Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial
Background: Severe endometrial injury constitutes a significant risk to female fertility, often leading to the development of intrauterine adhesions. Pyroptosis, a form of programmed cell death associated with inflammation, is initiated by the cleavage of gasdermin family proteins...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IMR Press
2025-01-01
|
| Series: | Clinical and Experimental Obstetrics & Gynecology |
| Subjects: | |
| Online Access: | https://www.imrpress.com/journal/CEOG/52/1/10.31083/CEOG26554 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832586594586984448 |
|---|---|
| author | Haiyan Shan Mingyuan Xu Jie Ni Ling Zhou Mengchun Xue Jun Xue Xiaohuan Yuan Luyang Tao Mingyang Zhang Hong Zhang |
| author_facet | Haiyan Shan Mingyuan Xu Jie Ni Ling Zhou Mengchun Xue Jun Xue Xiaohuan Yuan Luyang Tao Mingyang Zhang Hong Zhang |
| author_sort | Haiyan Shan |
| collection | DOAJ |
| description | Background: Severe endometrial injury constitutes a significant risk to female fertility, often leading to the development of intrauterine adhesions. Pyroptosis, a form of programmed cell death associated with inflammation, is initiated by the cleavage of gasdermin family proteins by caspase, which has been implicated in endometrial injury. In recent years, hydrogen sulfide (H2S), a gaseous signaling molecule, exerts significant regulatory effects on pyroptosis in diverse pathological processes. The aim of this study is to elucidate the role of H2S in facilitating functional recovery of the endometrium following injury in a murine model. Methods: A prospective laboratory based randomized control trial was performed to evaluate the protective role of H2S in endometrial injury. Ethanol-induced endometrial injury mouse models were established and H2S donor sodium hydrosulfide (NaHS) was randomly administered to the injured mice. Western blot analysis was conducted to assess changes in the expression of endogenous H2S metabolism and pyroptosis-related markers and histological analysis (Hematoxylin & Eosin and Masson staining) was employed to examine alterations in endometrial morphology. Finally, a fertility test was performed to evaluate the restoration of the uterine function. Results: Following endometrial injury, the expression of key endogenous H2S enzymes-cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (MST)-is significantly reduced, while the levels of pyroptosis-related proteins are elevated (p < 0.05). However, treatment with H2S resulted in an increase in the expression of endogenous H2S enzymes and a decrease in pyroptosis-associated proteins compared to the Model group (p < 0.05). Moreover, endometrial morphology and embryo count showed the most pronounced improvement in the H2S treatment group (p < 0.05). Conclusions: This study confirms the therapeutic efficacy of H2S in facilitating the recovery of injured endometrium and revealed its potential therapeutic mechanism, offering a promising therapeutic avenue for patients with severe endometrial injury. |
| format | Article |
| id | doaj-art-ad791a2121b64528a263cfdb3ac61c29 |
| institution | Kabale University |
| issn | 0390-6663 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj-art-ad791a2121b64528a263cfdb3ac61c292025-01-25T07:14:26ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632025-01-015212655410.31083/CEOG26554S0390-6663(24)02544-2Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control TrialHaiyan Shan0Mingyuan Xu1Jie Ni2Ling Zhou3Mengchun Xue4Jun Xue5Xiaohuan Yuan6Luyang Tao7Mingyang Zhang8Hong Zhang9Department of Obstetrics and Gynecology, Second Affiliated Hospital of Soochow University, 215123 Suzhou, Jiangsu, ChinaDepartment of Forensic Medicine, School of Basic Medicine, Suzhou Medical College of Soochow University, 215123 Suzhou, Jiangsu, ChinaDepartment of Obstetrics and Gynecology, Second Affiliated Hospital of Soochow University, 215123 Suzhou, Jiangsu, ChinaDepartment of Obstetrics and Gynecology, Second Affiliated Hospital of Soochow University, 215123 Suzhou, Jiangsu, ChinaCollege of Life Sciences, Mudanjiang Medical University, 157011 Mudanjiang, Heilongjiang, ChinaCollege of Life Sciences, Mudanjiang Medical University, 157011 Mudanjiang, Heilongjiang, ChinaCollege of Life Sciences, Mudanjiang Medical University, 157011 Mudanjiang, Heilongjiang, ChinaDepartment of Forensic Medicine, School of Basic Medicine, Suzhou Medical College of Soochow University, 215123 Suzhou, Jiangsu, ChinaDepartment of Forensic Medicine, School of Basic Medicine, Suzhou Medical College of Soochow University, 215123 Suzhou, Jiangsu, ChinaDepartment of Obstetrics and Gynecology, Second Affiliated Hospital of Soochow University, 215123 Suzhou, Jiangsu, ChinaBackground: Severe endometrial injury constitutes a significant