Lactobacillus brevis-Derived Exosomes Enhance Skin Barrier Integrity by Upregulating Key Barrier-Related Proteins
Yong-Han Cho,1 Ji-Woo Kim,1 Nari Kim,1 Hee-Sik Kim,1 Jun-Hwan Jang,1 Jun-Tae Bae,1 Wanil Kim2 1J2K-Metabiome, J2KBIO, Cheongju, Republic of Korea; 2Department of Biochemistry, Department of Convergence Medical Science, and Institute of Medical Science, Gyeongsang National University College of Medic...
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Dove Medical Press
2025-05-01
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| Series: | Clinical, Cosmetic and Investigational Dermatology |
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| Online Access: | https://www.dovepress.com/lactobacillus-brevis-derived-exosomes-enhance-skin-barrier-integrity-b-peer-reviewed-fulltext-article-CCID |
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| author | Cho YH Kim JW Kim N Kim HS Jang JH Bae JT Kim W |
| author_facet | Cho YH Kim JW Kim N Kim HS Jang JH Bae JT Kim W |
| author_sort | Cho YH |
| collection | DOAJ |
| description | Yong-Han Cho,1 Ji-Woo Kim,1 Nari Kim,1 Hee-Sik Kim,1 Jun-Hwan Jang,1 Jun-Tae Bae,1 Wanil Kim2 1J2K-Metabiome, J2KBIO, Cheongju, Republic of Korea; 2Department of Biochemistry, Department of Convergence Medical Science, and Institute of Medical Science, Gyeongsang National University College of Medicine, Jinju, Republic of KoreaCorrespondence: Wanil Kim, Email wkim@gnu.ac.krIntroduction: The human skin, comprising the epidermis, dermis, and subcutaneous fat layers, serves as a critical barrier against external stimuli. The integrity of this barrier function is essential for preventing skin damage and diseases. When compromised, it can lead to various dermatological issues.Methods: This study investigated the efficacy of Lactobacillus brevis J2K55-derived exosomes (LBDEs) on enhancing skin barrier function. High-purity LBDEs were produced and characterized using nanoparticle tracking analysis and Cryo-TEM, concentrated to 1.52× 108 particles/mL with sizes ranging from 50 to 200 nm. The LBDEs were then applied to human keratinocytes, HaCaT cells, and a live human skin model to analyze the expression of genes significant to skin barrier function.Results: In vitro experiments demonstrated that 2.5% LBDEs increased Filaggrin mRNA expression by 301.80% compared to the control. In an ex vivo skin damage model induced by physical stimulation and UVB (Ultraviolet B) irradiation, 1% LBDEs treatment significantly upregulated the expression of key barrier-related proteins, including Aquaporin-3 (180.8%), Claudin-1 (205.4%), Filaggrin (309.9%), Loricrin (365.2%), and Serine palmitoyltransferase (191.3%), in comparison to the friction and UVB-induced control group.Conclusion: These results suggest that LBDEs have potential in enhancing skin barrier function, as evidenced by increased expression of crucial barrier-related proteins in both in vitro and ex vivo models. Keywords: exosome, skin barrier, lactobacillus brevis, nanoparticle tracking analysis, Cryo-TEM |
| format | Article |
| id | doaj-art-ad4cbc204e614c868bfacfc422506969 |
| institution | OA Journals |
| issn | 1178-7015 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical, Cosmetic and Investigational Dermatology |
