Prevalence and genotype distribution of human papillomavirus in individuals referred to a laboratory in Rey City, Iran

Abstract Background and aim Human papillomavirus (HPV) is a common sexually transmitted infection linked to various cancers and most sexually active individuals are at risk of contracting it. Understanding regional genotype distribution is essential for establishing effective prevention programs. Th...

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Main Authors: Omid Salahi Ardekani, Nasim Bashiri, Arash Letafati, Saber Taheri, Fateme Sadat Hashemi Pasand, Mojgan Rahmanian, Alireza Shikki, Shima Sadeghipour Marvi, Mohammad Javad Lakzian, Sheida Sarrafzadeh, Nasim Rafinezhad, Behnam Molavi, Masoomeh Bahari, Tina Kahen
Format: Article
Language:English
Published: Springer 2025-05-01
Series:Discover Social Science and Health
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Online Access:https://doi.org/10.1007/s44155-025-00227-z
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author Omid Salahi Ardekani
Nasim Bashiri
Arash Letafati
Saber Taheri
Fateme Sadat Hashemi Pasand
Mojgan Rahmanian
Alireza Shikki
Shima Sadeghipour Marvi
Mohammad Javad Lakzian
Sheida Sarrafzadeh
Nasim Rafinezhad
Behnam Molavi
Masoomeh Bahari
Tina Kahen
author_facet Omid Salahi Ardekani
Nasim Bashiri
Arash Letafati
Saber Taheri
Fateme Sadat Hashemi Pasand
Mojgan Rahmanian
Alireza Shikki
Shima Sadeghipour Marvi
Mohammad Javad Lakzian
Sheida Sarrafzadeh
Nasim Rafinezhad
Behnam Molavi
Masoomeh Bahari
Tina Kahen
author_sort Omid Salahi Ardekani
collection DOAJ
description Abstract Background and aim Human papillomavirus (HPV) is a common sexually transmitted infection linked to various cancers and most sexually active individuals are at risk of contracting it. Understanding regional genotype distribution is essential for establishing effective prevention programs. Therefore, this research focused on analyzing the distribution of HPV genotypes among individuals referred to a laboratory in Rey City, Iran. Material and method This study, conducted from September to December 2024 in Rey City, Iran, analyzed genital specimens from female patients. The Favorgen Biotech Corp kit (FAVORGEN, Taiwan) was used for DNA extraction, and the CLART® HPV4 kit (GENOMICA, Spain) was used for PCR and genotyping. Data were analyzed at individual and genotype levels using frequency analysis and statistical analysis was performed with IBM SPSS-27. Results Out of the 722 females tested, 195 (27%) were found to be HPV-positive. Among those infected, 29.2% carried only high-risk (HR) genotypes, while over 36.9% had only low-risk (LR) genotypes. Additionally, 23.6% of individuals tested positive for both HR and LR genotypes. The prevalence of HR genotypes was highest among individuals aged 25 to 34 (54%). The genotypes that appeared most frequently included HPV-6 (13.8%), HPV-16 (7.4%), HPV-39 (6.6%), and HPV-54 (6.3%), while HPV-26 and HPV-43 were each detected in only one case. Conclusion This study found a significant HPV prevalence in females in Rey City, especially those under 25 and aged 25–34, highlighting the need for age-targeted prevention. Since current vaccines do not cover HPV-39 and HPV-54, the findings provide valuable data for future vaccination policies in Rey City, Iran.
