Study of interaction between extracellular matrix proteins and receptors of CD133+ stem cells and CD133– differentiated glioma cells
Background. Treatment of glioblastoma multiforme remains little effective due to the rapidly developing recurrence of the tumor, due to its high tumorigenic potential, resistance to chemoradiation therapy and increased dissemination of glioma stem cells (GSC). Molecular mechanisms of these cell inte...
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| Format: | Article |
| Language: | Russian |
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ABV-press
2019-04-01
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| Series: | Успехи молекулярной онкологии |
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| Online Access: | https://umo.abvpress.ru/jour/article/view/208 |
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| author | V. E. Shevchenko I. S. Bryukhovetskiy E. A. Savchenko N. E. Arnotskaya |
| author_facet | V. E. Shevchenko I. S. Bryukhovetskiy E. A. Savchenko N. E. Arnotskaya |
| author_sort | V. E. Shevchenko |
| collection | DOAJ |
| description | Background. Treatment of glioblastoma multiforme remains little effective due to the rapidly developing recurrence of the tumor, due to its high tumorigenic potential, resistance to chemoradiation therapy and increased dissemination of glioma stem cells (GSC). Molecular mechanisms of these cell interaction with extracellular matrix (ECM) are practically not studied. At present, it is also not clear the signaling of the ECM-receptor interaction (ECM-RI) differs for GSC and differentiated glioma cells (GDC).Objective: using high-resolution proteomic mass spectrometry to study the determinant expression of the ECM-receptor interaction signaling cascade in CD133+ GSC and CD133– GDC.Results. 1990 proteins are identified, 18 of which are associated with the ECM-RI process. Positive regulation of 14 ECM-RI proteins was found in CD133+ GSC compared with CD133– GDC, ten had more than 2 times increased expression. Increase in the CD133+ GSC level of 4 proteins activating the ECM-RI signaling cascade was noted.Conclusion. Important regularities are determined that could be used for the development of new approaches for detection of potential therapy targets of glioblastoma multiforme. |
| format | Article |
| id | doaj-art-acec70657b044d9a9b8f28cdb6ef069b |
| institution | Kabale University |
| issn | 2313-805X 2413-3787 |
| language | Russian |
| publishDate | 2019-04-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Успехи молекулярной онкологии |
| spelling | doaj-art-acec70657b044d9a9b8f28cdb6ef069b2025-08-20T03:37:43ZrusABV-pressУспехи молекулярной онкологии2313-805X2413-37872019-04-0161637210.17650/2313-805X-2019-6-1-63-72157Study of interaction between extracellular matrix proteins and receptors of CD133+ stem cells and CD133– differentiated glioma cellsV. E. Shevchenko0I. S. Bryukhovetskiy1E. A. Savchenko2N. E. Arnotskaya3Research Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of RussiaSchool of Biomedicine, Far Eastern Federal University; National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of SciencesLimited Liability Company “APTO-FARM”Research Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of RussiaBackground. Treatment of glioblastoma multiforme remains little effective due to the rapidly developing recurrence of the tumor, due to its high tumorigenic potential, resistance to chemoradiation therapy and increased dissemination of glioma stem cells (GSC). Molecular mechanisms of these cell interaction with extracellular matrix (ECM) are practically not studied. At present, it is also not clear the signaling of the ECM-receptor interaction (ECM-RI) differs for GSC and differentiated glioma cells (GDC).Objective: using high-resolution proteomic mass spectrometry to study the determinant expression of the ECM-receptor interaction signaling cascade in CD133+ GSC and CD133– GDC.Results. 1990 proteins are identified, 18 of which are associated with the ECM-RI process. Positive regulation of 14 ECM-RI proteins was found in CD133+ GSC compared with CD133– GDC, ten had more than 2 times increased expression. Increase in the CD133+ GSC level of 4 proteins activating the ECM-RI signaling cascade was noted.Conclusion. Important regularities are determined that could be used for the development of new approaches for detection of potential therapy targets of glioblastoma multiforme.https://umo.abvpress.ru/jour/article/view/208extracellular matrixglioma stem cellsglioblastoma multiformeproteomemass-spectrometry |
| spellingShingle | V. E. Shevchenko I. S. Bryukhovetskiy E. A. Savchenko N. E. Arnotskaya Study of interaction between extracellular matrix proteins and receptors of CD133+ stem cells and CD133– differentiated glioma cells Успехи молекулярной онкологии extracellular matrix glioma stem cells glioblastoma multiforme proteome mass-spectrometry |
| title | Study of interaction between extracellular matrix proteins and receptors of CD133+ stem cells and CD133– differentiated glioma cells |
| title_full | Study of interaction between extracellular matrix proteins and receptors of CD133+ stem cells and CD133– differentiated glioma cells |
| title_fullStr | Study of interaction between extracellular matrix proteins and receptors of CD133+ stem cells and CD133– differentiated glioma cells |
| title_full_unstemmed | Study of interaction between extracellular matrix proteins and receptors of CD133+ stem cells and CD133– differentiated glioma cells |
| title_short | Study of interaction between extracellular matrix proteins and receptors of CD133+ stem cells and CD133– differentiated glioma cells |
| title_sort | study of interaction between extracellular matrix proteins and receptors of cd133 stem cells and cd133 differentiated glioma cells |
| topic | extracellular matrix glioma stem cells glioblastoma multiforme proteome mass-spectrometry |
| url | https://umo.abvpress.ru/jour/article/view/208 |
| work_keys_str_mv | AT veshevchenko studyofinteractionbetweenextracellularmatrixproteinsandreceptorsofcd133stemcellsandcd133differentiatedgliomacells AT isbryukhovetskiy studyofinteractionbetweenextracellularmatrixproteinsandreceptorsofcd133stemcellsandcd133differentiatedgliomacells AT easavchenko studyofinteractionbetweenextracellularmatrixproteinsandreceptorsofcd133stemcellsandcd133differentiatedgliomacells AT nearnotskaya studyofinteractionbetweenextracellularmatrixproteinsandreceptorsofcd133stemcellsandcd133differentiatedgliomacells |