Enhancing fungal adaptation for efficient caffeine degradation in wastewater: Biomimetic approach and environmental optimization
This study introduces an innovative approach to enhance fungal adaptation and degradation capabilities for treating caffeine, a prevalent environmental pollutant, within wastewater systems. Pure caffeine, extracted from coffee, was thoroughly characterized using Fourier Transform Infrared Spectrosco...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Desalination and Water Treatment |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1944398624204483 |
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| author | Bhautik Dave Ewa Lobos Moysa Anna Kuźnik |
| author_facet | Bhautik Dave Ewa Lobos Moysa Anna Kuźnik |
| author_sort | Bhautik Dave |
| collection | DOAJ |
| description | This study introduces an innovative approach to enhance fungal adaptation and degradation capabilities for treating caffeine, a prevalent environmental pollutant, within wastewater systems. Pure caffeine, extracted from coffee, was thoroughly characterized using Fourier Transform Infrared Spectroscopy (FT-IR) and Nuclear Magnetic Resonance (NMR). The fungal species Trametes versicolor was selected, cultured, and employed in experiments targeting caffeine degradation in potato dextrose broth (PDB) and synthetic wastewater (SWW) media. The caffeine concentration was standardized at 1 mg/10 ml across conditions, with experiments conducted at two temperatures (25 °C and 37 °C) and pH levels (2.5 and 5.18). Additionally, degradation tests were conducted on 100 ml of coffee extract to simulate real-world conditions. Results demonstrated up to 98 % degradation efficiencies under optimized parameters, although higher temperatures and more acidic pH conditions reduced efficacy. Despite some residual compounds observed in NMR spectra, caffeine levels were significantly reduced, achieving 90 % and 98 % degradation rates. These findings underscore the importance of temperature and pH optimization for enhancing caffeine degradation. The methodology leveraged a direct cultivation and adaptation approach for T. versicolor, initiating fungal growth directly from spores within aqueous environments, thereby facilitating its integration into wastewater treatment systems. |
| format | Article |
| id | doaj-art-ad3bc3205afb4e4db2ae894dd08181f3 |
| institution | DOAJ |
| issn | 1944-3986 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Desalination and Water Treatment |
| spelling | doaj-art-ad3bc3205afb4e4db2ae894dd08181f32025-08-20T03:04:46ZengElsevierDesalination and Water Treatment1944-39862025-01-0132110093810.1016/j.dwt.2024.100938Enhancing fungal adaptation for efficient caffeine degradation in wastewater: Biomimetic approach and environmental optimizationBhautik Dave0Ewa Lobos Moysa1Anna Kuźnik2Department of Water and Wastewater Engineering, Silesian University of Technology, Gliwice, Poland; Corresponding author.Department of Water and Wastewater Engineering, Silesian University of Technology, Gliwice, PolandDepartment of Organic Chemistry, Bioorganic Chemistry and Biotechnology, Silesian University of Technology, B. Krzywoustego 4, 44-100 Gliwice, Poland; Biotechnology Center of Silesian University of Technology, B. Krzywoustego 8, 44-100 Gliwice, PolandThis study introduces an innovative approach to enhance fungal adaptation and degradation capabilities for treating caffeine, a prevalent environmental pollutant, within wastewater systems. Pure caffeine, extracted from coffee, was thoroughly characterized using Fourier Transform Infrared Spectroscopy (FT-IR) and Nuclear Magnetic Resonance (NMR). The fungal species Trametes versicolor was selected, cultured, and employed in experiments targeting caffeine degradation in potato dextrose broth (PDB) and synthetic wastewater (SWW) media. The caffeine concentration was standardized at 1 mg/10 ml across conditions, with experiments conducted at two temperatures (25 °C and 37 °C) and pH levels (2.5 and 5.18). Additionally, degradation tests were conducted on 100 ml of coffee extract to simulate real-world conditions. Results demonstrated up to 98 % degradation efficiencies under optimized parameters, although higher temperatures and more acidic pH conditions reduced efficacy. Despite some residual compounds observed in NMR spectra, caffeine levels were significantly reduced, achieving 90 % and 98 % degradation rates. These findings underscore the importance of temperature and pH optimization for enhancing caffeine degradation. The methodology leveraged a direct cultivation and adaptation approach for T. versicolor, initiating fungal growth directly from spores within aqueous environments, thereby facilitating its integration into wastewater treatment systems.http://www.sciencedirect.com/science/article/pii/S1944398624204483Fungal adaptationCaffeine degradationWastewater treatmentBiomimetic approachDegradation efficiencyMicrobial Remediation |
| spellingShingle | Bhautik Dave Ewa Lobos Moysa Anna Kuźnik Enhancing fungal adaptation for efficient caffeine degradation in wastewater: Biomimetic approach and environmental optimization Desalination and Water Treatment Fungal adaptation Caffeine degradation Wastewater treatment Biomimetic approach Degradation efficiency Microbial Remediation |
| title | Enhancing fungal adaptation for efficient caffeine degradation in wastewater: Biomimetic approach and environmental optimization |
| title_full | Enhancing fungal adaptation for efficient caffeine degradation in wastewater: Biomimetic approach and environmental optimization |
| title_fullStr | Enhancing fungal adaptation for efficient caffeine degradation in wastewater: Biomimetic approach and environmental optimization |
| title_full_unstemmed | Enhancing fungal adaptation for efficient caffeine degradation in wastewater: Biomimetic approach and environmental optimization |
| title_short | Enhancing fungal adaptation for efficient caffeine degradation in wastewater: Biomimetic approach and environmental optimization |
| title_sort | enhancing fungal adaptation for efficient caffeine degradation in wastewater biomimetic approach and environmental optimization |
| topic | Fungal adaptation Caffeine degradation Wastewater treatment Biomimetic approach Degradation efficiency Microbial Remediation |
| url | http://www.sciencedirect.com/science/article/pii/S1944398624204483 |
| work_keys_str_mv | AT bhautikdave enhancingfungaladaptationforefficientcaffeinedegradationinwastewaterbiomimeticapproachandenvironmentaloptimization AT ewalobosmoysa enhancingfungaladaptationforefficientcaffeinedegradationinwastewaterbiomimeticapproachandenvironmentaloptimization AT annakuznik enhancingfungaladaptationforefficientcaffeinedegradationinwastewaterbiomimeticapproachandenvironmentaloptimization |