Chemokine Receptor Expression on Normal Blood CD56+ NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population
Studies of chemokine receptors (CKR) in natural killer- (NK-) cells have already been published, but only a few gave detailed information on its differential expression on blood NK-cell subsets. We report on the expression of the inflammatory and homeostatic CKR on normal blood CD56+low CD16+ and CD...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/839684 |
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| author | Margarida Lima Magdalena Leander Marlene Santos Ana Helena Santos Catarina Lau Maria Luís Queirós Marta Gonçalves Sónia Fonseca João Moura Maria dos Anjos Teixeira Alberto Orfao |
| author_facet | Margarida Lima Magdalena Leander Marlene Santos Ana Helena Santos Catarina Lau Maria Luís Queirós Marta Gonçalves Sónia Fonseca João Moura Maria dos Anjos Teixeira Alberto Orfao |
| author_sort | Margarida Lima |
| collection | DOAJ |
| description | Studies of chemokine receptors (CKR) in natural killer- (NK-) cells have already been published, but only a few gave detailed information on its differential expression on blood NK-cell subsets. We report on the expression of the inflammatory and homeostatic CKR on normal blood CD56+low CD16+ and CD56+high CD16-/+low NK-cells. Conventional CD56+low and CD56+high NK-cells present in the normal PB do express CKR for inflammatory cytokines, although with different patterns CD56+low NK-cells are mainly CXCR1/CXCR2+ and CXCR3/CCR5−/+, whereas mostly CD56+high NK-cells are CXCR1/CXCR2− and CXCR3/CCR5+. Both NK-cell subsets have variable CXCR4 expression and are CCR4− and CCR6−. The CKR repertoire of the CD56+low NK-cells approaches to that of neutrophils, whereas the CKR repertoire of the CD56+high NK-cells mimics that of Th1+ T cells, suggesting that these cells are prepared to migrate into inflamed tissues at different phases of the immune response. In addition, we describe a subpopulation of NK-cells with intermediate levels of CD56 expression, which we named CD56+int NK-cells. These NK-cells are CXCR3/CCR5+, they have intermediate levels of expression of CD16, CD62L, CD94, and CD122, and they are CD57− and CD158a−. In view of their phenotypic features, we hypothesize that they correspond to a transitional stage, between the well-known CD56+high and CD56+low NK-cells populations. |
| format | Article |
| id | doaj-art-ad2e4374f6924752ac7fd5faffc67649 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-ad2e4374f6924752ac7fd5faffc676492025-08-20T03:38:55ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/839684839684Chemokine Receptor Expression on Normal Blood CD56+ NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell PopulationMargarida Lima0Magdalena Leander1Marlene Santos2Ana Helena Santos3Catarina Lau4Maria Luís Queirós5Marta Gonçalves6Sónia Fonseca7João Moura8Maria dos Anjos Teixeira9Alberto Orfao10Laboratory of Cytometry, Service of Hematology, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, PortugalLaboratory of Cytometry, Service of Hematology, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, PortugalLaboratory of Cytometry, Service of Hematology, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, PortugalLaboratory of Cytometry, Service of Hematology, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, PortugalLaboratory of Cytometry, Service of Hematology, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, PortugalLaboratory of Cytometry, Service of Hematology, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, PortugalLaboratory of Cytometry, Service of Hematology, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, PortugalLaboratory of Cytometry, Service of Hematology, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, PortugalLaboratory of Cytometry, Service of Hematology, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, PortugalLaboratory of Cytometry, Service of Hematology, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, PortugalLaboratory of Flow Cytometry, Centro de Investigación del Cancer (CIC), Campus Miguel de Unamuno, 37007 Salamanca, SpainStudies of chemokine receptors (CKR) in natural killer- (NK-) cells have already been published, but only a few gave detailed information on its differential expression on blood NK-cell subsets. We report on the expression of the inflammatory and homeostatic CKR on normal blood CD56+low CD16+ and CD56+high CD16-/+low NK-cells. Conventional CD56+low and CD56+high NK-cells present in the normal PB do express CKR for inflammatory cytokines, although with different patterns CD56+low NK-cells are mainly CXCR1/CXCR2+ and CXCR3/CCR5−/+, whereas mostly CD56+high NK-cells are CXCR1/CXCR2− and CXCR3/CCR5+. Both NK-cell subsets have variable CXCR4 expression and are CCR4− and CCR6−. The CKR repertoire of the CD56+low NK-cells approaches to that of neutrophils, whereas the CKR repertoire of the CD56+high NK-cells mimics that of Th1+ T cells, suggesting that these cells are prepared to migrate into inflamed tissues at different phases of the immune response. In addition, we describe a subpopulation of NK-cells with intermediate levels of CD56 expression, which we named CD56+int NK-cells. These NK-cells are CXCR3/CCR5+, they have intermediate levels of expression of CD16, CD62L, CD94, and CD122, and they are CD57− and CD158a−. In view of their phenotypic features, we hypothesize that they correspond to a transitional stage, between the well-known CD56+high and CD56+low NK-cells populations.http://dx.doi.org/10.1155/2015/839684 |
| spellingShingle | Margarida Lima Magdalena Leander Marlene Santos Ana Helena Santos Catarina Lau Maria Luís Queirós Marta Gonçalves Sónia Fonseca João Moura Maria dos Anjos Teixeira Alberto Orfao Chemokine Receptor Expression on Normal Blood CD56+ NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population Journal of Immunology Research |
| title | Chemokine Receptor Expression on Normal Blood CD56+ NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population |
| title_full | Chemokine Receptor Expression on Normal Blood CD56+ NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population |
| title_fullStr | Chemokine Receptor Expression on Normal Blood CD56+ NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population |
| title_full_unstemmed | Chemokine Receptor Expression on Normal Blood CD56+ NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population |
| title_short | Chemokine Receptor Expression on Normal Blood CD56+ NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population |
| title_sort | chemokine receptor expression on normal blood cd56 nk cells elucidates cell partners that comigrate during the innate and adaptive immune responses and identifies a transitional nk cell population |
| url | http://dx.doi.org/10.1155/2015/839684 |
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