Study of the influence of various physiologically active substances on changes in the lipid composition and phospholipase activity of damaged somatic nerves
The changes in lipid composition and phospholipase activity in damaged somatic nerves were studied against the background of the action of potassium hyaluronate and insulin-like growth factor-1. It has been shown that nerve cutting is accompanied by an increase in phospholipase A2 activity, resultin...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Saratov State University
2024-12-01
|
| Series: | Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология |
| Subjects: | |
| Online Access: | https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2024/12/himiya_2024_4-448-460.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850119643033763840 |
|---|---|
| author | Revin, Viktor V. Parchaykina, Marina V. Chudaikina, Elena V. Revina, Elvira S. Molchanov, Ivan D. Simakova, Milena A. Zavarykina, Anastasia Vyacheslavovna Grunyushkin, Igor P. Devyatkin, Arkadiy А. |
| author_facet | Revin, Viktor V. Parchaykina, Marina V. Chudaikina, Elena V. Revina, Elvira S. Molchanov, Ivan D. Simakova, Milena A. Zavarykina, Anastasia Vyacheslavovna Grunyushkin, Igor P. Devyatkin, Arkadiy А. |
| author_sort | Revin, Viktor V. |
| collection | DOAJ |
| description | The changes in lipid composition and phospholipase activity in damaged somatic nerves were studied against the background of the action of potassium hyaluronate and insulin-like growth factor-1. It has been shown that nerve cutting is accompanied by an increase in phospholipase A2 activity, resulting in an accumulation of lysophospholipids and free fatty acids, as well as an increase in phosphatidylinositol levels and a decrease in diacylglycerol content, which is most likely due to inactivation of phosphoinositide-specifi c phospholipase C against the background of injury to the nerve conductor. The introduction of potassium hyaluronate and insulin-like growth factor-1 enhances the recovery processes in the injured nerve conductor, however, the mechanisms of their action remain diff erent. According to the literature data and the results of our own research, the action of potassium hyaluronate and insulin-like growth factor-1 is realized as a result of the launch of signaling pathways associated with the regulation of the activity of enzymes from the phospholipase family. At the same time, our data on a decrease in the activity of phospholipase A2 and the absence of signifi cant changes in the level of phosphatidylinositol and diacylglycerol indicate that potassium hyaluronate most likely exerts its eff ect through the PL A2 -mediated pathway. In addition, it was shown that against the background of the action of IGF-1, an intensifi cation of phosphoinositide metabolism is observed, which is explained by the activation of phosphoinositidespecifi c phospholipase C. According to the literature, the launch of the phospholipase C-mediated mechanism is accompanied by the formation of components of the phosphatidylinositol 3-kinase signaling pathway involved in stimulation of the expression of various transcription factors necessary for axonal regeneration and restoration of the functioning of injured nerve conductors. |
| format | Article |
| id | doaj-art-ad7318ba429d40a4aed40fc717310369 |
| institution | OA Journals |
| issn | 1816-9775 2541-8971 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Saratov State University |
| record_format | Article |
| series | Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология |
| spelling | doaj-art-ad7318ba429d40a4aed40fc7173103692025-08-20T02:35:35ZengSaratov State UniversityИзвестия Саратовского университета. Новая серия: Серия Химия. Биология. Экология1816-97752541-89712024-12-0124444846010.18500/1816-9775-2024-24-4-448-460Study of the influence of various physiologically active substances on changes in the lipid composition and phospholipase activity of damaged somatic nervesRevin, Viktor V.0Parchaykina, Marina V.1Chudaikina, Elena V.2Revina, Elvira S.3Molchanov, Ivan D.4Simakova, Milena A.5Zavarykina, Anastasia Vyacheslavovna6Grunyushkin, Igor P.7Devyatkin, Arkadiy А.8Ogarev Mordovia State University, 68, Bolshevistskaya Str., Saransk, 430005, RussiaOgarev Mordovia State University, 68, Bolshevistskaya Str., Saransk, 430005, RussiaOgarev Mordovia State University, 68, Bolshevistskaya Str., Saransk, 430005, RussiaOgarev Mordovia State University, 68, Bolshevistskaya Str., Saransk, 430005, RussiaOgarev Mordovia State University, 68, Bolshevistskaya Str., Saransk, 430005, RussiaOgarev Mordovia State University, 68, Bolshevistskaya Str., Saransk, 430005, RussiaOgarev Mordovia State University, 430005 Saransk, RussiaOgarev Mordovia State University, 430005 Saransk, RussiaOgarev Mordovia State University, 430005 Saransk, RussiaThe changes in lipid composition and phospholipase activity in damaged somatic nerves were studied against the background of the action of potassium hyaluronate and insulin-like growth factor-1. It has been shown that nerve cutting is accompanied by an increase in phospholipase A2 activity, resulting in an