The Effect of Lyophilized and Frozen Umbilical Cord Cryoextract on L929 Cell Culture
Background. The human umbilical cord is a promising source of biologically active substances with regenerative properties. However, the potential of lyophilized cryoextract from the umbilical cord for regenerative medicine, which could facilitate storage and transportation, remains unexplored. Ther...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Igor Sikorsky Kyiv Polytechnic Institute
2025-01-01
|
| Series: | Innovative Biosystems and Bioengineering |
| Online Access: | https://ibb.kpi.ua/article/view/313718 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849769380227842048 |
|---|---|
| author | Anna Kaverinska Nadiia Shevchenko Aleksandr Osetsky Liudmyla Sukhodub Viktoriya Lazurenko Oleksandr Zhelezniakov Volodymyr Prokopiuk |
| author_facet | Anna Kaverinska Nadiia Shevchenko Aleksandr Osetsky Liudmyla Sukhodub Viktoriya Lazurenko Oleksandr Zhelezniakov Volodymyr Prokopiuk |
| author_sort | Anna Kaverinska |
| collection | DOAJ |
| description |
Background. The human umbilical cord is a promising source of biologically active substances with regenerative properties. However, the potential of lyophilized cryoextract from the umbilical cord for regenerative medicine, which could facilitate storage and transportation, remains unexplored. Therefore, it is important to study the effect of such cryoextracts using a cellular model.
Objective. To evaluate the effect of lyophilized and frozen umbilical cord cryoextracts on the L929 cell line to assess their therapeutic potential.
Methods. This study was conducted on L929 cell cultures. Cryoextracts from the human umbilical cord were obtained through cryoextraction and lyophilized forms at -80 and -20 °C. These extracts were added to Dulbecco's Modified Eagle Medium (DMEM) at three concentrations: 0.1, 0.5, and 1.0 mg/ml. The control groups included cells cultured in DMEM with and without fetal bovine serum. Cell morphology and monolayer confluency were observed. To assess the impact of the cryoextracts, several assays were performed: cell viability (adhesion), migration activity (scratch test), pinocytosis activity (neutral red uptake assay), metabolic activity (MTT assay), and (proliferation) population doubling time.
Results. The addition of umbilical cord cryoextract and its lyophilized form at -80 °C was non-toxic to the cells. The most effective concentration was 0.1 mg/ml, which significantly stimulated cell adhesion and proliferation compared to the culture medium without fetal serum. The lyophilized cryoextract at -20 °C did not enhance cell viability but did increase pinocytosis activity.
Conclusions. These findings suggest that umbilical cord cryoextract and its lyophilized form at -80 °C can be used as growth factors in cell line cultivation. The lyophilized cryoextract shows promise for use in conditions where specialized storage equipment is not available. However, the lyophilized form at -20 °C primarily stimulates pinocytosis activity and inhibits proliferation.
|
| format | Article |
| id | doaj-art-ad2fdb6407d74a34931fcac2c700d7ad |
| institution | DOAJ |
| issn | 2616-177X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Igor Sikorsky Kyiv Polytechnic Institute |
| record_format | Article |
| series | Innovative Biosystems and Bioengineering |
| spelling | doaj-art-ad2fdb6407d74a34931fcac2c700d7ad2025-08-20T03:03:25ZengIgor Sikorsky Kyiv Polytechnic InstituteInnovative Biosystems and Bioengineering2616-177X2025-01-019110.20535/ibb.2025.9.1.313718The Effect of Lyophilized and Frozen Umbilical Cord Cryoextract on L929 Cell CultureAnna Kaverinska0Nadiia Shevchenko1Aleksandr Osetsky2Liudmyla Sukhodub3Viktoriya Lazurenko4Oleksandr Zhelezniakov5Volodymyr Prokopiuk6Institute for Problems of Cryobiology and Cryomedicine of NAS of UkraineInstitute for Problems of Cryobiology and Cryomedicine of NAS of UkraineInstitute for Problems of Cryobiology and Cryomedicine of NAS of UkraineSumy State UniversityKharkiv National Medical UniversityKharkiv National Medical UniversityInstitute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine; Kharkiv National Medical University Background. The human umbilical cord is a promising source of biologically active substances with regenerative properties. However, the potential of lyophilized cryoextract from the umbilical cord for regenerative medicine, which could facilitate storage and transportation, remains unexplored. Therefore, it is important to study the effect of such cryoextracts using a cellular model. Objective. To evaluate the effect of lyophilized and frozen umbilical cord cryoextracts on the L929 cell line to assess their therapeutic potential. Methods. This study was conducted on L929 cell cultures. Cryoextracts from the human umbilical cord were obtained through cryoextraction and lyophilized forms at -80 and -20 °C. These extracts were added to Dulbecco's Modified Eagle Medium (DMEM) at three concentrations: 0.1, 0.5, and 1.0 mg/ml. The control groups included cells cultured in DMEM with and without fetal bovine serum. Cell morphology and monolayer confluency were observed. To assess the impact of the cryoextracts, several assays were performed: cell viability (adhesion), migration activity (scratch test), pinocytosis activity (neutral red uptake assay), metabolic activity (MTT assay), and (proliferation) population doubling time. Results. The addition of umbilical cord cryoextract and its lyophilized form at -80 °C was non-toxic to the cells. The most effective concentration was 0.1 mg/ml, which significantly stimulated cell adhesion and proliferation compared to the culture medium without fetal serum. The lyophilized cryoextract at -20 °C did not enhance cell viability but did increase pinocytosis activity. Conclusions. These findings suggest that umbilical cord cryoextract and its lyophilized form at -80 °C can be used as growth factors in cell line cultivation. The lyophilized cryoextract shows promise for use in conditions where specialized storage equipment is not available. However, the lyophilized form at -20 °C primarily stimulates pinocytosis activity and inhibits proliferation. https://ibb.kpi.ua/article/view/313718 |
| spellingShingle | Anna Kaverinska Nadiia Shevchenko Aleksandr Osetsky Liudmyla Sukhodub Viktoriya Lazurenko Oleksandr Zhelezniakov Volodymyr Prokopiuk The Effect of Lyophilized and Frozen Umbilical Cord Cryoextract on L929 Cell Culture Innovative Biosystems and Bioengineering |
| title | The Effect of Lyophilized and Frozen Umbilical Cord Cryoextract on L929 Cell Culture |
| title_full | The Effect of Lyophilized and Frozen Umbilical Cord Cryoextract on L929 Cell Culture |
| title_fullStr | The Effect of Lyophilized and Frozen Umbilical Cord Cryoextract on L929 Cell Culture |
| title_full_unstemmed | The Effect of Lyophilized and Frozen Umbilical Cord Cryoextract on L929 Cell Culture |
| title_short | The Effect of Lyophilized and Frozen Umbilical Cord Cryoextract on L929 Cell Culture |
| title_sort | effect of lyophilized and frozen umbilical cord cryoextract on l929 cell culture |
| url | https://ibb.kpi.ua/article/view/313718 |
| work_keys_str_mv | AT annakaverinska theeffectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT nadiiashevchenko theeffectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT aleksandrosetsky theeffectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT liudmylasukhodub theeffectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT viktoriyalazurenko theeffectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT oleksandrzhelezniakov theeffectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT volodymyrprokopiuk theeffectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT annakaverinska effectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT nadiiashevchenko effectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT aleksandrosetsky effectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT liudmylasukhodub effectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT viktoriyalazurenko effectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT oleksandrzhelezniakov effectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture AT volodymyrprokopiuk effectoflyophilizedandfrozenumbilicalcordcryoextractonl929cellculture |