Impact of non-thermal plasma seed priming and early development stages of two local Thai Cruciferous plants mustard green and rat-tailed radish on glucosinolates, isothiocyanates, minerals, antioxidant and anticancer activities
Over the past decade, non-thermal plasma (NTP) technology has emerged as a promising tool in the food sector. Cruciferous microgreens are known for their anticancer properties, yet the potential of certain varieties at early developmental stages remains underexplored. This study investigated the ef...
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AcademicPres
2025-02-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/14149 |
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| author | Thipphiya KARIRAT Worachot SAENGHA Khanit MATRA Kannika NAKHOWONG Pimsupa PATTANU Piyatida KITKAYUN Theerayut BUBPAMALA Benjaporn BURANRAT Sirirat DEESEENTHUM Teeraporn KATISART Vijitra LUANG-IN |
| author_facet | Thipphiya KARIRAT Worachot SAENGHA Khanit MATRA Kannika NAKHOWONG Pimsupa PATTANU Piyatida KITKAYUN Theerayut BUBPAMALA Benjaporn BURANRAT Sirirat DEESEENTHUM Teeraporn KATISART Vijitra LUANG-IN |
| author_sort | Thipphiya KARIRAT |
| collection | DOAJ |
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Over the past decade, non-thermal plasma (NTP) technology has emerged as a promising tool in the food sector. Cruciferous microgreens are known for their anticancer properties, yet the potential of certain varieties at early developmental stages remains underexplored. This study investigated the effects of NTP treatment on seed priming and plant development at 14, 21, and 28 days in mustard green (MG) (Brassica juncea (L.) Czern) and rat-tailed radish (RTR) (Raphanus sativus var. caudatus), focusing on their bioactive compounds and bioactivities. NTP treatment significantly affects stem length, fresh weight, or dry weight compared to untreated seeds. It also enhanced the production of glucosinolates, isothiocyanates (ITCs), specific minerals, total phenolics, total flavonoids, and biological activities in both plants. MG was found to contain sinigrin, gluconapin, allyl ITC, and 3-butenyl ITC, while RTR contained glucoraphasatin and raphasatin. MG exhibited IC50 values ranging from 16–78 µg/mL in cytotoxicity tests against four cancer cell lines HeLa, HepG2, MCF-7 and HT-29 with enhanced activity from NTP. RTR demonstrated greater effectiveness with IC50 values of 12–60 µg/mL with higher activity from NTP. Both plant extracts especially NTP-treated samples reduced cancer cell survival and proliferation by upregulating pro-apoptotic genes (Bax, caspase-3, and p21) and proteins while downregulating anti-apoptotic and metastatic markers (Bcl-2, MMP-9, MMP-2, and cyclin D1). NTP can enhance the therapeutic bioactivity of young plants, with both MG and RTR at 14 and 21 days of growth, showing the higher potential for anticancer applications.
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| format | Article |
| id | doaj-art-ad3c60dbdd1e4b1e91bbcb80d7f3e088 |
| institution | DOAJ |
| issn | 0255-965X 1842-4309 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | AcademicPres |
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| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj-art-ad3c60dbdd1e4b1e91bbcb80d7f3e0882025-08-20T03:03:45ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092025-02-0153110.15835/nbha53114149Impact of non-thermal plasma seed priming and early development stages of two local Thai Cruciferous plants mustard green and rat-tailed radish on glucosinolates, isothiocyanates, minerals, antioxidant and anticancer activitiesThipphiya KARIRAT0Worachot SAENGHA1Khanit MATRA2Kannika NAKHOWONG3Pimsupa PATTANU4Piyatida KITKAYUN5Theerayut BUBPAMALA6Benjaporn BURANRAT7Sirirat DEESEENTHUM8Teeraporn KATISART9Vijitra LUANG-IN10Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Maha Sarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Maha Sarakham 44150Srinakharinwirot University, Faculty of Engineering, Department of Electrical Engineering, Nakhon Nayok 26120Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Maha Sarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Maha Sarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Maha Sarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Maha Sarakham 44150Mahasarakham University, Faculty of