accumulation of lysophospholipids and free fatty acids, as well as an increase in phosphatidylinositol levels and a decrease in diacylglycerol content, which is most likely due to inactivation of phosphoinositide-specifi c phospholipase C against the background of injury to the nerve conductor. The introduction of potassium hyaluronate and insulin-like growth factor-1 enhances the recovery processes in the injured nerve conductor, however, the mechanisms of their action remain diff erent. According to the literature data and the results of our own research, the action of potassium hyaluronate and insulin-like growth factor-1 is realized as a result of the launch of signaling pathways associated with the regulation of the activity of enzymes from the phospholipase family. At the same time, our data on a decrease in the activity of phospholipase A2 and the absence of signifi cant changes in the level of phosphatidylinositol and diacylglycerol indicate that potassium hyaluronate most likely exerts its eff ect through the PL A2 -mediated pathway. In addition, it was shown that against the background of the action of IGF-1, an intensifi cation of phosphoinositide metabolism is observed, which is explained by the activation of phosphoinositidespecifi c phospholipase C. According to the literature, the launch of the phospholipase C-mediated mechanism is accompanied by the formation of components of the phosphatidylinositol 3-kinase signaling pathway involved in stimulation of the expression of various transcription factors necessary for axonal regeneration and restoration of the functioning of injured nerve conductors.https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2024/12/himiya_2024_4-448-460.pdflysophospholipidsfree fatty acidspotassium hyaluronateinsulin-like growth factor-1regenerationsomatic nervesphospholipase activity |
| spellingShingle | Revin, Viktor V. Parchaykina, Marina V. Chudaikina, Elena V. Revina, Elvira S. Molchanov, Ivan D. Simakova, Milena A. Zavarykina, Anastasia Vyacheslavovna Grunyushkin, Igor P. Devyatkin, Arkadiy А. Study of the influence of various physiologically active substances on changes in the lipid composition and phospholipase activity of damaged somatic nerves Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология lysophospholipids free fatty acids potassium hyaluronate insulin-like growth factor-1 regeneration somatic nerves phospholipase activity |
| title | Study of the influence of various physiologically active substances on changes in the lipid composition and phospholipase activity of damaged somatic nerves |
| title_full | Study of the influence of various physiologically active substances on changes in the lipid composition and phospholipase activity of damaged somatic nerves |
| title_fullStr | Study of the influence of various physiologically active substances on changes in the lipid composition and phospholipase activity of damaged somatic nerves |
| title_full_unstemmed | Study of the influence of various physiologically active substances on changes in the lipid composition and phospholipase activity of damaged somatic nerves |
| title_short | Study of the influence of various physiologically active substances on changes in the lipid composition and phospholipase activity of damaged somatic nerves |
| title_sort | study of the influence of various physiologically active substances on changes in the lipid composition and phospholipase activity of damaged somatic nerves |
| topic | lysophospholipids free fatty acids potassium hyaluronate insulin-like growth factor-1 regeneration somatic nerves phospholipase activity |
| url | https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2024/12/himiya_2024_4-448-460.pdf |
| work_keys_str_mv | AT revinviktorv studyoftheinfluenceofvariousphysiologicallyactivesubstancesonchangesinthelipidcompositionandphospholipaseactivityofdamagedsomaticnerves AT parchaykinamarinav studyoftheinfluenceofvariousphysiologicallyactivesubstancesonchangesinthelipidcompositionandphospholipaseactivityofdamagedsomaticnerves AT chudaikinaelenav studyoftheinfluenceofvariousphysiologicallyactivesubstancesonchangesinthelipidcompositionandphospholipaseactivityofdamagedsomaticnerves AT revinaelviras studyoftheinfluenceofvariousphysiologicallyactivesubstancesonchangesinthelipidcompositionandphospholipaseactivityofdamagedsomaticnerves AT molchanovivand studyoftheinfluenceofvariousphysiologicallyactivesubstancesonchangesinthelipidcompositionandphospholipaseactivityofdamagedsomaticnerves AT simakovamilenaa studyoftheinfluenceofvariousphysiologicallyactivesubstancesonchangesinthelipidcompositionandphospholipaseactivityofdamagedsomaticnerves AT zavarykinaanastasiavyacheslavovna studyoftheinfluenceofvariousphysiologicallyactivesubstancesonchangesinthelipidcompositionandphospholipaseactivityofdamagedsomaticnerves AT grunyushkinigorp studyoftheinfluenceofvariousphysiologicallyactivesubstancesonchangesinthelipidcompositionandphospholipaseactivityofdamagedsomaticnerves AT devyatkinarkadiya studyoftheinfluenceofvariousphysiologicallyactivesubstancesonchangesinthelipidcompositionandphospholipaseactivityofdamagedsomaticnerves |