Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural Tuberculosis
Aim: to evaluate the diagnostic value of an interferon-gamma release assay (IGRA) with enzyme-linked immunospot (ELISPOT) method, T-SPOT.TB, in the diagnosis of pleural TB using pleural fluid mononuclear cells (PFMC). Methods: forty-eight subjects, presumed to have pleural TB with exudative pleural...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Interna Publishing
2016-09-01
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| Series: | Acta Medica Indonesiana |
| Subjects: | |
| Online Access: | https://actamedindones.org/index.php/ijim/article/view/136 |
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| Summary: | Aim: to evaluate the diagnostic value of an interferon-gamma release assay (IGRA) with enzyme-linked immunospot (ELISPOT) method, T-SPOT.TB, in the diagnosis of pleural TB using pleural fluid mononuclear cells (PFMC). Methods: forty-eight subjects, presumed to have pleural TB with exudative pleural effusion by Light’s criteria, dominated by mononuclear cells, had their pleural fluid specimen tested with T-SPOT.TB, Mycobacterial Growth Indicator Tube (MGIT) culture, and adenosine deaminase (ADA) activity. Other causes of pleural effusion such as heart failure, renal failure, hepatic cirrhosis, and malignancy were excluded.
Results: the sensitivity, specificity, positive predictive value, and negative predictive value of the IGRA ELISPOT assay using PFMC for the diagnosis of pleural TB were 100%, 88.89%, 97.5%, and 100%, respectively. Conclusion: IGRA with ELISPOT method performed on PFMC is useful for a rapid and reliable diagnosis of pleural TB in clinical practice, especially in area with high TB burden.
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| ISSN: | 0125-9326 2338-2732 |