Identification, isolation and pathogenicity of Aeromonas salmonicida and his-topathology of infected Oncorhynchus mykiss in Punjab and northern areas of Pakistan
Aeromonas salmonicida causes furunculosis in the Oncorhynchus mykiss (rainbow trout) and the effective control over this infection requires knowledge of the genetic variability and epidemiology of A. salmonicida. Pathogenic strains were isolated from kidneys, muscles, liver and spleen of rainbow tr...
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BdFISH
2025-02-01
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| Series: | Journal of Fisheries |
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| Online Access: | https://journal.bdfish.org/index.php/fisheries/article/view/683 |
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| author | Muhammad Akram Muhammad Hafeez-ur-Rehman Farzana Abbas Imran Altaf Sidra Kanwal Nimra Mobeen Aiza Khaliq Asma Sharif Maria Tayyaba Saira Talib Muhammad Nouman Riaz Saima Zafar Ikram Hussain Karim Johar Khan Fatima Sughra |
| author_facet | Muhammad Akram Muhammad Hafeez-ur-Rehman Farzana Abbas Imran Altaf Sidra Kanwal Nimra Mobeen Aiza Khaliq Asma Sharif Maria Tayyaba Saira Talib Muhammad Nouman Riaz Saima Zafar Ikram Hussain Karim Johar Khan Fatima Sughra |
| author_sort | Muhammad Akram |
| collection | DOAJ |
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Aeromonas salmonicida causes furunculosis in the Oncorhynchus mykiss (rainbow trout) and the effective control over this infection requires knowledge of the genetic variability and epidemiology of A. salmonicida. Pathogenic strains were isolated from kidneys, muscles, liver and spleen of rainbow trout from the provinces, Punjab, Khyber Pakhtunkhwa and Gilgit Baltistan, Pakistan. On the base of 16S rRNA sequencing, physiological and biochemical characterization, isolated bacterial strains were identified as A. salmonicida (NCBI Ref. ArS-Pak-19 [MW307221], ArS-Pak- GB1-19 [MW720959], ArS-Pak-MRE-19 [MW720960], ArS-Pak-SW2-19 [MW720961], ArS-SW1-Pak-19 [MW720962]). Isolated strains were resistant to antibiotics like sulfamethoxazole, penicillin, vanomycine, rifampicin and bacitracin but were extremely sensitive to spectinomycin, ciprofloxacin, ofloxacin, levofloxacin and nalidixic acid. To check out the pathogenicity, rainbow trout were experimentally infected with isolated strains. Experimental fishes showed the same symptoms as were recorded in naturally infected fish including jaw bleeding, intra-abdominal fluid, intestinal bleeding and gill filament anemia. After ten days of post-challenge study, histopathological analysis revealed that there were severe alterations in the spleen, liver and kidney of the infected fish. The present study provides further research foundation and for upcoming research on A. salmonicida disease, its control and epidemiology.
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| format | Article |
| id | doaj-art-ad0cc9d371f244fda7a1f95f470f0d2a |
| institution | OA Journals |
| issn | 2311-729X 2311-3111 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BdFISH |
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| series | Journal of Fisheries |
| spelling | doaj-art-ad0cc9d371f244fda7a1f95f470f0d2a2025-08-20T02:13:06ZengBdFISHJournal of Fisheries2311-729X2311-31112025-02-0113110.17017/j.fish.683Identification, isolation and pathogenicity of Aeromonas salmonicida and his-topathology of infected Oncorhynchus mykiss in Punjab and northern areas of PakistanMuhammad Akram0Muhammad Hafeez-ur-Rehman1Farzana Abbas2Imran Altaf3Sidra Kanwal4Nimra Mobeen5Aiza Khaliq6Asma Sharif7Maria Tayyaba8Saira Talib9Muhammad Nouman Riaz10Saima Zafar11Ikram Hussain12Karim Johar Khan13Fatima Sughra14Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Punjab 54000, PakistanDepartment of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Punjab 54000, PakistanDepartment of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Punjab 54000, PakistanInstitute of Microbiology, University of Veterinary and Animal Sciences, Lahore, Punjab 54000, PakistanDepartment of Zoology, University of Okara, Punjab 56000, PakistanInstitute of Microbiology, University of