The beneficial effects of thymoquinone on viability and differentiation of human adipose tissue-derived mesenchymal stem cells
Thymoquinone is the primary active substance extracted from Nigella sativa. It has been reported to have many beneficial and protective effects on several cell types. However, there are limited studies about its effects on mesenchymal stem cells (MSCs). The purpose of this study is to investigate th...
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| Language: | English |
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Society of Turaz Bilim
2024-04-01
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| Series: | Medicine Science |
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| Online Access: | https://www.medicinescience.org/?mno=222357 |
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| author | Ahsen Erginsoy Nur Seda Gokdemir Venhar Cinar Eda Koseoglu Zeynep Burcin Gonen Tuba Dilay Kokenek Unal |
| author_facet | Ahsen Erginsoy Nur Seda Gokdemir Venhar Cinar Eda Koseoglu Zeynep Burcin Gonen Tuba Dilay Kokenek Unal |
| author_sort | Ahsen Erginsoy |
| collection | DOAJ |
| description | Thymoquinone is the primary active substance extracted from Nigella sativa. It has been reported to have many beneficial and protective effects on several cell types. However, there are limited studies about its effects on mesenchymal stem cells (MSCs). The purpose of this study is to investigate the effect of thymoquinone on the cell viability, proliferation, and differentiation of MSCs obtained from human adipose tissue. MSCs were obtained from the biobank and treated with concentrations of 50 nM, 100 nM, 200 nM, and 300 nM thymoquinone. Cell viability, proliferation, and apoptosis analysis were performed. The effects of thymoquinone on the differentiation of human adipose tissue-derived mesenchymal stem cells (haMSCs) were investigated through adipogenic, osteogenic, and chondrogenic assays. As a result of our study, doses of 50 nM, 100 nM, 200 nM, and 300 nM thymoquinone have been demonstrated to enhance cell proliferation, prevent apoptosis, and contribute to adipogenic, chondrogenic, and osteogenic differentiation. Thymoquinone promotes the viability of haMSCs and induces differentiation into osteogenic, adipogenic, and chondrogenic lienages. Therefore, it is an auxiliary active ingredient that can be used safely in regenerative medicine. [Med-Science 2024; 13(4.000): 973-8] |
| format | Article |
| id | doaj-art-ada5e69695b24c6d840e958f4d3524e7 |
| institution | Kabale University |
| issn | 2147-0634 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Society of Turaz Bilim |
| record_format | Article |
| series | Medicine Science |
| spelling | doaj-art-ada5e69695b24c6d840e958f4d3524e72025-02-07T08:49:24ZengSociety of Turaz BilimMedicine Science2147-06342024-04-01134973810.5455/medscience.2024.09.115222357The beneficial effects of thymoquinone on viability and differentiation of human adipose tissue-derived mesenchymal stem cellsAhsen Erginsoy0Nur Seda Gokdemir1Venhar Cinar2Eda Koseoglu3Zeynep Burcin Gonen4Tuba Dilay Kokenek Unal5Department of Stem Cell, Betul Ziya Eren Genome and Stem Cell Centre, Erciyes University, Kayseri, Turkiye Department of Stem Cell, Betul Ziya Eren Genome and Stem Cell Centre, Erciyes University, Kayseri, Turkiye Department of Medical Biology, Faculty of Medicine, Erciyes University, Kayseri, Turkiye Department of Histology and Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkiye Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Erciyes University, Kayseri, Turkey Department of Pathology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, TurkiyeThymoquinone is the primary active substance extracted from Nigella sativa. It has been reported to have many beneficial and protective effects on several cell types. However, there are limited studies about its effects on mesenchymal stem cells (MSCs). The purpose of this study is to investigate the effect of thymoquinone on the cell viability, proliferation, and differentiation of MSCs obtained from human adipose tissue. MSCs were obtained from the biobank and treated with concentrations of 50 nM, 100 nM, 200 nM, and 300 nM thymoquinone. Cell viability, proliferation, and apoptosis analysis were performed. The effects of thymoquinone on the differentiation of human adipose tissue-derived mesenchymal stem cells (haMSCs) were investigated through adipogenic, osteogenic, and chondrogenic assays. As a result of our study, doses of 50 nM, 100 nM, 200 nM, and 300 nM thymoquinone have been demonstrated to enhance cell proliferation, prevent apoptosis, and contribute to adipogenic, chondrogenic, and osteogenic differentiation. Thymoquinone promotes the viability of haMSCs and induces differentiation into osteogenic, adipogenic, and chondrogenic lienages. Therefore, it is an auxiliary active ingredient that can be used safely in regenerative medicine. [Med-Science 2024; 13(4.000): 973-8]https://www.medicinescience.org/?mno=222357thymoquinonecell differentiationhuman adipose tissue-derived mesenchymal stem cell |
| spellingShingle | Ahsen Erginsoy Nur Seda Gokdemir Venhar Cinar Eda Koseoglu Zeynep Burcin Gonen Tuba Dilay Kokenek Unal The beneficial effects of thymoquinone on viability and differentiation of human adipose tissue-derived mesenchymal stem cells Medicine Science thymoquinone cell differentiation human adipose tissue-derived mesenchymal stem cell |
| title | The beneficial effects of thymoquinone on viability and differentiation of human adipose tissue-derived mesenchymal stem cells |
| title_full | The beneficial effects of thymoquinone on viability and differentiation of human adipose tissue-derived mesenchymal stem cells |
| title_fullStr | The beneficial effects of thymoquinone on viability and differentiation of human adipose tissue-derived mesenchymal stem cells |
| title_full_unstemmed | The beneficial effects of thymoquinone on viability and differentiation of human adipose tissue-derived mesenchymal stem cells |
| title_short | The beneficial effects of thymoquinone on viability and differentiation of human adipose tissue-derived mesenchymal stem cells |
| title_sort | beneficial effects of thymoquinone on viability and differentiation of human adipose tissue derived mesenchymal stem cells |
| topic | thymoquinone cell differentiation human adipose tissue-derived mesenchymal stem cell |
| url | https://www.medicinescience.org/?mno=222357 |
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