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spelling doaj-art-adab482f1ecb45548892fca75394eb592025-08-20T01:53:15ZengSpringerDiscover Social Science and Health2731-04692025-05-015111210.1007/s44155-025-00227-zPrevalence and genotype distribution of human papillomavirus in individuals referred to a laboratory in Rey City, IranOmid Salahi Ardekani0Nasim Bashiri1Arash Letafati2Saber Taheri3Fateme Sadat Hashemi Pasand4Mojgan Rahmanian5Alireza Shikki6Shima Sadeghipour Marvi7Mohammad Javad Lakzian8Sheida Sarrafzadeh9Nasim Rafinezhad10Behnam Molavi11Masoomeh Bahari12Tina Kahen13Research Center for Clinical Virology, Tehran University of Medical SciencesStudent Scientific Research Center (SSRC), Tehran University of Medical SciencesStudent Scientific Research Center (SSRC), Tehran University of Medical SciencesStudent Scientific Research Center (SSRC), Tehran University of Medical SciencesResearch Center for Clinical Virology, Tehran University of Medical SciencesAbnormal Uterine Bleeding Research Center, Semnan University of Medical SciencesDepartment of Microbiology, Shahed UniversityResearch Center for Clinical Virology, Tehran University of Medical SciencesDepartment of Laboratory Science, Faculty of Paramedicine, Mashhad Medical Science, Islamic Azad UniversityResearch Center for Clinical Virology, Tehran University of Medical SciencesResearch Center for Clinical Virology, Tehran University of Medical SciencesResearch Center for Immunodeficiencies, Children’s Medical Center, Tehran University of Medical Sciences (TUMS)Research Center for Clinical Virology, Tehran University of Medical SciencesResearch Center for Clinical Virology, Tehran University of Medical SciencesAbstract Background and aim Human papillomavirus (HPV) is a common sexually transmitted infection linked to various cancers and most sexually active individuals are at risk of contracting it. Understanding regional genotype distribution is essential for establishing effective prevention programs. Therefore, this research focused on analyzing the distribution of HPV genotypes among individuals referred to a laboratory in Rey City, Iran. Material and method This study, conducted from September to December 2024 in Rey City, Iran, analyzed genital specimens from female patients. The Favorgen Biotech Corp kit (FAVORGEN, Taiwan) was used for DNA extraction, and the CLART® HPV4 kit (GENOMICA, Spain) was used for PCR and genotyping. Data were analyzed at individual and genotype levels using frequency analysis and statistical analysis was performed with IBM SPSS-27. Results Out of the 722 females tested, 195 (27%) were found to be HPV-positive. Among those infected, 29.2% carried only high-risk (HR) genotypes, while over 36.9% had only low-risk (LR) genotypes. Additionally, 23.6% of individuals tested positive for both HR and LR genotypes. The prevalence of HR genotypes was highest among individuals aged 25 to 34 (54%). The genotypes that appeared most frequently included HPV-6 (13.8%), HPV-16 (7.4%), HPV-39 (6.6%), and HPV-54 (6.3%), while HPV-26 and HPV-43 were each detected in only one case. Conclusion This study found a significant HPV prevalence in females in Rey City, especially those under 25 and aged 25–34, highlighting the need for age-targeted prevention. Since current vaccines do not cover HPV-39 and HPV-54, the findings provide valuable data for future vaccination policies in Rey City, Iran.https://doi.org/10.1007/s44155-025-00227-zHuman papillomavirus (HPV)HPV prevalenceGenotype distributionVaccinePrevention
spellingShingle Omid Salahi Ardekani
Nasim Bashiri
Arash Letafati
Saber Taheri
Fateme Sadat Hashemi Pasand
Mojgan Rahmanian
Alireza Shikki
Shima Sadeghipour Marvi
Mohammad Javad Lakzian
Sheida Sarrafzadeh
Nasim Rafinezhad
Behnam Molavi
Masoomeh Bahari
Tina Kahen
Prevalence and genotype distribution of human papillomavirus in individuals referred to a laboratory in Rey City, Iran
Discover Social Science and Health
Human papillomavirus (HPV)
HPV prevalence
Genotype distribution
Vaccine
Prevention
title Prevalence and genotype distribution of human papillomavirus in individuals referred to a laboratory in Rey City, Iran
title_full Prevalence and genotype distribution of human papillomavirus in individuals referred to a laboratory in Rey City, Iran
title_fullStr Prevalence and genotype distribution of human papillomavirus in individuals referred to a laboratory in Rey City, Iran
title_full_unstemmed Prevalence and genotype distribution of human papillomavirus in individuals referred to a laboratory in Rey City, Iran
title_short Prevalence and genotype distribution of human papillomavirus in individuals referred to a laboratory in Rey City, Iran
title_sort prevalence and genotype distribution of human papillomavirus in individuals referred to a laboratory in rey city iran
topic Human papillomavirus (HPV)
HPV prevalence
Genotype distribution
Vaccine
Prevention
url https://doi.org/10.1007/s44155-025-00227-z
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