Veterinary and Animal Sciences, Lahore, Punjab 54000, PakistanDepartment of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Punjab 54000, PakistanDepartment of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Punjab 54000, PakistanDepartment of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Punjab 54000, PakistanDepartment of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Punjab 54000, PakistanDepartment of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Punjab 54000, PakistanDepartment of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Punjab 54000, PakistanDepartment of Fisheries, Gilgit-Baltistan 15711, PakistanDepartment of Fisheries, Gilgit-Baltistan 15711, PakistanDepartment of Zoology, University of Education, Punjab 54000, Pakistan Aeromonas salmonicida causes furunculosis in the Oncorhynchus mykiss (rainbow trout) and the effective control over this infection requires knowledge of the genetic variability and epidemiology of A. salmonicida. Pathogenic strains were isolated from kidneys, muscles, liver and spleen of rainbow trout from the provinces, Punjab, Khyber Pakhtunkhwa and Gilgit Baltistan, Pakistan. On the base of 16S rRNA sequencing, physiological and biochemical characterization, isolated bacterial strains were identified as A. salmonicida (NCBI Ref. ArS-Pak-19 [MW307221], ArS-Pak- GB1-19 [MW720959], ArS-Pak-MRE-19 [MW720960], ArS-Pak-SW2-19 [MW720961], ArS-SW1-Pak-19 [MW720962]). Isolated strains were resistant to antibiotics like sulfamethoxazole, penicillin, vanomycine, rifampicin and bacitracin but were extremely sensitive to spectinomycin, ciprofloxacin, ofloxacin, levofloxacin and nalidixic acid. To check out the pathogenicity, rainbow trout were experimentally infected with isolated strains. Experimental fishes showed the same symptoms as were recorded in naturally infected fish including jaw bleeding, intra-abdominal fluid, intestinal bleeding and gill filament anemia. After ten days of post-challenge study, histopathological analysis revealed that there were severe alterations in the spleen, liver and kidney of the infected fish. The present study provides further research foundation and for upcoming research on A. salmonicida disease, its control and epidemiology. https://journal.bdfish.org/index.php/fisheries/article/view/68316S rRNAAeromonas salmonicidabiochemical characterizationhistopathologyisolation |
| spellingShingle | Muhammad Akram Muhammad Hafeez-ur-Rehman Farzana Abbas Imran Altaf Sidra Kanwal Nimra Mobeen Aiza Khaliq Asma Sharif Maria Tayyaba Saira Talib Muhammad Nouman Riaz Saima Zafar Ikram Hussain Karim Johar Khan Fatima Sughra Identification, isolation and pathogenicity of Aeromonas salmonicida and his-topathology of infected Oncorhynchus mykiss in Punjab and northern areas of Pakistan Journal of Fisheries 16S rRNA Aeromonas salmonicida biochemical characterization histopathology isolation |
| title | Identification, isolation and pathogenicity of Aeromonas salmonicida and his-topathology of infected Oncorhynchus mykiss in Punjab and northern areas of Pakistan |
| title_full | Identification, isolation and pathogenicity of Aeromonas salmonicida and his-topathology of infected Oncorhynchus mykiss in Punjab and northern areas of Pakistan |
| title_fullStr | Identification, isolation and pathogenicity of Aeromonas salmonicida and his-topathology of infected Oncorhynchus mykiss in Punjab and northern areas of Pakistan |
| title_full_unstemmed | Identification, isolation and pathogenicity of Aeromonas salmonicida and his-topathology of infected Oncorhynchus mykiss in Punjab and northern areas of Pakistan |
| title_short | Identification, isolation and pathogenicity of Aeromonas salmonicida and his-topathology of infected Oncorhynchus mykiss in Punjab and northern areas of Pakistan |
| title_sort | identification isolation and pathogenicity of aeromonas salmonicida and his topathology of infected oncorhynchus mykiss in punjab and northern areas of pakistan |
| topic | 16S rRNA Aeromonas salmonicida biochemical characterization histopathology isolation |
| url | https://journal.bdfish.org/index.php/fisheries/article/view/683 |
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