Antimicrobial susceptibility, virulence characteristics, and incidence of class 1 and 2 integrons in salmonella infantis isolated from clinical cases in broilers
Salmonella Infantis is a poultry-adapted Salmonella enterica serovar that is increasingly reported in broilers and is also regularly identified among human salmonellosis cases. This study aimed to investigate the presence and distribution of virulence determinants and their antimicrobial susceptibil...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2024-12-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=3161 |
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| Summary: | Salmonella Infantis is a poultry-adapted Salmonella enterica serovar that is increasingly
reported in broilers and is also regularly identified among human salmonellosis cases.
This study aimed to investigate the presence and distribution of virulence determinants
and their antimicrobial susceptibilities of S. Infantis isolates obtained from clinical cases
of broilers. In addition, selected 6 S. Infantis isolates were further characterized using
whole-genome sequencing (WGS). The majority of the isolates was resistant to at least
two or more antimicrobials. Only two isolates were susceptible to all antimicrobials
tested. Higher rates of resistance were observed against ciprofloxacin (96.4%),
tetracycline (96.4%), sulfamethoxazole-trimethoprim (76.8%), but low resistance rates
to chloramphenicol (8.9%), and ampicillin (8.9%) were detected. WGS analysis revealed
the presence of different resistome, but aac(6")-Iaa and tetA genes in all isolates, and
mutations in gyrA and parC genes playing a role in quinolone resistance. WGS also
revealed that all isolates were of sequence type 32 (ST32). Based on the presence of
virulence genes, the isolates were characterized into five virulence profile. Among the
examined virulence genes, invA, sopB, pipD, sifA, stn, spaN, slyA, and hilA were present
in all isolates. Only one isolate had all virulence genes examined. The findings of this
study provide valuable information on S. Infantis strains isolated from clinical cases of
broilers and current antimicrobial resistance levels and virulence determinants. High
resistance rates and the widespread occurrence of many virulence genes reveals that the
isolates have significant pathogenic potential and pose a threat to public health. |
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| ISSN: | 1309-2251 |