulatingywhaz AT kehaole fusobacteriumnucleatumasanoralbacteriapromotesthecarcinogenesisoflaryngealcancerviaupregulatingywhaz AT yifanwang fusobacteriumnucleatumasanoralbacteriapromotesthecarcinogenesisoflaryngealcancerviaupregulatingywhaz |