Interpenetrating network hydrogel-loaded embryonic stem cell-derived endocardial cells improves cardiac function after myocardial infarction
Abstract Background With an in-depth understanding of cardiac cell differentiation, cell therapy derived from stem cells has shown promising therapeutic effects in the treatment of myocardial infarction (MI). Although many types of cardiac or noncardiac cells have been found to play protective roles...
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BMC
2025-05-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | https://doi.org/10.1186/s12967-025-06603-2 |
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| author | Boshi Liu Laiping Zhang Xiao Guan Jie Liu Weinian Shou Xin Chen Xiaohui Li Dayan Cao |
| author_facet | Boshi Liu Laiping Zhang Xiao Guan Jie Liu Weinian Shou Xin Chen Xiaohui Li Dayan Cao |
| author_sort | Boshi Liu |
| collection | DOAJ |
| description | Abstract Background With an in-depth understanding of cardiac cell differentiation, cell therapy derived from stem cells has shown promising therapeutic effects in the treatment of myocardial infarction (MI). Although many types of cardiac or noncardiac cells have been found to play protective roles in MI, the specific role of endocardial cells (ECCs) in MI has not been reported. Methods The current study was designed to determine whether human embryonic stem cell (hESC)-derived endocardial cells (hESC-ECCs) could be protective against MI. We first developed a cell delivery system by constructing a photosensitive interpenetrating network hydrogel consisting of gelatin methacryloyl (GelMA) and silk fibroin methacryloyl (SilMA). The sorted hESC-ECCs were loaded into the delivery system and then injected into the pericardium cavity of the MI rats. Results These results show that the cell delivery system has good biocompatibility. Moreover, the delivered endocardial cells improved cardiac function and delayed capillary atrophy after MI. Further mechanistic analysis revealed that hESC-ECCs protect the mitochondria of cardiomyocytes from damage under oxidative stress and potentially promote the angiogenesis of cardiac endothelial cells. Conclusion Our results demonstrated that hESC-ECCs have the potential to serve as a cell therapy strategy for MI treatment by maintaining cardiomyocyte survival and facilitating angiogenesis. |
| format | Article |
| id | doaj-art-ad1c8edaf58c414c85744a9d018fe059 |
| institution | DOAJ |
| issn | 1479-5876 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj-art-ad1c8edaf58c414c85744a9d018fe0592025-08-20T03:22:08ZengBMCJournal of Translational Medicine1479-58762025-05-0123111710.1186/s12967-025-06603-2Interpenetrating network hydrogel-loaded embryonic stem cell-derived endocardial cells improves cardiac function after myocardial infarctionBoshi Liu0Laiping Zhang1Xiao Guan2Jie Liu3Weinian Shou4Xin Chen5Xiaohui Li6Dayan Cao7Institute of Materia Medica, Department of Pharmaceutics, College of Pharmacy, Third Military Medical University (Army Medical University)Institute of Materia Medica, Department of Pharmaceutics, College of Pharmacy, Third Military Medical University (Army Medical University)Chongqing Engineering Research Center of Pharmaceutical Sciences, Chongqing Medical and Pharmaceutical CollegeInstitute of Materia Medica, Department of Pharmaceutics, College of Pharmacy, Third Military Medical University (Army Medical University)Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of MedicineInstitute of Materia Medica, Department of Pharmaceutics, College of Pharmacy, Third Military Medical University (Army Medical University)Institute of Materia Medica, Department of Pharmaceutics, College of Pharmacy, Third Military Medical University (Army Medical University)Institute of Materia Medica, Department of Pharmaceutics, College of Pharmacy, Third Military Medical University (Army Medical University)Abstract Background With an in-depth understanding of cardiac cell differentiation, cell therapy derived from stem cells has shown promising therapeutic effects in the treatment of myocardial infarction (MI). Although many types of cardiac or noncardiac cells have been found to play protective roles in MI, the specific role of endocardial cells (ECCs) in MI has not been reported. Methods The current study was designed to determine whether human embryonic stem cell (hESC)-derived endocardial cells (hESC-ECCs) could be protective against MI. We first developed a cell delivery system by constructing a photosensitive interpenetrating network hydrogel consisting of gelatin methacryloyl (GelMA) and silk fibroin methacryloyl (SilMA). The sorted hESC-ECCs were loaded into the delivery system and then injected into the pericardium cavity of the MI rats. Results These results show that the cell delivery system has good biocompatibility. Moreover, the delivered endocardial cells improved cardiac function and delayed capillary atrophy after MI. Further mechanistic analysis revealed that hESC-ECCs protect the mitochondria of cardiomyocytes from damage under oxidative stress and potentially promote the angiogenesis of cardiac endothelial cells. Conclusion Our results demonstrated that hESC-ECCs have the potential to serve as a cell therapy strategy for MI treatment by maintaining cardiomyocyte survival and facilitating angiogenesis.https://doi.org/10.1186/s12967-025-06603-2Cell delivery systemEmbryonic stem cellsEndocardial cellsMyocardial infarction |
| spellingShingle | Boshi Liu Laiping Zhang Xiao Guan Jie Liu Weinian Shou Xin Chen Xiaohui Li Dayan Cao Interpenetrating network hydrogel-loaded embryonic stem cell-derived endocardial cells improves cardiac function after myocardial infarction Journal of Translational Medicine Cell delivery system Embryonic stem cells Endocardial cells Myocardial infarction |
| title | Interpenetrating network hydrogel-loaded embryonic stem cell-derived endocardial cells improves cardiac function after myocardial infarction |
| title_full | Interpenetrating network hydrogel-loaded embryonic stem cell-derived endocardial cells improves cardiac function after myocardial infarction |
| title_fullStr | Interpenetrating network hydrogel-loaded embryonic stem cell-derived endocardial cells improves cardiac function after myocardial infarction |
| title_full_unstemmed | Interpenetrating network hydrogel-loaded embryonic stem cell-derived endocardial cells improves cardiac function after myocardial infarction |
| title_short | Interpenetrating network hydrogel-loaded embryonic stem cell-derived endocardial cells improves cardiac function after myocardial infarction |
| title_sort | interpenetrating network hydrogel loaded embryonic stem cell derived endocardial cells improves cardiac function after myocardial infarction |
| topic | Cell delivery system Embryonic stem cells Endocardial cells Myocardial infarction |
| url | https://doi.org/10.1186/s12967-025-06603-2 |
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