Polyacrylic acid-coated iron oxide nanoparticles could be a useful tool for tracking inflammatory monocytes
Aim: To establish the effect of poly(acrylic acid)-coated iron oxide nanoparticles (PAC-IONs) and later exposure to a magnetic field on the differentiation of mononuclear phagocytes into macrophages. Methods: By flow cytometry, cell death was evaluated with DIOC6 and PI, Poly (ADP-ribose) Polymerase...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-12-01
|
| Series: | Future Science OA |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/fsoa-2019-0066 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850153130549837824 |
|---|---|
| author | Manuela Giraldo-Villegas Jeaneth Urquijo Oscar L Arnache-Olmos Mauricio Rojas-López |
| author_facet | Manuela Giraldo-Villegas Jeaneth Urquijo Oscar L Arnache-Olmos Mauricio Rojas-López |
| author_sort | Manuela Giraldo-Villegas |
| collection | DOAJ |
| description | Aim: To establish the effect of poly(acrylic acid)-coated iron oxide nanoparticles (PAC-IONs) and later exposure to a magnetic field on the differentiation of mononuclear phagocytes into macrophages. Methods: By flow cytometry, cell death was evaluated with DIOC6 and PI, Poly (ADP-ribose) Polymerases (PARP) fragmentation, H2AX phosphorylation and TUNEL assay. Cytokines by Cytokine bead array and the intracellular amount of iron by atomic absorption spectrometry. Results: PAC-IONs did not induce apoptosis, modify the cell membrane integrity or alter the mitochondrial membrane potential. They did not affect the cell morphology, the pattern of cytokine accumulation or the activating role of differentiation of mononuclear phagocytes into macrophages on the proliferation of autologous T cells. Conclusion: This evidence indicates that the PAC-IONs are safe and biocompatible. Moreover, the selectivity of the PAC-IONs for mononuclear phagocytes, as well as their increased uptake by non-classical monocytes, warrant future research with a view to their use as a contrast agent, a useful tool for in vivo tracking of tissue-infiltrating mononuclear phagocytes. |
| format | Article |
| id | doaj-art-ad48506515f94e00a665653de76fec98 |
| institution | OA Journals |
| issn | 2056-5623 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Future Science OA |
| spelling | doaj-art-ad48506515f94e00a665653de76fec982025-08-20T02:25:48ZengTaylor & Francis GroupFuture Science OA2056-56232019-12-0151010.2144/fsoa-2019-0066Polyacrylic acid-coated iron oxide nanoparticles could be a useful tool for tracking inflammatory monocytesManuela Giraldo-Villegas0Jeaneth Urquijo1Oscar L Arnache-Olmos2Mauricio Rojas-López31Grupo de Inmunología Celular e Inmunogenética, Sede de Investigación Universitaria (SIU), Universidad de Antioquia (UDEA), Calle 70 No. 52-21, Medellín, Colombia2Grupo de Física del Estado Sólido, Sede de Investigación Universitaria (SIU), Universidad de Antioquia (UDEA), Medellín, Colombia2Grupo de Física del Estado Sólido, Sede de Investigación Universitaria (SIU), Universidad de Antioquia (UDEA), Medellín, Colombia1Grupo de Inmunología Celular e Inmunogenética, Sede de Investigación Universitaria (SIU), Universidad de Antioquia (UDEA), Calle 70 No. 52-21, Medellín, ColombiaAim: To establish the effect of poly(acrylic acid)-coated iron oxide nanoparticles (PAC-IONs) and later exposure to a magnetic field on the differentiation of mononuclear phagocytes into macrophages. Methods: By flow cytometry, cell death was evaluated with DIOC6 and PI, Poly (ADP-ribose) Polymerases (PARP) fragmentation, H2AX phosphorylation and TUNEL assay. Cytokines by Cytokine bead array and the intracellular amount of iron by atomic absorption spectrometry. Results: PAC-IONs did not induce apoptosis, modify the cell membrane integrity or alter the mitochondrial membrane potential. They did not affect the cell morphology, the pattern of cytokine accumulation or the activating role of differentiation of mononuclear phagocytes into macrophages on the proliferation of autologous T cells. Conclusion: This evidence indicates that the PAC-IONs are safe and biocompatible. Moreover, the selectivity of the PAC-IONs for mononuclear phagocytes, as well as their increased uptake by non-classical monocytes, warrant future research with a view to their use as a contrast agent, a useful tool for in vivo tracking of tissue-infiltrating mononuclear phagocytes.https://www.future-science.com/doi/10.2144/fsoa-2019-0066cytokinesinflammationiron oxide nanoparticlesmonocyte-derived macrophagesmonocytesmononuclear phagocytes |
| spellingShingle | Manuela Giraldo-Villegas Jeaneth Urquijo Oscar L Arnache-Olmos Mauricio Rojas-López Polyacrylic acid-coated iron oxide nanoparticles could be a useful tool for tracking inflammatory monocytes Future Science OA cytokines inflammation iron oxide nanoparticles monocyte-derived macrophages monocytes mononuclear phagocytes |
| title | Polyacrylic acid-coated iron oxide nanoparticles could be a useful tool for tracking inflammatory monocytes |
| title_full | Polyacrylic acid-coated iron oxide nanoparticles could be a useful tool for tracking inflammatory monocytes |
| title_fullStr | Polyacrylic acid-coated iron oxide nanoparticles could be a useful tool for tracking inflammatory monocytes |
| title_full_unstemmed | Polyacrylic acid-coated iron oxide nanoparticles could be a useful tool for tracking inflammatory monocytes |
| title_short | Polyacrylic acid-coated iron oxide nanoparticles could be a useful tool for tracking inflammatory monocytes |
| title_sort | polyacrylic acid coated iron oxide nanoparticles could be a useful tool for tracking inflammatory monocytes |
| topic | cytokines inflammation iron oxide nanoparticles monocyte-derived macrophages monocytes mononuclear phagocytes |
| url | https://www.future-science.com/doi/10.2144/fsoa-2019-0066 |
| work_keys_str_mv | AT manuelagiraldovillegas polyacrylicacidcoatedironoxidenanoparticlescouldbeausefultoolfortrackinginflammatorymonocytes AT jeanethurquijo polyacrylicacidcoatedironoxidenanoparticlescouldbeausefultoolfortrackinginflammatorymonocytes AT oscarlarnacheolmos polyacrylicacidcoatedironoxidenanoparticlescouldbeausefultoolfortrackinginflammatorymonocytes AT mauriciorojaslopez polyacrylicacidcoatedironoxidenanoparticlescouldbeausefultoolfortrackinginflammatorymonocytes |