Effect of Chlorhexidine and Benincasa Hispida Pretreatment on Microshear Bond Strength of Universal Adhesive System on Dentin: A Pilot Study
Introduction: Achieving strong bond strength of adhesive systems to deep dentin is challenging. Various methods to improve bond strength to deep dentin have been investigated. Chlorhexidine (CHX) enhances the longevity of the bond between adhesives and dentin by inhibiting the collagenolytic activit...
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JCDR Research and Publications Private Limited
2024-11-01
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| author | Girija S Sajjan Manda Sri Swathi P Arun Bhupathi Madhu Varma Kanumuri Rajulapati Kalyan Satish Rama Krishna Alla Tippireddy Srija Ch Uma Shalini |
| author_facet | Girija S Sajjan Manda Sri Swathi P Arun Bhupathi Madhu Varma Kanumuri Rajulapati Kalyan Satish Rama Krishna Alla Tippireddy Srija Ch Uma Shalini |
| author_sort | Girija S Sajjan |
| collection | DOAJ |
| description | Introduction: Achieving strong bond strength of adhesive systems to deep dentin is challenging. Various methods to improve bond strength to deep dentin have been investigated. Chlorhexidine (CHX) enhances the longevity of the bond between adhesives and dentin by inhibiting the collagenolytic activity of Matrix Metalloproteinases (MMPs). Benincasa hispida is a natural plant-based functional food containing minerals and vitamins that help prevent osteoporosis and improve bone health.
Aim: To evaluate the Microshear Bond Strength (MSBS) of a 10-methacryloyloxydecyl-dihydrogen Phosphate-based (10-MDP) universal adhesive system to coronal dentin with different dentin surface pretreatments, specifically CHX and Benincasa hispida.
Materials and Methods: An in-vitro pilot study was conducted at the Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India, from May 2023 to November 2023. Dentin discs (42) were prepared from human mandibular first molars. A standard etch-and-rinse protocol was performed on the discs. The specimens were assigned to different dentin pretreatments: Group-I (no pretreatment), Group-II (CHX) and Group-III (Benincasa hispida). CHX and Benincasa hispida were applied using micro applicator tips. A dentin bonding agent containing 10-MDP (DBA) was applied, photopolymerised and a composite restoration was fabricated. The MSBS to dentin was evaluated using a universal testing machine. The failure patterns were analysed using a stereomicroscope at 10x magnification. One-way Analysis of Variance (ANOVA) and Tukey’s Honestly Significant Difference (HSD) HSD tests were used for statistical analysis, with a significance level set at α=0.05.
Results: The mean MSBS values were 24.2732±5.41329 MPa for Group-III, 14.3848±4.23492 MPa for Group-II and 6.9724±3.15837 MPa for Group-I. Pretreatment of dentin with B. hispida resulted in significantly higher MSBS values compared to the other two groups (p<0.001*).
