Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology
Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful technique for identification of microorganisms, changing the workflow of well-established laboratories so that its impact on microbiological diagnostics has been unparallele...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2014-09-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/3623 |
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| author | Elena De Carolis Antonietta Vella Luisa Vaccaro Riccardo Torelli Teresa Spanu Barbara Fiori Brunella Posteraro Maurizio Sanguinetti |
| author_facet | Elena De Carolis Antonietta Vella Luisa Vaccaro Riccardo Torelli Teresa Spanu Barbara Fiori Brunella Posteraro Maurizio Sanguinetti |
| author_sort | Elena De Carolis |
| collection | DOAJ |
| description | Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful technique for identification of microorganisms, changing the workflow of well-established laboratories so that its impact on microbiological diagnostics has been unparalleled.
In comparison with conventional identification methods that rely on biochemical tests and require long incubation procedures, MALDI-TOF MS has the advantage of identifying bacteria and fungi directly from colonies grown on culture plates in a few minutes and with simple procedures.
Numerous studies on different systems available demonstrate the reliability and accuracy of the method, and new frontiers have been explored besides microbial species level identification, such as direct identification of pathogens from positive blood cultures, subtyping, and drug susceptibility detection.
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| format | Article |
| id | doaj-art-aced8cc409e64f529f443898483e6a17 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2014-09-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-aced8cc409e64f529f443898483e6a172025-08-20T02:14:10ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802014-09-0180910.3855/jidc.3623Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiologyElena De Carolis0Antonietta Vella1Luisa Vaccaro2Riccardo Torelli3Teresa Spanu4Barbara Fiori5Brunella Posteraro6Maurizio Sanguinetti7Catholic University of the Sacred Hearth, Rome, ItalyCatholic University of the Sacred Hearth, Rome, ItalyCatholic University of the Sacred Hearth, Rome, ItalyCatholic University of the Sacred Hearth, Rome, ItalyCatholic University of the Sacred Hearth, Rome, ItalyCatholic University of the Sacred Hearth, Rome, ItalyCatholic University of the Sacred Hearth, Rome, ItalyCatholic University of the Sacred Hearth, Rome, ItalyMatrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful technique for identification of microorganisms, changing the workflow of well-established laboratories so that its impact on microbiological diagnostics has been unparalleled. In comparison with conventional identification methods that rely on biochemical tests and require long incubation procedures, MALDI-TOF MS has the advantage of identifying bacteria and fungi directly from colonies grown on culture plates in a few minutes and with simple procedures. Numerous studies on different systems available demonstrate the reliability and accuracy of the method, and new frontiers have been explored besides microbial species level identification, such as direct identification of pathogens from positive blood cultures, subtyping, and drug susceptibility detection. https://jidc.org/index.php/journal/article/view/3623MALDI-TOF MSdiagnostic microbiologysubtypingantimicrobial resistance |
| spellingShingle | Elena De Carolis Antonietta Vella Luisa Vaccaro Riccardo Torelli Teresa Spanu Barbara Fiori Brunella Posteraro Maurizio Sanguinetti Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology Journal of Infection in Developing Countries MALDI-TOF MS diagnostic microbiology subtyping antimicrobial resistance |
| title | Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology |
| title_full | Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology |
| title_fullStr | Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology |
| title_full_unstemmed | Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology |
| title_short | Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology |
| title_sort | application of maldi tof mass spectrometry in clinical diagnostic microbiology |
| topic | MALDI-TOF MS diagnostic microbiology subtyping antimicrobial resistance |
| url | https://jidc.org/index.php/journal/article/view/3623 |
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