Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural Tuberculosis
Aim: to evaluate the diagnostic value of an interferon-gamma release assay (IGRA) with enzyme-linked immunospot (ELISPOT) method, T-SPOT.TB, in the diagnosis of pleural TB using pleural fluid mononuclear cells (PFMC). Methods: forty-eight subjects, presumed to have pleural TB with exudative pleural...
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| Language: | English |
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Interna Publishing
2016-09-01
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| Series: | Acta Medica Indonesiana |
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| Online Access: | https://actamedindones.org/index.php/ijim/article/view/136 |
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| author | Tika Adilistya Dalima AW Astrawinata Ujainah Z Nasir |
| author_facet | Tika Adilistya Dalima AW Astrawinata Ujainah Z Nasir |
| author_sort | Tika Adilistya |
| collection | DOAJ |
| description | Aim: to evaluate the diagnostic value of an interferon-gamma release assay (IGRA) with enzyme-linked immunospot (ELISPOT) method, T-SPOT.TB, in the diagnosis of pleural TB using pleural fluid mononuclear cells (PFMC). Methods: forty-eight subjects, presumed to have pleural TB with exudative pleural effusion by Light’s criteria, dominated by mononuclear cells, had their pleural fluid specimen tested with T-SPOT.TB, Mycobacterial Growth Indicator Tube (MGIT) culture, and adenosine deaminase (ADA) activity. Other causes of pleural effusion such as heart failure, renal failure, hepatic cirrhosis, and malignancy were excluded.
Results: the sensitivity, specificity, positive predictive value, and negative predictive value of the IGRA ELISPOT assay using PFMC for the diagnosis of pleural TB were 100%, 88.89%, 97.5%, and 100%, respectively. Conclusion: IGRA with ELISPOT method performed on PFMC is useful for a rapid and reliable diagnosis of pleural TB in clinical practice, especially in area with high TB burden.
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| format | Article |
| id | doaj-art-acec780d34fe4ae6818b7d3e0217d4d0 |
| institution | DOAJ |
| issn | 0125-9326 2338-2732 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | Interna Publishing |
| record_format | Article |
| series | Acta Medica Indonesiana |
| spelling | doaj-art-acec780d34fe4ae6818b7d3e0217d4d02025-08-20T02:50:49ZengInterna PublishingActa Medica Indonesiana0125-93262338-27322016-09-01481Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural TuberculosisTika Adilistya0Dalima AW Astrawinata1Ujainah Z Nasir2Department of Clinical Pathology, Cipto Mangunkusumo Hospital, Jakarta, IndonesiaDepartment of Clinical Pathology, Cipto Mangunkusumo Hospital, Jakarta, IndonesiaDepartment of Clinical Pathology, Cipto Mangunkusumo Hospital, Jakarta, IndonesiaAim: to evaluate the diagnostic value of an interferon-gamma release assay (IGRA) with enzyme-linked immunospot (ELISPOT) method, T-SPOT.TB, in the diagnosis of pleural TB using pleural fluid mononuclear cells (PFMC). Methods: forty-eight subjects, presumed to have pleural TB with exudative pleural effusion by Light’s criteria, dominated by mononuclear cells, had their pleural fluid specimen tested with T-SPOT.TB, Mycobacterial Growth Indicator Tube (MGIT) culture, and adenosine deaminase (ADA) activity. Other causes of pleural effusion such as heart failure, renal failure, hepatic cirrhosis, and malignancy were excluded. Results: the sensitivity, specificity, positive predictive value, and negative predictive value of the IGRA ELISPOT assay using PFMC for the diagnosis of pleural TB were 100%, 88.89%, 97.5%, and 100%, respectively. Conclusion: IGRA with ELISPOT method performed on PFMC is useful for a rapid and reliable diagnosis of pleural TB in clinical practice, especially in area with high TB burden. https://actamedindones.org/index.php/ijim/article/view/136interferon-γ release assayELISPOTpleural tuberculosis |
| spellingShingle | Tika Adilistya Dalima AW Astrawinata Ujainah Z Nasir Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural Tuberculosis Acta Medica Indonesiana interferon-γ release assay ELISPOT pleural tuberculosis |
| title | Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural Tuberculosis |
| title_full | Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural Tuberculosis |
| title_fullStr | Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural Tuberculosis |
| title_full_unstemmed | Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural Tuberculosis |
| title_short | Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural Tuberculosis |
| title_sort | use of pleural fluid interferon gamma enzyme linked immunospot assay in the diagnosis of pleural tuberculosis |
| topic | interferon-γ release assay ELISPOT pleural tuberculosis |
| url | https://actamedindones.org/index.php/ijim/article/view/136 |
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