Conclusion: Pretreatment with B. hispida significantly improved MSBS. Thus, B. hispida pretreatment may be beneficial in increasing dentin bond strength. |
| format | Article |
| id | doaj-art-ad95028bc3cf4645804c583b7fbb9a50 |
| institution | OA Journals |
| issn | 2249-782X 0973-709X |
| language | English |
| publishDate | 2024-11-01 |
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| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj-art-ad95028bc3cf4645804c583b7fbb9a502025-08-20T02:26:35ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2024-11-011811010610.7860/JCDR/2024/73389.20224Effect of Chlorhexidine and Benincasa Hispida Pretreatment on Microshear Bond Strength of Universal Adhesive System on Dentin: A Pilot StudyGirija S Sajjan0Manda Sri Swathi1P Arun Bhupathi2Madhu Varma Kanumuri3Rajulapati Kalyan Satish4Rama Krishna Alla5Tippireddy Srija6Ch Uma Shalini7Head, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Postgraduate Student, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Assistant Professor, Department of Orthodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Professor, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Professor, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Professor, Department of Dental Materials, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Postgraduate Student, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Postgraduate Student, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Introduction: Achieving strong bond strength of adhesive systems to deep dentin is challenging. Various methods to improve bond strength to deep dentin have been investigated. Chlorhexidine (CHX) enhances the longevity of the bond between adhesives and dentin by inhibiting the collagenolytic activity of Matrix Metalloproteinases (MMPs). Benincasa hispida is a natural plant-based functional food containing minerals and vitamins that help prevent osteoporosis and improve bone health. Aim: To evaluate the Microshear Bond Strength (MSBS) of a 10-methacryloyloxydecyl-dihydrogen Phosphate-based (10-MDP) universal adhesive system to coronal dentin with different dentin surface pretreatments, specifically CHX and Benincasa hispida. Materials and Methods: An in-vitro pilot study was conducted at the Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India, from May 2023 to November 2023. Dentin discs (42) were prepared from human mandibular first molars. A standard etch-and-rinse protocol was performed on the discs. The specimens were assigned to different dentin pretreatments: Group-I (no pretreatment), Group-II (CHX) and Group-III (Benincasa hispida). CHX and Benincasa hispida were applied using micro applicator tips. A dentin bonding agent containing 10-MDP (DBA) was applied, photopolymerised and a composite restoration was fabricated. The MSBS to dentin was evaluated using a universal testing machine. The failure patterns were analysed using a stereomicroscope at 10x magnification. One-way Analysis of Variance (ANOVA) and Tukey’s Honestly Significant Difference (HSD) HSD tests were used for statistical analysis, with a significance level set at α=0.05. Results: The mean MSBS values were 24.2732±5.41329 MPa for Group-III, 14.3848±4.23492 MPa for Group-II and 6.9724±3.15837 MPa for Group-I. Pretreatment of dentin with B. hispida resulted in significantly higher MSBS values compared to the other two groups (p<0.001*). Conclusion: Pretreatment with B. hispida significantly improved MSBS. Thus, B. hispida pretreatment may be beneficial in increasing dentin bond strength.https://www.jcdr.net/articles/PDF/20224/73389_QC(AnK)_CE[Ra1]_F(SHU)_PF1(RI_SS)_PFA_NC(KM)_PN(KM).pdfdentin bondingdentine pretreatmenthybrid layermatrix metalloproteinase |
| spellingShingle | Girija S Sajjan Manda Sri Swathi P Arun Bhupathi Madhu Varma Kanumuri Rajulapati Kalyan Satish Rama Krishna Alla Tippireddy Srija Ch Uma Shalini Effect of Chlorhexidine and Benincasa Hispida Pretreatment on Microshear Bond Strength of Universal Adhesive System on Dentin: A Pilot Study Journal of Clinical and Diagnostic Research dentin bonding dentine pretreatment hybrid layer matrix metalloproteinase |
| title | Effect of Chlorhexidine and Benincasa Hispida Pretreatment on Microshear Bond Strength of Universal Adhesive System on Dentin: A Pilot Study |
| title_full | Effect of Chlorhexidine and Benincasa Hispida Pretreatment on Microshear Bond Strength of Universal Adhesive System on Dentin: A Pilot Study |
| title_fullStr | Effect of Chlorhexidine and Benincasa Hispida Pretreatment on Microshear Bond Strength of Universal Adhesive System on Dentin: A Pilot Study |
| title_full_unstemmed | Effect of Chlorhexidine and Benincasa Hispida Pretreatment on Microshear Bond Strength of Universal Adhesive System on Dentin: A Pilot Study |
| title_short | Effect of Chlorhexidine and Benincasa Hispida Pretreatment on Microshear Bond Strength of Universal Adhesive System on Dentin: A Pilot Study |
| title_sort | effect of chlorhexidine and benincasa hispida pretreatment on microshear bond strength of universal adhesive system on dentin a pilot study |
| topic | dentin bonding dentine pretreatment hybrid layer matrix metalloproteinase |
| url | https://www.jcdr.net/articles/PDF/20224/73389_QC(AnK)_CE[Ra1]_F(SHU)_PF1(RI_SS)_PFA_NC(KM)_PN(KM).pdf |
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