Modeling of femoral head aseptic necrosis in Wistar rats
Aim. To develop an animal model of femoral head aseptic necrosis for studying Legg–Calvé–Perthes disease.Materials and Methods. To induce the development of aseptic necrosis, we used Wistar rats (n = 8) which suffered from combined hypoperfusion of the femoral head and increased intra-articular pres...
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| Language: | Russian |
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Kemerovo State Medical University
2022-10-01
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| Series: | Фундаментальная и клиническая медицина |
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| Online Access: | https://fcm.kemsmu.ru/jour/article/view/569 |
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| author | N. A. Shabaldin A. V. Shabaldin N. E. Popova A. V. Postnikova L. A. Bogdanov A. V. Tsepokina |
| author_facet | N. A. Shabaldin A. V. Shabaldin N. E. Popova A. V. Postnikova L. A. Bogdanov A. V. Tsepokina |
| author_sort | N. A. Shabaldin |
| collection | DOAJ |
| description | Aim. To develop an animal model of femoral head aseptic necrosis for studying Legg–Calvé–Perthes disease.Materials and Methods. To induce the development of aseptic necrosis, we used Wistar rats (n = 8) which suffered from combined hypoperfusion of the femoral head and increased intra-articular pressure in the hip joint. Having employed isoflurane anesthesia, we performed an incision (≈ 3 cm length) on the outer surface of the thigh in the projection of the hip joint and then excised periosteum in the proximal third of the femur. A dense vicryl ligature was applied around the femoral neck to reduce blood perfusion of the femoral head. Further, 1.5 mL 2% rheopolyglucinum solution (10% isotonic dextran, 30-40 kDa molecular weight) was injected into the hip joint cavity to increase intra-articular pressure. Rats were sacrificed upon 8-week follow-up with subsequent X-ray and histological examination.Results. Our animal model of femoral head aseptic necrosis includes two main components of Legg–Calvé–Perthes disease: an increase in the intra-articular pressure and insufficient blood perfusion of the femoral head. In all (8/8) cases, aseptic necrosis of the femoral head was achieved. Eight weeks post intervention, the condition of the proximal femur 8 was similar to impression fracture.Conclusion. Our model of femoral head aseptic necrosis fully reflects the pathogenesis of LeggCalve-Perthes disease and can be therefore used in experimental studies. |
| format | Article |
| id | doaj-art-acefb72c45394a79b31feb67f2362d9d |
| institution | DOAJ |
| issn | 2500-0764 2542-0941 |
| language | Russian |
| publishDate | 2022-10-01 |
| publisher | Kemerovo State Medical University |
| record_format | Article |
| series | Фундаментальная и клиническая медицина |
| spelling | doaj-art-acefb72c45394a79b31feb67f2362d9d2025-08-20T02:49:12ZrusKemerovo State Medical UniversityФундаментальная и клиническая медицина2500-07642542-09412022-10-0173233010.23946/2500-0764-2022-7-3-23-30326Modeling of femoral head aseptic necrosis in Wistar ratsN. A. Shabaldin0A. V. Shabaldin1N. E. Popova2A. V. Postnikova3L. A. Bogdanov4A. V. Tsepokina5Kemerovo State Medical UniversityKemerovo State Medical University; Research Institute for Complex Issues of Cardiovascular DiseasesKemerovo State Medical UniversityKemerovo State Medical UniversityResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesAim. To develop an animal model of femoral head aseptic necrosis for studying Legg–Calvé–Perthes disease.Materials and Methods. To induce the development of aseptic necrosis, we used Wistar rats (n = 8) which suffered from combined hypoperfusion of the femoral head and increased intra-articular pressure in the hip joint. Having employed isoflurane anesthesia, we performed an incision (≈ 3 cm length) on the outer surface of the thigh in the projection of the hip joint and then excised periosteum in the proximal third of the femur. A dense vicryl ligature was applied around the femoral neck to reduce blood perfusion of the femoral head. Further, 1.5 mL 2% rheopolyglucinum solution (10% isotonic dextran, 30-40 kDa molecular weight) was injected into the hip joint cavity to increase intra-articular pressure. Rats were sacrificed upon 8-week follow-up with subsequent X-ray and histological examination.Results. Our animal model of femoral head aseptic necrosis includes two main components of Legg–Calvé–Perthes disease: an increase in the intra-articular pressure and insufficient blood perfusion of the femoral head. In all (8/8) cases, aseptic necrosis of the femoral head was achieved. Eight weeks post intervention, the condition of the proximal femur 8 was similar to impression fracture.Conclusion. Our model of femoral head aseptic necrosis fully reflects the pathogenesis of LeggCalve-Perthes disease and can be therefore used in experimental studies.https://fcm.kemsmu.ru/jour/article/view/569model experimentaseptic necrosislegg-calve-perthes disease |
| spellingShingle | N. A. Shabaldin A. V. Shabaldin N. E. Popova A. V. Postnikova L. A. Bogdanov A. V. Tsepokina Modeling of femoral head aseptic necrosis in Wistar rats Фундаментальная и клиническая медицина model experiment aseptic necrosis legg-calve-perthes disease |
| title | Modeling of femoral head aseptic necrosis in Wistar rats |
| title_full | Modeling of femoral head aseptic necrosis in Wistar rats |
| title_fullStr | Modeling of femoral head aseptic necrosis in Wistar rats |
| title_full_unstemmed | Modeling of femoral head aseptic necrosis in Wistar rats |
| title_short | Modeling of femoral head aseptic necrosis in Wistar rats |
| title_sort | modeling of femoral head aseptic necrosis in wistar rats |
| topic | model experiment aseptic necrosis legg-calve-perthes disease |
| url | https://fcm.kemsmu.ru/jour/article/view/569 |
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