risk to female fertility, often leading to the development of intrauterine adhesions. Pyroptosis, a form of programmed cell death associated with inflammation, is initiated by the cleavage of gasdermin family proteins by caspase, which has been implicated in endometrial injury. In recent years, hydrogen sulfide (H2S), a gaseous signaling molecule, exerts significant regulatory effects on pyroptosis in diverse pathological processes. The aim of this study is to elucidate the role of H2S in facilitating functional recovery of the endometrium following injury in a murine model. Methods: A prospective laboratory based randomized control trial was performed to evaluate the protective role of H2S in endometrial injury. Ethanol-induced endometrial injury mouse models were established and H2S donor sodium hydrosulfide (NaHS) was randomly administered to the injured mice. Western blot analysis was conducted to assess changes in the expression of endogenous H2S metabolism and pyroptosis-related markers and histological analysis (Hematoxylin & Eosin and Masson staining) was employed to examine alterations in endometrial morphology. Finally, a fertility test was performed to evaluate the restoration of the uterine function. Results: Following endometrial injury, the expression of key endogenous H2S enzymes-cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (MST)-is significantly reduced, while the levels of pyroptosis-related proteins are elevated (p < 0.05). However, treatment with H2S resulted in an increase in the expression of endogenous H2S enzymes and a decrease in pyroptosis-associated proteins compared to the Model group (p < 0.05). Moreover, endometrial morphology and embryo count showed the most pronounced improvement in the H2S treatment group (p < 0.05). Conclusions: This study confirms the therapeutic efficacy of H2S in facilitating the recovery of injured endometrium and revealed its potential therapeutic mechanism, offering a promising therapeutic avenue for patients with severe endometrial injury.https://www.imrpress.com/journal/CEOG/52/1/10.31083/CEOG26554endometrial injuryhydrogen sulfideintrauterine adhesionspyroptosis |
| spellingShingle | Haiyan Shan Mingyuan Xu Jie Ni Ling Zhou Mengchun Xue Jun Xue Xiaohuan Yuan Luyang Tao Mingyang Zhang Hong Zhang Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial Clinical and Experimental Obstetrics & Gynecology endometrial injury hydrogen sulfide intrauterine adhesions pyroptosis |
| title | Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial |
| title_full | Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial |
| title_fullStr | Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial |
| title_full_unstemmed | Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial |
| title_short | Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial |
| title_sort | hydrogen sulfide promotes functional endometrium recovery via regulating pyroptosis in severe endometrial injury a prospective laboratory based randomized control trial |
| topic | endometrial injury hydrogen sulfide intrauterine adhesions pyroptosis |
| url | https://www.imrpress.com/journal/CEOG/52/1/10.31083/CEOG26554 |
| work_keys_str_mv | AT haiyanshan hydrogensulfidepromotesfunctionalendometriumrecoveryviaregulatingpyroptosisinsevereendometrialinjuryaprospectivelaboratorybasedrandomizedcontroltrial AT mingyuanxu hydrogensulfidepromotesfunctionalendometriumrecoveryviaregulatingpyroptosisinsevereendometrialinjuryaprospectivelaboratorybasedrandomizedcontroltrial AT jieni hydrogensulfidepromotesfunctionalendometriumrecoveryviaregulatingpyroptosisinsevereendometrialinjuryaprospectivelaboratorybasedrandomizedcontroltrial AT lingzhou hydrogensulfidepromotesfunctionalendometriumrecoveryviaregulatingpyroptosisinsevereendometrialinjuryaprospectivelaboratorybasedrandomizedcontroltrial AT mengchunxue hydrogensulfidepromotesfunctionalendometriumrecoveryviaregulatingpyroptosisinsevereendometrialinjuryaprospectivelaboratorybasedrandomizedcontroltrial AT junxue hydrogensulfidepromotesfunctionalendometriumrecoveryviaregulatingpyroptosisinsevereendometrialinjuryaprospectivelaboratorybasedrandomizedcontroltrial AT xiaohuanyuan hydrogensulfidepromotesfunctionalendometriumrecoveryviaregulatingpyroptosisinsevereendometrialinjuryaprospectivelaboratorybasedrandomizedcontroltrial AT luyangtao hydrogensulfidepromotesfunctionalendometriumrecoveryviaregulatingpyroptosisinsevereendometrialinjuryaprospectivelaboratorybasedrandomizedcontroltrial AT mingyangzhang hydrogensulfidepromotesfunctionalendometriumrecoveryviaregulatingpyroptosisinsevereendometrialinjuryaprospectivelaboratorybasedrandomizedcontroltrial AT hongzhang hydrogensulfidepromotesfunctionalendometriumrecoveryviaregulatingpyroptosisinsevereendometrialinjuryaprospectivelaboratorybasedrandomizedcontroltrial |