High frequency of enterovirus serotype circulation in a densely populated area of India
Introduction: In the state of Uttar Pradesh in India, enteroviruses are a significant cause of infection presenting in endemic or epidemic forms. The present study aimed to use molecular methods to identify enterovirus serotypes in clinical specimens to determine their circulation in the community....
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2013-06-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/2413 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849323570412388352 |
|---|---|
| author | Arvind Kumar Deepti Shukla Shalini Srivastava Mohammad Z Idris Tapan N Dhole |
| author_facet | Arvind Kumar Deepti Shukla Shalini Srivastava Mohammad Z Idris Tapan N Dhole |
| author_sort | Arvind Kumar |
| collection | DOAJ |
| description |
Introduction: In the state of Uttar Pradesh in India, enteroviruses are a significant cause of infection presenting in endemic or epidemic forms. The present study aimed to use molecular methods to identify enterovirus serotypes in clinical specimens to determine their circulation in the community.
Methodology; A total of 320 clinical specimens were collected between January 2009 and December 2010 from children younger than 15 year of age in northern India. Reverse- transcription (RT) real time PCR and semi-nested RT PCR targeting the 5′untranslated region and VP1 region was used for the detection and identification of enterovirus serotypes.
Results: The enterovirus genome was detected in 79 (24.7%) of 320 clinical specimens by real time PCR. Central nervous system syndrome (CNS) was the most common clinical manifestation (n=32, 62.74%), followed by respiratory tract infection (n=8, 15.69%), acute febrile illness (n=7, 13.73%), and gastrointestinal disease (n=4, 7.84%). A total of 32 different serotypes were identified with the predominance of coxsackievirus B5 and echovirus 6. Phylogenetic analysis of partial VP1 gene sequences from this study showed that many enterovirus serotypes showed good similarity with strains from America and Europe in comparison to neighbouring Asian countries.
Conclusions: To our knowledge this is the first study of enterovirus prevalence from northern India based on unbiased molecular methods which leads to the identification of fifteen different enterovirus serotypes. The high frequency of enterovirus B species serotypes circulation may be an important cause of CNS infection in the children of this region.
|
| format | Article |
| id | doaj-art-ad8588b3d9da4376bd3bf325d3aebcc5 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2013-06-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-ad8588b3d9da4376bd3bf325d3aebcc52025-08-20T03:48:58ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802013-06-0170610.3855/jidc.2413High frequency of enterovirus serotype circulation in a densely populated area of IndiaArvind Kumar0Deepti Shukla1Shalini Srivastava2Mohammad Z Idris3Tapan N Dhole4Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, IndiaSanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, IndiaLucknow University, Lucknow, Uttar Pradesh, IndiaChhatrapati Shahuji Maharaj Medical University, Lucknow, Uttar Pradesh, IndiaSanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India Introduction: In the state of Uttar Pradesh in India, enteroviruses are a significant cause of infection presenting in endemic or epidemic forms. The present study aimed to use molecular methods to identify enterovirus serotypes in clinical specimens to determine their circulation in the community. Methodology; A total of 320 clinical specimens were collected between January 2009 and December 2010 from children younger than 15 year of age in northern India. Reverse- transcription (RT) real time PCR and semi-nested RT PCR targeting the 5′untranslated region and VP1 region was used for the detection and identification of enterovirus serotypes. Results: The enterovirus genome was detected in 79 (24.7%) of 320 clinical specimens by real time PCR. Central nervous system syndrome (CNS) was the most common clinical manifestation (n=32, 62.74%), followed by respiratory tract infection (n=8, 15.69%), acute febrile illness (n=7, 13.73%), and gastrointestinal disease (n=4, 7.84%). A total of 32 different serotypes were identified with the predominance of coxsackievirus B5 and echovirus 6. Phylogenetic analysis of partial VP1 gene sequences from this study showed that many enterovirus serotypes showed good similarity with strains from America and Europe in comparison to neighbouring Asian countries. Conclusions: To our knowledge this is the first study of enterovirus prevalence from northern India based on unbiased molecular methods which leads to the identification of fifteen different enterovirus serotypes. The high frequency of enterovirus B species serotypes circulation may be an important cause of CNS infection in the children of this region. https://jidc.org/index.php/journal/article/view/2413molecular identificationVP1human enterovirus Bphylogenetic analysis |
| spellingShingle | Arvind Kumar Deepti Shukla Shalini Srivastava Mohammad Z Idris Tapan N Dhole High frequency of enterovirus serotype circulation in a densely populated area of India Journal of Infection in Developing Countries molecular identification VP1 human enterovirus B phylogenetic analysis |
| title | High frequency of enterovirus serotype circulation in a densely populated area of India |
| title_full | High frequency of enterovirus serotype circulation in a densely populated area of India |
| title_fullStr | High frequency of enterovirus serotype circulation in a densely populated area of India |
| title_full_unstemmed | High frequency of enterovirus serotype circulation in a densely populated area of India |
| title_short | High frequency of enterovirus serotype circulation in a densely populated area of India |
| title_sort | high frequency of enterovirus serotype circulation in a densely populated area of india |
| topic | molecular identification VP1 human enterovirus B phylogenetic analysis |
| url | https://jidc.org/index.php/journal/article/view/2413 |
| work_keys_str_mv | AT arvindkumar highfrequencyofenterovirusserotypecirculationinadenselypopulatedareaofindia AT deeptishukla highfrequencyofenterovirusserotypecirculationinadenselypopulatedareaofindia AT shalinisrivastava highfrequencyofenterovirusserotypecirculationinadenselypopulatedareaofindia AT mohammadzidris highfrequencyofenterovirusserotypecirculationinadenselypopulatedareaofindia AT tapanndhole highfrequencyofenterovirusserotypecirculationinadenselypopulatedareaofindia |