Decreased expression of miR-200a and miR-223-3p in endometriosis during the secretory phase of menstrual cycle: Insights from a case-control study on molecular biomarkers and disease-related infertility
Background: Endometriosis (EM) is a condition that causes infertility with decreasing uterine receptivity. It is reported that it affects about 20–25% of all infertile women. Some genetic markers play a crucial role in pathogenesis and infertility. Objective: This study investigates the role of...
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Shahid Sadoughi University of Medical Sciences
2025-02-01
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| Series: | International Journal of Reproductive BioMedicine |
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| Online Access: | https://knepublishing.com/index.php/ijrm/article/view/18066 |
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| author | Yasaman Nazari Hagh Mohamadreza Ahmadifard Sedigheh Esmaelzadeh Soheila Abbaszadeh Naser Shokrzadeh |
| author_facet | Yasaman Nazari Hagh Mohamadreza Ahmadifard Sedigheh Esmaelzadeh Soheila Abbaszadeh Naser Shokrzadeh |
| author_sort | Yasaman Nazari Hagh |
| collection | DOAJ |
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Background: Endometriosis (EM) is a condition that causes infertility with decreasing uterine receptivity. It is reported that it affects about 20–25% of all infertile women. Some genetic markers play a crucial role in pathogenesis and infertility.
Objective: This study investigates the role of miR-200a and miR-223-3p in embryo implantation and their association with EM-related infertility.
Materials and Methods: In this case-control study, 36 women who referred to the Center for Research on Reproductive Health and Infertility of Babol University of Medical Sciences and Fatemeh Al-Zahra Infertility Specialized Treatment Center in Babol, Iran between June 2022 and July 2023 were evaluated. Participants were divided into 2 EM and control groups (n = 18/each). Endometrial samples were collected from participants between 17th and 24th days of their menstrual cycle. Histopathological examination (hematoxylin and eosin and periodic acid schiff) was performed to confirm the secretory stage, and miR-200a and miR-223-3p expressions were analyzed by quantitative reverse transcriptase-polymerase chain reaction.
Results: Histological analysis confirmed that both groups were in the secretory stage. Additionally, miRNA expression results showed a significant decrease in the miR-200a and miR-223-3p expression levels in EM group compared to control group. The expression level of miR-223-3p and miR-200a in the eutopic endometrial tissue of women with EM was notably lower than those in the control group.
Conclusion: Our results suggest that miR-200a and miR-223-3p are involved in the EM pathogenesis, while other genes and signaling pathways are probably involved in the implantation failure.
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| format | Article |
| id | doaj-art-ad45ded938a5482facb81e960bcee345 |
| institution | Kabale University |
| issn | 2476-4108 2476-3772 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj-art-ad45ded938a5482facb81e960bcee3452025-02-08T12:09:24ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722025-02-01221210.18502/ijrm.v22i12.18066Decreased expression of miR-200a and miR-223-3p in endometriosis during the secretory phase of menstrual cycle: Insights from a case-control study on molecular biomarkers and disease-related infertilityYasaman Nazari Hagh0Mohamadreza Ahmadifard1Sedigheh Esmaelzadeh2 Soheila Abbaszadeh3Naser Shokrzadeh4School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.Department of Medical Genetics, School of Medicine, Babol University of Medical Sciences, Babol, Iran. Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.Reproductive Health Research Center, Clinical Research Institute, Urmia University of Medical Sciences, Urmia, Iran Background: Endometriosis (EM) is a condition that causes infertility with decreasing uterine receptivity. It is reported that it affects about 20–25% of all infertile women. Some genetic markers play a crucial role in pathogenesis and infertility. Objective: This study investigates the role of miR-200a and miR-223-3p in embryo implantation and their association with EM-related infertility. Materials and Methods: In this case-control study, 36 women who referred to the Center for Research on Reproductive Health and Infertility of Babol University of Medical Sciences and Fatemeh Al-Zahra Infertility Specialized Treatment Center in Babol, Iran between June 2022 and July 2023 were evaluated. Participants were divided into 2 EM and control groups (n = 18/each). Endometrial samples were collected from participants between 17th and 24th days of their menstrual cycle. Histopathological examination (hematoxylin and eosin and periodic acid schiff) was performed to confirm the secretory stage, and miR-200a and miR-223-3p expressions were analyzed by quantitative reverse transcriptase-polymerase chain reaction. Results: Histological analysis confirmed that both groups were in the secretory stage. Additionally, miRNA expression results showed a significant decrease in the miR-200a and miR-223-3p expression levels in EM group compared to control group. The expression level of miR-223-3p and miR-200a in the eutopic endometrial tissue of women with EM was notably lower than those in the control group. Conclusion: Our results suggest that miR-200a and miR-223-3p are involved in the EM pathogenesis, while other genes and signaling pathways are probably involved in the implantation failure. https://knepublishing.com/index.php/ijrm/article/view/18066Endometriosis, miRNA, Implantation, Signaling pathways, Biomarkers, Infertility, Menstrual cycle. |
| spellingShingle | Yasaman Nazari Hagh Mohamadreza Ahmadifard Sedigheh Esmaelzadeh Soheila Abbaszadeh Naser Shokrzadeh Decreased expression of miR-200a and miR-223-3p in endometriosis during the secretory phase of menstrual cycle: Insights from a case-control study on molecular biomarkers and disease-related infertility International Journal of Reproductive BioMedicine Endometriosis, miRNA, Implantation, Signaling pathways, Biomarkers, Infertility, Menstrual cycle. |
| title | Decreased expression of miR-200a and miR-223-3p in endometriosis during the secretory phase of menstrual cycle: Insights from a case-control study on molecular biomarkers and disease-related infertility |
| title_full | Decreased expression of miR-200a and miR-223-3p in endometriosis during the secretory phase of menstrual cycle: Insights from a case-control study on molecular biomarkers and disease-related infertility |
| title_fullStr | Decreased expression of miR-200a and miR-223-3p in endometriosis during the secretory phase of menstrual cycle: Insights from a case-control study on molecular biomarkers and disease-related infertility |
| title_full_unstemmed | Decreased expression of miR-200a and miR-223-3p in endometriosis during the secretory phase of menstrual cycle: Insights from a case-control study on molecular biomarkers and disease-related infertility |
| title_short | Decreased expression of miR-200a and miR-223-3p in endometriosis during the secretory phase of menstrual cycle: Insights from a case-control study on molecular biomarkers and disease-related infertility |
| title_sort | decreased expression of mir 200a and mir 223 3p in endometriosis during the secretory phase of menstrual cycle insights from a case control study on molecular biomarkers and disease related infertility |
| topic | Endometriosis, miRNA, Implantation, Signaling pathways, Biomarkers, Infertility, Menstrual cycle. |
| url | https://knepublishing.com/index.php/ijrm/article/view/18066 |
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