Glyphosate bioremediation using a newly isolated Bacillus albus strain F9D: mechanisms and kinetic studies
Abstract Glyphosate is widely used as an herbicide around the world. The extensive application of glyphosate, however, has serious adverse effects on living systems. Therefore, the elimination of residual glyphosate pollution has become an urgent issue worldwide. In the present study, a novel bacter...
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BMC
2025-08-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-025-02758-1 |
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| author | Wen-Juan Chen Mingqiu Liu Shao-Fang Chen Yuming Zhang Haoran Song Maman Hassan Abdoulahi Kalpana Bhatt Sandhya Mishra Mohamed A. Ghorab Wenping Zhang Shaohua Chen |
| author_facet | Wen-Juan Chen Mingqiu Liu Shao-Fang Chen Yuming Zhang Haoran Song Maman Hassan Abdoulahi Kalpana Bhatt Sandhya Mishra Mohamed A. Ghorab Wenping Zhang Shaohua Chen |
| author_sort | Wen-Juan Chen |
| collection | DOAJ |
| description | Abstract Glyphosate is widely used as an herbicide around the world. The extensive application of glyphosate, however, has serious adverse effects on living systems. Therefore, the elimination of residual glyphosate pollution has become an urgent issue worldwide. In the present study, a novel bacterial strain named F9D was identified as Bacillus albus, based on its physio-biochemical characteristics and 16 S rDNA analysis. This strain can completely degrade glyphosate (400 mg/L) within 5 days. An effective, rapid, and stable detection method for glyphosate and aminomethylphosphonic acid (AMPA) was developed using ultra-performance liquid chromatography–tandem mass spectrometry technology (UPLC-MS/MS). The degradability of glyphosate by the degrading strain F9D was optimized, considering various conditions, as follows: initial pH (5–9), incubation temperature (20–40℃), glyphosate concentration (50–800 mg/L), and inoculation amount (1–5%). The strain also demonstrated strong degradation ability in soil and water–sediment systems: 78.1% glyphosate (400 mg/kg) and 83.2% glyphosate (200 mg/kg), respectively, degraded in soil and water–sediment systems within 5 days of incubation. Furthermore, the F9D strain is capable of degrading 50–800 mg/L of glyphosate and AMPA under various treatments. Hence, the notable ability of B. albus strain F9D to degrade glyphosate makes it a highly promising candidate for the removal of this emerging contaminant from the environment on a large scale. |
| format | Article |
| id | doaj-art-acf1889e2a464837baba64e3d44885d4 |
| institution | Kabale University |
| issn | 1475-2859 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj-art-acf1889e2a464837baba64e3d44885d42025-08-24T12:02:13ZengBMCMicrobial Cell Factories1475-28592025-08-0124111510.1186/s12934-025-02758-1Glyphosate bioremediation using a newly isolated Bacillus albus strain F9D: mechanisms and kinetic studiesWen-Juan Chen0Mingqiu Liu1Shao-Fang Chen2Yuming Zhang3Haoran Song4Maman Hassan Abdoulahi5Kalpana Bhatt6Sandhya Mishra7Mohamed A. Ghorab8Wenping Zhang9Shaohua Chen10State Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityIntegrative Microbiology Research Centre, South China Agricultural UniversityWildlife Toxicology Lab, Department of Animal Science, Institute for Integrative Toxicology (IIT), Michigan State UniversityState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, College of Plant Protection, South China Agricultural UniversityIntegrative Microbiology Research Centre, South China Agricultural UniversityAbstract Glyphosate is widely used as an herbicide around the world. The extensive application of glyphosate, however, has serious adverse effects on living systems. Therefore, the elimination of residual glyphosate pollution has become an urgent issue worldwide. In the present study, a novel bacterial strain named F9D was identified as Bacillus albus, based on its physio-biochemical characteristics and 16 S rDNA analysis. This strain can completely degrade glyphosate (400 mg/L) within 5 days. An effective, rapid, and stable detection method for glyphosate and aminomethylphosphonic acid (AMPA) was developed using ultra-performance liquid chromatography–tandem mass spectrometry technology (UPLC-MS/MS). The degradability of glyphosate by the degrading strain F9D was optimized, considering various conditions, as follows: initial pH (5–9), incubation temperature (20–40℃), glyphosate concentration (50–800 mg/L), and inoculation amount (1–5%). The strain also demonstrated strong degradation ability in soil and water–sediment systems: 78.1% glyphosate (400 mg/kg) and 83.2% glyphosate (200 mg/kg), respectively, degraded in soil and water–sediment systems within 5 days of incubation. Furthermore, the F9D strain is capable of degrading 50–800 mg/L of glyphosate and AMPA under various treatments. Hence, the notable ability of B. albus strain F9D to degrade glyphosate makes it a highly promising candidate for the removal of this emerging contaminant from the environment on a large scale.https://doi.org/10.1186/s12934-025-02758-1GlyphosateBacillus albusBiodegradationKineticsBiochemical pathwaysSoil remediation |
| spellingShingle | Wen-Juan Chen Mingqiu Liu Shao-Fang Chen Yuming Zhang Haoran Song Maman Hassan Abdoulahi Kalpana Bhatt Sandhya Mishra Mohamed A. Ghorab Wenping Zhang Shaohua Chen Glyphosate bioremediation using a newly isolated Bacillus albus strain F9D: mechanisms and kinetic studies Microbial Cell Factories Glyphosate Bacillus albus Biodegradation Kinetics Biochemical pathways Soil remediation |
| title | Glyphosate bioremediation using a newly isolated Bacillus albus strain F9D: mechanisms and kinetic studies |
| title_full | Glyphosate bioremediation using a newly isolated Bacillus albus strain F9D: mechanisms and kinetic studies |
| title_fullStr | Glyphosate bioremediation using a newly isolated Bacillus albus strain F9D: mechanisms and kinetic studies |
| title_full_unstemmed | Glyphosate bioremediation using a newly isolated Bacillus albus strain F9D: mechanisms and kinetic studies |
| title_short | Glyphosate bioremediation using a newly isolated Bacillus albus strain F9D: mechanisms and kinetic studies |
| title_sort | glyphosate bioremediation using a newly isolated bacillus albus strain f9d mechanisms and kinetic studies |
| topic | Glyphosate Bacillus albus Biodegradation Kinetics Biochemical pathways Soil remediation |
| url | https://doi.org/10.1186/s12934-025-02758-1 |
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