Multifunctional 4D-printed redox-responsive needle panel meter employing geometric changes for glucose and lactate concentration measurements
Most biosensors routinely used for the detection of body metabolites commonly suffer from low patient compliance due to invasive sampling, high cost, and lack of portability for point-of-care (POC) diagnostic applications. Thus, we aimed to fabricate robust, user-friendly, affordable, and redox-resp...
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Elsevier
2025-07-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127525006483 |
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| author | Tzu-Hsuan Yang Yihenew Simegniew Birhan Po-Cheng Chu Leimapokpam Romina Chanu Ping-Shan Lai |
| author_facet | Tzu-Hsuan Yang Yihenew Simegniew Birhan Po-Cheng Chu Leimapokpam Romina Chanu Ping-Shan Lai |
| author_sort | Tzu-Hsuan Yang |
| collection | DOAJ |
| description | Most biosensors routinely used for the detection of body metabolites commonly suffer from low patient compliance due to invasive sampling, high cost, and lack of portability for point-of-care (POC) diagnostic applications. Thus, we aimed to fabricate robust, user-friendly, affordable, and redox-responsive four-dimensional (4D)-printed needle panel meter (NPM) from 2,2ꞌ-(Ethylenedioxy)diethanethiol (EDDT) and Phrozen resin (PR) for glucose (GLU) and lactate (LAC) detection from blood, sweat and urine samples. Optimal needle bending was noticed upon the addition of target analytes into the NPM containing glucose oxidase (GOx, 5 U/mL, pH = 5.0, 20 min) or lactate oxidase (LOx, 0.01 U/mL, pH = 7.0, 45 min) and reversed back when reduced with NaBH4 (1.25 wt%). To ensure its feasibility and equitable distribution in underprivileged sub-Saharan African countries (SSACs), three sets of concentration scale marks analogous to the temperature ranges of 20-26℃, 27-33℃, and 34-40℃ were adopted. The NPM precisely measured significantly low concentrations of GLU and LAC in high-salt matrix samples with considerably low limits of detection (LOD): 0.21 and 0.11 µM, respectively. In conclusion, the reusability, storage stability, and ease of needle maintenance make the NPM desirable for the quantitative determination of GLU and LAC from complex biological samples. |
| format | Article |
| id | doaj-art-ad0b2da71c3541668cab469ca06e9f83 |
| institution | OA Journals |
| issn | 0264-1275 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj-art-ad0b2da71c3541668cab469ca06e9f832025-08-20T02:10:01ZengElsevierMaterials & Design0264-12752025-07-0125511422810.1016/j.matdes.2025.114228Multifunctional 4D-printed redox-responsive needle panel meter employing geometric changes for glucose and lactate concentration measurementsTzu-Hsuan Yang0Yihenew Simegniew Birhan1Po-Cheng Chu2Leimapokpam Romina Chanu3Ping-Shan Lai4Department of Chemistry, National Chung Hsing University, Taichung 402, TaiwanDepartment of Chemistry, National Chung Hsing University, Taichung 402, Taiwan; Department of Chemistry, College of Natural and Computational Sciences, Debre Markos University, P.O. Box 269, Debre Markos, EthiopiaDepartment of Chemistry, National Chung Hsing University, Taichung 402, Taiwan; Basic Research and Development Department, Powin Biomedical Co. Ltd., Taichung 428, TaiwanPh.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, Taichung 402, TaiwanDepartment of Chemistry, National Chung Hsing University, Taichung 402, Taiwan; Ph.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, Taichung 402, Taiwan; Corresponding author at: Department of Chemistry, National Chung Hsing University, Taichung 402, Taiwan.Most biosensors routinely used for the detection of body metabolites commonly suffer from low patient compliance due to invasive sampling, high cost, and lack of portability for point-of-care (POC) diagnostic applications. Thus, we aimed to fabricate robust, user-friendly, affordable, and redox-responsive four-dimensional (4D)-printed needle panel meter (NPM) from 2,2ꞌ-(Ethylenedioxy)diethanethiol (EDDT) and Phrozen resin (PR) for glucose (GLU) and lactate (LAC) detection from blood, sweat and urine samples. Optimal needle bending was noticed upon the addition of target analytes into the NPM containing glucose oxidase (GOx, 5 U/mL, pH = 5.0, 20 min) or lactate oxidase (LOx, 0.01 U/mL, pH = 7.0, 45 min) and reversed back when reduced with NaBH4 (1.25 wt%). To ensure its feasibility and equitable distribution in underprivileged sub-Saharan African countries (SSACs), three sets of concentration scale marks analogous to the temperature ranges of 20-26℃, 27-33℃, and 34-40℃ were adopted. The NPM precisely measured significantly low concentrations of GLU and LAC in high-salt matrix samples with considerably low limits of detection (LOD): 0.21 and 0.11 µM, respectively. In conclusion, the reusability, storage stability, and ease of needle maintenance make the NPM desirable for the quantitative determination of GLU and LAC from complex biological samples.http://www.sciencedirect.com/science/article/pii/S02641275250064834D printingGlucoseLactateBiosensorsNeedle panel metersRedox-responsive |
| spellingShingle | Tzu-Hsuan Yang Yihenew Simegniew Birhan Po-Cheng Chu Leimapokpam Romina Chanu Ping-Shan Lai Multifunctional 4D-printed redox-responsive needle panel meter employing geometric changes for glucose and lactate concentration measurements Materials & Design 4D printing Glucose Lactate Biosensors Needle panel meters Redox-responsive |
| title | Multifunctional 4D-printed redox-responsive needle panel meter employing geometric changes for glucose and lactate concentration measurements |
| title_full | Multifunctional 4D-printed redox-responsive needle panel meter employing geometric changes for glucose and lactate concentration measurements |
| title_fullStr | Multifunctional 4D-printed redox-responsive needle panel meter employing geometric changes for glucose and lactate concentration measurements |
| title_full_unstemmed | Multifunctional 4D-printed redox-responsive needle panel meter employing geometric changes for glucose and lactate concentration measurements |
| title_short | Multifunctional 4D-printed redox-responsive needle panel meter employing geometric changes for glucose and lactate concentration measurements |
| title_sort | multifunctional 4d printed redox responsive needle panel meter employing geometric changes for glucose and lactate concentration measurements |
| topic | 4D printing Glucose Lactate Biosensors Needle panel meters Redox-responsive |
| url | http://www.sciencedirect.com/science/article/pii/S0264127525006483 |
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