| spelling | doaj-art-ad4cbc204e614c868bfacfc4225069692025-08-20T02:15:29ZengDove Medical PressClinical, Cosmetic and Investigational Dermatology1178-70152025-05-01Volume 18Issue 111511162102806Lactobacillus brevis-Derived Exosomes Enhance Skin Barrier Integrity by Upregulating Key Barrier-Related ProteinsCho YH0Kim JW1Kim N2Kim HS3Jang JH4Bae JT5Kim W6laboratorylaboratoryJ2K METABIOMEJ2K MetabiomelaboratoryJ2KBIOCollege of MedicineYong-Han Cho,1 Ji-Woo Kim,1 Nari Kim,1 Hee-Sik Kim,1 Jun-Hwan Jang,1 Jun-Tae Bae,1 Wanil Kim2 1J2K-Metabiome, J2KBIO, Cheongju, Republic of Korea; 2Department of Biochemistry, Department of Convergence Medical Science, and Institute of Medical Science, Gyeongsang National University College of Medicine, Jinju, Republic of KoreaCorrespondence: Wanil Kim, Email wkim@gnu.ac.krIntroduction: The human skin, comprising the epidermis, dermis, and subcutaneous fat layers, serves as a critical barrier against external stimuli. The integrity of this barrier function is essential for preventing skin damage and diseases. When compromised, it can lead to various dermatological issues.Methods: This study investigated the efficacy of Lactobacillus brevis J2K55-derived exosomes (LBDEs) on enhancing skin barrier function. High-purity LBDEs were produced and characterized using nanoparticle tracking analysis and Cryo-TEM, concentrated to 1.52× 108 particles/mL with sizes ranging from 50 to 200 nm. The LBDEs were then applied to human keratinocytes, HaCaT cells, and a live human skin model to analyze the expression of genes significant to skin barrier function.Results: In vitro experiments demonstrated that 2.5% LBDEs increased Filaggrin mRNA expression by 301.80% compared to the control. In an ex vivo skin damage model induced by physical stimulation and UVB (Ultraviolet B) irradiation, 1% LBDEs treatment significantly upregulated the expression of key barrier-related proteins, including Aquaporin-3 (180.8%), Claudin-1 (205.4%), Filaggrin (309.9%), Loricrin (365.2%), and Serine palmitoyltransferase (191.3%), in comparison to the friction and UVB-induced control group.Conclusion: These results suggest that LBDEs have potential in enhancing skin barrier function, as evidenced by increased expression of crucial barrier-related proteins in both in vitro and ex vivo models. Keywords: exosome, skin barrier, lactobacillus brevis, nanoparticle tracking analysis, Cryo-TEMhttps://www.dovepress.com/lactobacillus-brevis-derived-exosomes-enhance-skin-barrier-integrity-b-peer-reviewed-fulltext-article-CCIDskin barrierLactobacillus brevisNanoparticle tracking analysisCryo-TEM |
| spellingShingle | Cho YH Kim JW Kim N Kim HS Jang JH Bae JT Kim W Lactobacillus brevis-Derived Exosomes Enhance Skin Barrier Integrity by Upregulating Key Barrier-Related Proteins Clinical, Cosmetic and Investigational Dermatology skin barrier Lactobacillus brevis Nanoparticle tracking analysis Cryo-TEM |
| title | Lactobacillus brevis-Derived Exosomes Enhance Skin Barrier Integrity by Upregulating Key Barrier-Related Proteins |
| title_full | Lactobacillus brevis-Derived Exosomes Enhance Skin Barrier Integrity by Upregulating Key Barrier-Related Proteins |
| title_fullStr | Lactobacillus brevis-Derived Exosomes Enhance Skin Barrier Integrity by Upregulating Key Barrier-Related Proteins |
| title_full_unstemmed | Lactobacillus brevis-Derived Exosomes Enhance Skin Barrier Integrity by Upregulating Key Barrier-Related Proteins |
| title_short | Lactobacillus brevis-Derived Exosomes Enhance Skin Barrier Integrity by Upregulating Key Barrier-Related Proteins |
| title_sort | lactobacillus brevis derived exosomes enhance skin barrier integrity by upregulating key barrier related proteins |
| topic | skin barrier Lactobacillus brevis Nanoparticle tracking analysis Cryo-TEM |
| url | https://www.dovepress.com/lactobacillus-brevis-derived-exosomes-enhance-skin-barrier-integrity-b-peer-reviewed-fulltext-article-CCID |
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