Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning
Background Aim. In case of high-dose acetaminophen intake, the active metabolite can not bind to the glutathione, thereby inducing cellular necrosis through binding to the cytosol proteins. This trial was performed to histologically and biochemically investigate whether leptin was protective against...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2015/357360 |
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| author | Murat Polat Serkan Cerrah Bulent Albayrak Serkan Ipek Mahmut Arabul Fatih Aslan Omer Yilmaz |
| author_facet | Murat Polat Serkan Cerrah Bulent Albayrak Serkan Ipek Mahmut Arabul Fatih Aslan Omer Yilmaz |
| author_sort | Murat Polat |
| collection | DOAJ |
| description | Background Aim. In case of high-dose acetaminophen intake, the active metabolite can not bind to the glutathione, thereby inducing cellular necrosis through binding to the cytosol proteins. This trial was performed to histologically and biochemically investigate whether leptin was protective against liver damage induced by paracetamol at toxic doses. Material and Method. In our trial, 30 female rats, divided into 5 groups, were used. IP leptin administration was performed after an hour in the group of rats, in which paracetamol poisoning was induced. The groups were as follows: Group 1: the control group, Group 2: 20 µg/kg leptin, Group 3: 2 g/kg paracetamol, Group 4: 2 g/kg paracetamol + 10 µg/kg leptin, and Group 5: 2 g/kg paracetamol + 20 µg/kg leptin. Results. The most significant increase was observed in the PARA 2 g/kg group, while the best improvement among the treatment groups occurred in the PARA 2 g/kg + LEP 10 µg/kg group (p < 0.05). While the most significant glutathione (GSH) reduction was observed in the PARA 2 g/kg group, the best improvement was in the PARA 2 g/kg + LEP 10 µg/kg group (p < 0.05). Conclusion. Liver damage occurring upon paracetamol poisoning manifests with hepatocyte breakdown occurring as a result of inflammation and oxidative stress. Leptin can prevent this damage thanks to its antioxidant and anti-inflammatory efficacy. |
| format | Article |
| id | doaj-art-ad8da2a58f604964a9d49a0593189e1a |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-ad8da2a58f604964a9d49a0593189e1a2025-08-20T03:39:41ZengWileyGastroenterology Research and Practice1687-61211687-630X2015-01-01201510.1155/2015/357360357360Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol PoisoningMurat Polat0Serkan Cerrah1Bulent Albayrak2Serkan Ipek3Mahmut Arabul4Fatih Aslan5Omer Yilmaz6Department of Internal Medicine, Erzurum Ataturk University, Yakutiye, 25240 Erzurum, TurkeyDepartment of Internal Medicine, Erzurum Ataturk University, Yakutiye, 25240 Erzurum, TurkeyDepartment of Internal Medicine, Erzurum Ataturk University, Yakutiye, 25240 Erzurum, TurkeyDepartment of Gastroenterology, Erzurum Ataturk University, Yakutiye, 25240 Erzurum, TurkeyDepartment of Gastroenterology, Erzurum Ataturk University, Yakutiye, 25240 Erzurum, TurkeyDepartment of Gastroenterology, Erzurum Ataturk University, Yakutiye, 25240 Erzurum, TurkeyDepartment of Gastroenterology, Atatürk Training and Research Hospital, İzmir Katip Çelebi University, Karabaglar, 35360 İzmir, TurkeyBackground Aim. In case of high-dose acetaminophen intake, the active metabolite can not bind to the glutathione, thereby inducing cellular necrosis through binding to the cytosol proteins. This trial was performed to histologically and biochemically investigate whether leptin was protective against liver damage induced by paracetamol at toxic doses. Material and Method. In our trial, 30 female rats, divided into 5 groups, were used. IP leptin administration was performed after an hour in the group of rats, in which paracetamol poisoning was induced. The groups were as follows: Group 1: the control group, Group 2: 20 µg/kg leptin, Group 3: 2 g/kg paracetamol, Group 4: 2 g/kg paracetamol + 10 µg/kg leptin, and Group 5: 2 g/kg paracetamol + 20 µg/kg leptin. Results. The most significant increase was observed in the PARA 2 g/kg group, while the best improvement among the treatment groups occurred in the PARA 2 g/kg + LEP 10 µg/kg group (p < 0.05). While the most significant glutathione (GSH) reduction was observed in the PARA 2 g/kg group, the best improvement was in the PARA 2 g/kg + LEP 10 µg/kg group (p < 0.05). Conclusion. Liver damage occurring upon paracetamol poisoning manifests with hepatocyte breakdown occurring as a result of inflammation and oxidative stress. Leptin can prevent this damage thanks to its antioxidant and anti-inflammatory efficacy.http://dx.doi.org/10.1155/2015/357360 |
| spellingShingle | Murat Polat Serkan Cerrah Bulent Albayrak Serkan Ipek Mahmut Arabul Fatih Aslan Omer Yilmaz Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning Gastroenterology Research and Practice |
| title | Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning |
| title_full | Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning |
| title_fullStr | Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning |
| title_full_unstemmed | Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning |
| title_short | Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning |
| title_sort | assessing the effect of leptin on liver damage in case of hepatic injury associated with paracetamol poisoning |
| url | http://dx.doi.org/10.1155/2015/357360 |
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