Medicine, Mahasarakham 44000Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Maha Sarakham 44150Mahasarakham University, Faculty of Science, Department of Biology, Maha Sarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Maha Sarakham 44150 Over the past decade, non-thermal plasma (NTP) technology has emerged as a promising tool in the food sector. Cruciferous microgreens are known for their anticancer properties, yet the potential of certain varieties at early developmental stages remains underexplored. This study investigated the effects of NTP treatment on seed priming and plant development at 14, 21, and 28 days in mustard green (MG) (Brassica juncea (L.) Czern) and rat-tailed radish (RTR) (Raphanus sativus var. caudatus), focusing on their bioactive compounds and bioactivities. NTP treatment significantly affects stem length, fresh weight, or dry weight compared to untreated seeds. It also enhanced the production of glucosinolates, isothiocyanates (ITCs), specific minerals, total phenolics, total flavonoids, and biological activities in both plants. MG was found to contain sinigrin, gluconapin, allyl ITC, and 3-butenyl ITC, while RTR contained glucoraphasatin and raphasatin. MG exhibited IC50 values ranging from 16–78 µg/mL in cytotoxicity tests against four cancer cell lines HeLa, HepG2, MCF-7 and HT-29 with enhanced activity from NTP. RTR demonstrated greater effectiveness with IC50 values of 12–60 µg/mL with higher activity from NTP. Both plant extracts especially NTP-treated samples reduced cancer cell survival and proliferation by upregulating pro-apoptotic genes (Bax, caspase-3, and p21) and proteins while downregulating anti-apoptotic and metastatic markers (Bcl-2, MMP-9, MMP-2, and cyclin D1). NTP can enhance the therapeutic bioactivity of young plants, with both MG and RTR at 14 and 21 days of growth, showing the higher potential for anticancer applications. https://www.notulaebotanicae.ro/index.php/nbha/article/view/14149apoptosisallyl isothiocyanatesBrassica juncea3-butenyl isothiocyanatesRaphanus sativusraphasatin |
| spellingShingle | Thipphiya KARIRAT Worachot SAENGHA Khanit MATRA Kannika NAKHOWONG Pimsupa PATTANU Piyatida KITKAYUN Theerayut BUBPAMALA Benjaporn BURANRAT Sirirat DEESEENTHUM Teeraporn KATISART Vijitra LUANG-IN Impact of non-thermal plasma seed priming and early development stages of two local Thai Cruciferous plants mustard green and rat-tailed radish on glucosinolates, isothiocyanates, minerals, antioxidant and anticancer activities Notulae Botanicae Horti Agrobotanici Cluj-Napoca apoptosis allyl isothiocyanates Brassica juncea 3-butenyl isothiocyanates Raphanus sativus raphasatin |
| title | Impact of non-thermal plasma seed priming and early development stages of two local Thai Cruciferous plants mustard green and rat-tailed radish on glucosinolates, isothiocyanates, minerals, antioxidant and anticancer activities |
| title_full | Impact of non-thermal plasma seed priming and early development stages of two local Thai Cruciferous plants mustard green and rat-tailed radish on glucosinolates, isothiocyanates, minerals, antioxidant and anticancer activities |
| title_fullStr | Impact of non-thermal plasma seed priming and early development stages of two local Thai Cruciferous plants mustard green and rat-tailed radish on glucosinolates, isothiocyanates, minerals, antioxidant and anticancer activities |
| title_full_unstemmed | Impact of non-thermal plasma seed priming and early development stages of two local Thai Cruciferous plants mustard green and rat-tailed radish on glucosinolates, isothiocyanates, minerals, antioxidant and anticancer activities |
| title_short | Impact of non-thermal plasma seed priming and early development stages of two local Thai Cruciferous plants mustard green and rat-tailed radish on glucosinolates, isothiocyanates, minerals, antioxidant and anticancer activities |
| title_sort | impact of non thermal plasma seed priming and early development stages of two local thai cruciferous plants mustard green and rat tailed radish on glucosinolates isothiocyanates minerals antioxidant and anticancer activities |
| topic | apoptosis allyl isothiocyanates Brassica juncea 3-butenyl isothiocyanates Raphanus sativus raphasatin |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/14149 |
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