Antimicrobial susceptibility, virulence characteristics, and incidence of class 1 and 2 integrons in salmonella infantis isolated from clinical cases in broilers
Salmonella Infantis is a poultry-adapted Salmonella enterica serovar that is increasingly reported in broilers and is also regularly identified among human salmonellosis cases. This study aimed to investigate the presence and distribution of virulence determinants and their antimicrobial susceptibil...
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Kafkas University, Faculty of Veterinary Medicine
2024-12-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3161 |
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| author | Özkan ASLANTAŞ Süheyla TÜRKYILMAZ |
| author_facet | Özkan ASLANTAŞ Süheyla TÜRKYILMAZ |
| author_sort | Özkan ASLANTAŞ |
| collection | DOAJ |
| description | Salmonella Infantis is a poultry-adapted Salmonella enterica serovar that is increasingly
reported in broilers and is also regularly identified among human salmonellosis cases.
This study aimed to investigate the presence and distribution of virulence determinants
and their antimicrobial susceptibilities of S. Infantis isolates obtained from clinical cases
of broilers. In addition, selected 6 S. Infantis isolates were further characterized using
whole-genome sequencing (WGS). The majority of the isolates was resistant to at least
two or more antimicrobials. Only two isolates were susceptible to all antimicrobials
tested. Higher rates of resistance were observed against ciprofloxacin (96.4%),
tetracycline (96.4%), sulfamethoxazole-trimethoprim (76.8%), but low resistance rates
to chloramphenicol (8.9%), and ampicillin (8.9%) were detected. WGS analysis revealed
the presence of different resistome, but aac(6")-Iaa and tetA genes in all isolates, and
mutations in gyrA and parC genes playing a role in quinolone resistance. WGS also
revealed that all isolates were of sequence type 32 (ST32). Based on the presence of
virulence genes, the isolates were characterized into five virulence profile. Among the
examined virulence genes, invA, sopB, pipD, sifA, stn, spaN, slyA, and hilA were present
in all isolates. Only one isolate had all virulence genes examined. The findings of this
study provide valuable information on S. Infantis strains isolated from clinical cases of
broilers and current antimicrobial resistance levels and virulence determinants. High
resistance rates and the widespread occurrence of many virulence genes reveals that the
isolates have significant pathogenic potential and pose a threat to public health. |
| format | Article |
| id | doaj-art-ad878dfe173348d2bb79f06a0dc992eb |
| institution | Kabale University |
| issn | 1309-2251 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj-art-ad878dfe173348d2bb79f06a0dc992eb2025-01-04T21:15:10ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512024-12-01311919810.9775/kvfd.2024.329453161Antimicrobial susceptibility, virulence characteristics, and incidence of class 1 and 2 integrons in salmonella infantis isolated from clinical cases in broilersÖzkan ASLANTAŞ0Süheyla TÜRKYILMAZ1Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Microbiology, TR-31060 Hatay - TÜRKİYE Aydın Adnan Menderes University, Faculty of Veterinary Medicine, Department of Microbiology, TR-09010 Aydın - TÜRKİYESalmonella Infantis is a poultry-adapted Salmonella enterica serovar that is increasingly reported in broilers and is also regularly identified among human salmonellosis cases. This study aimed to investigate the presence and distribution of virulence determinants and their antimicrobial susceptibilities of S. Infantis isolates obtained from clinical cases of broilers. In addition, selected 6 S. Infantis isolates were further characterized using whole-genome sequencing (WGS). The majority of the isolates was resistant to at least two or more antimicrobials. Only two isolates were susceptible to all antimicrobials tested. Higher rates of resistance were observed against ciprofloxacin (96.4%), tetracycline (96.4%), sulfamethoxazole-trimethoprim (76.8%), but low resistance rates to chloramphenicol (8.9%), and ampicillin (8.9%) were detected. WGS analysis revealed the presence of different resistome, but aac(6")-Iaa and tetA genes in all isolates, and mutations in gyrA and parC genes playing a role in quinolone resistance. WGS also revealed that all isolates were of sequence type 32 (ST32). Based on the presence of virulence genes, the isolates were characterized into five virulence profile. Among the examined virulence genes, invA, sopB, pipD, sifA, stn, spaN, slyA, and hilA were present in all isolates. Only one isolate had all virulence genes examined. The findings of this study provide valuable information on S. Infantis strains isolated from clinical cases of broilers and current antimicrobial resistance levels and virulence determinants. High resistance rates and the widespread occurrence of many virulence genes reveals that the isolates have significant pathogenic potential and pose a threat to public health.https://vetdergikafkas.org/pdf.php?id=3161antimicrobial susceptibilityintegronsalmonella infantisvirulencewhole genome sequencing |
| spellingShingle | Özkan ASLANTAŞ Süheyla TÜRKYILMAZ Antimicrobial susceptibility, virulence characteristics, and incidence of class 1 and 2 integrons in salmonella infantis isolated from clinical cases in broilers Kafkas Universitesi Veteriner Fakültesi Dergisi antimicrobial susceptibility integron salmonella infantis virulence whole genome sequencing |
| title | Antimicrobial susceptibility, virulence characteristics, and incidence of class 1 and 2 integrons in salmonella infantis isolated from clinical cases in broilers |
| title_full | Antimicrobial susceptibility, virulence characteristics, and incidence of class 1 and 2 integrons in salmonella infantis isolated from clinical cases in broilers |
| title_fullStr | Antimicrobial susceptibility, virulence characteristics, and incidence of class 1 and 2 integrons in salmonella infantis isolated from clinical cases in broilers |
| title_full_unstemmed | Antimicrobial susceptibility, virulence characteristics, and incidence of class 1 and 2 integrons in salmonella infantis isolated from clinical cases in broilers |
| title_short | Antimicrobial susceptibility, virulence characteristics, and incidence of class 1 and 2 integrons in salmonella infantis isolated from clinical cases in broilers |
| title_sort | antimicrobial susceptibility virulence characteristics and incidence of class 1 and 2 integrons in salmonella infantis isolated from clinical cases in broilers |
| topic | antimicrobial susceptibility integron salmonella infantis virulence whole genome sequencing |
| url | https://vetdergikafkas.org/pdf.php?id=3161 |
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