Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case
Light and transmission electron microscopy observations are reported on the structure and in vitro cytopathic effect of Acanthamoeba griffini trophozoites isolated from a clinical case. Live trophozoites were moderately active with a remarkable pleomorphism which changed from ovoid to quite elongate...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/256310 |
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| author | Arturo González-Robles Lizbeth Salazar-Villatoro Maritza Omaña-Molina Maria Reyes-Batlle Carmen M. Martín-Navarro Jacob Lorenzo-Morales |
| author_facet | Arturo González-Robles Lizbeth Salazar-Villatoro Maritza Omaña-Molina Maria Reyes-Batlle Carmen M. Martín-Navarro Jacob Lorenzo-Morales |
| author_sort | Arturo González-Robles |
| collection | DOAJ |
| description | Light and transmission electron microscopy observations are reported on the structure and in vitro cytopathic effect of Acanthamoeba griffini trophozoites isolated from a clinical case. Live trophozoites were moderately active with a remarkable pleomorphism which changed from ovoid to quite elongated shapes. When moving, amoebae formed cytoplasmic projections such as wide lamellae and acanthopodia of diverse size and thickness which contain a significant amount of actin. Ultrastructurally, the cytoplasm showed the main organelles found in other free-living amoebae. Coincubation of trophozoites with MDCK cell monolayers resulted in a local damage to target cells after 24 h of interaction, suggesting that the cytopathic effect is contact-dependent. By transmission electron microscopy, amoebae appeared to engulf small portions of the MDCK cells; however, the cells that were not in contact with trophozoites had an unaltered morphology. When epithelial monolayers were incubated with conditioned medium for 24 h, small areas of cell injury were also observed. The phylogenetical analysis as well as the sequencing of the acquired amplified product for the DF3 region of the amoebae isolate confirmed that it belongs to genotype T3, which includes other pathogenic amoebae; besides the activity of two drugs currently used against Acanthamoeba was tested on A. griffini. |
| format | Article |
| id | doaj-art-ad334cc294324f05ac043017c387aa62 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | Journal of Parasitology Research |
| spelling | doaj-art-ad334cc294324f05ac043017c387aa622025-02-03T07:26:17ZengWileyJournal of Parasitology Research2090-00232090-00312014-01-01201410.1155/2014/256310256310Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical CaseArturo González-Robles0Lizbeth Salazar-Villatoro1Maritza Omaña-Molina2Maria Reyes-Batlle3Carmen M. Martín-Navarro4Jacob Lorenzo-Morales5Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, CINVESTAV-IPN, Avenida Instituto Politécnico Nacional 2508, San Pedro Zacatenco, 07360 Mexico, DF, MexicoDepartment of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, CINVESTAV-IPN, Avenida Instituto Politécnico Nacional 2508, San Pedro Zacatenco, 07360 Mexico, DF, MexicoFaculty of Superior Studies, Biology, UNAM, Los Reyes Iztacala, 54090 Tlalnepantla, MEX, MexicoUniversity Institute of Tropical Diseases and Public Health of The Canary Islands, University of La Laguna, Avenida Astrofísico Francisco Sánchez SN, San Cristóbal de La Laguna, Tenerife, 38203 Canary Islands, SpainUniversity Institute of Tropical Diseases and Public Health of The Canary Islands, University of La Laguna, Avenida Astrofísico Francisco Sánchez SN, San Cristóbal de La Laguna, Tenerife, 38203 Canary Islands, SpainUniversity Institute of Tropical Diseases and Public Health of The Canary Islands, University of La Laguna, Avenida Astrofísico Francisco Sánchez SN, San Cristóbal de La Laguna, Tenerife, 38203 Canary Islands, SpainLight and transmission electron microscopy observations are reported on the structure and in vitro cytopathic effect of Acanthamoeba griffini trophozoites isolated from a clinical case. Live trophozoites were moderately active with a remarkable pleomorphism which changed from ovoid to quite elongated shapes. When moving, amoebae formed cytoplasmic projections such as wide lamellae and acanthopodia of diverse size and thickness which contain a significant amount of actin. Ultrastructurally, the cytoplasm showed the main organelles found in other free-living amoebae. Coincubation of trophozoites with MDCK cell monolayers resulted in a local damage to target cells after 24 h of interaction, suggesting that the cytopathic effect is contact-dependent. By transmission electron microscopy, amoebae appeared to engulf small portions of the MDCK cells; however, the cells that were not in contact with trophozoites had an unaltered morphology. When epithelial monolayers were incubated with conditioned medium for 24 h, small areas of cell injury were also observed. The phylogenetical analysis as well as the sequencing of the acquired amplified product for the DF3 region of the amoebae isolate confirmed that it belongs to genotype T3, which includes other pathogenic amoebae; besides the activity of two drugs currently used against Acanthamoeba was tested on A. griffini.http://dx.doi.org/10.1155/2014/256310 |
| spellingShingle | Arturo González-Robles Lizbeth Salazar-Villatoro Maritza Omaña-Molina Maria Reyes-Batlle Carmen M. Martín-Navarro Jacob Lorenzo-Morales Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case Journal of Parasitology Research |
| title | Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case |
| title_full | Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case |
| title_fullStr | Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case |
| title_full_unstemmed | Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case |
| title_short | Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case |
| title_sort | morphological features and in vitro cytopathic effect of acanthamoeba griffini trophozoites isolated from a clinical case |
| url | http://dx.doi.org/10.1155/2014/256310 |
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