Detection of Quadruplex DNA by Gold Nanoparticles
Gold nanoparticles have been used as a probe to detect low (<10 ppb) concentrations of quadruplex DNA. These nanoparticles display a tendency to form aggregates in the presence of certain quadruplex forms, as observed via enhanced plasmon resonance light scattering (PRLS) signals. These nanoparti...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/327603 |
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| _version_ | 1832547595303518208 |
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| author | Heather F. Crouse Alex Doudt Cassie Zerbe Swarna Basu |
| author_facet | Heather F. Crouse Alex Doudt Cassie Zerbe Swarna Basu |
| author_sort | Heather F. Crouse |
| collection | DOAJ |
| description | Gold nanoparticles have been used as a probe to detect low (<10 ppb) concentrations of quadruplex DNA. These nanoparticles display a tendency to form aggregates in the presence of certain quadruplex forms, as observed via enhanced plasmon resonance light scattering (PRLS) signals. These nanoparticles showed differing degrees of interactions with different types of quadruplex and mixed sequences but no interaction with duplex DNA. Enhancement of PRLS signals greater than 50% was observed at nanomolar DNA concentration, and a lower limit of detection of 2.1 nM was established for three different quadruplex DNA sequences, including the thrombin-inhibiting single-stranded 15 mer aptamer DNA, d(GGTTGGTGTGGTTGG), and the double-stranded 12 mer DNA, d(G4T4G4). Two different sample preparation protocols were used for the PRLS experiments, and they yielded similar results. |
| format | Article |
| id | doaj-art-ad53d4b237c74e58b662cbecb5de817c |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-ad53d4b237c74e58b662cbecb5de817c2025-02-03T06:44:18ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732012-01-01201210.1155/2012/327603327603Detection of Quadruplex DNA by Gold NanoparticlesHeather F. Crouse0Alex Doudt1Cassie Zerbe2Swarna Basu3Department of Chemistry, Susquehanna University, 514 University Avenue, Selinsgrove, PA 17870, USADepartment of Chemistry, Susquehanna University, 514 University Avenue, Selinsgrove, PA 17870, USADepartment of Chemistry, Susquehanna University, 514 University Avenue, Selinsgrove, PA 17870, USADepartment of Chemistry, Susquehanna University, 514 University Avenue, Selinsgrove, PA 17870, USAGold nanoparticles have been used as a probe to detect low (<10 ppb) concentrations of quadruplex DNA. These nanoparticles display a tendency to form aggregates in the presence of certain quadruplex forms, as observed via enhanced plasmon resonance light scattering (PRLS) signals. These nanoparticles showed differing degrees of interactions with different types of quadruplex and mixed sequences but no interaction with duplex DNA. Enhancement of PRLS signals greater than 50% was observed at nanomolar DNA concentration, and a lower limit of detection of 2.1 nM was established for three different quadruplex DNA sequences, including the thrombin-inhibiting single-stranded 15 mer aptamer DNA, d(GGTTGGTGTGGTTGG), and the double-stranded 12 mer DNA, d(G4T4G4). Two different sample preparation protocols were used for the PRLS experiments, and they yielded similar results.http://dx.doi.org/10.1155/2012/327603 |
| spellingShingle | Heather F. Crouse Alex Doudt Cassie Zerbe Swarna Basu Detection of Quadruplex DNA by Gold Nanoparticles Journal of Analytical Methods in Chemistry |
| title | Detection of Quadruplex DNA by Gold Nanoparticles |
| title_full | Detection of Quadruplex DNA by Gold Nanoparticles |
| title_fullStr | Detection of Quadruplex DNA by Gold Nanoparticles |
| title_full_unstemmed | Detection of Quadruplex DNA by Gold Nanoparticles |
| title_short | Detection of Quadruplex DNA by Gold Nanoparticles |
| title_sort | detection of quadruplex dna by gold nanoparticles |
| url | http://dx.doi.org/10.1155/2012/327603 |
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