qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (<i>Litsea coreana</i>)

qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (<i>Litsea coreana</i>)

To identify stable reference genes for qRT-PCR analysis across different developmental stages and color variations of tender leaves in <i>Litsea coreana</i>, seven candidate reference genes were selected based on existing transcriptome data. qRT-PCR was performed on tender leaves of <...

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Bibliographic Details
Main Authors: Qianli Dai, Min Lu, Ximeng Yang, Chenggong Lei, Feiyi Huang, Xueping Hu, Xin Huang, Xiaolong Nie, Daojing Chen, Sicheng Huang, Hengxing Zhu
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Current Issues in Molecular Biology
Subjects:
<i>Litsea coreana</i>
hawk tea
real-time quantitative PCR
reference gene
tender leaves discoloration
Online Access:https://www.mdpi.com/1467-3045/47/2/131
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Summary:To identify stable reference genes for qRT-PCR analysis across different developmental stages and color variations of tender leaves in <i>Litsea coreana</i>, seven candidate reference genes were selected based on existing transcriptome data. qRT-PCR was performed on tender leaves of <i>L. coreana</i> at various stages and under different color conditions. The stability of these genes was evaluated using GeNorm (version 2003), NormFinder (version 0953), BestKeeper (version 2003), and ReFinder software (version 2004). The most stable genes were selected, and the stability of the chosen reference genes was validated. <i>RPL</i> and <i>ACT</i> were the most stable genes across different leaf developmental stages, while <i>ACT</i> and <i>EF1-α</i> showed the highest stability across different leaf colors. Overall, <i>ACT</i> and <i>EF1-α</i> were the most stable reference genes for both developmental stages and color variations. <i>ACT</i> and <i>EF1-α</i> can be used as reliable reference genes for gene expression studies in the color change process of <i>L. coreana</i> tender leaves. This will provide a foundation for further research into the molecular mechanisms of leaf color changes and the development of color regulation genes in <i>L. coreana</i>.
ISSN:1467-3037
1467-3045

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