Clinical metagenomics analysis of bacterial and fungal microbiota from sputum of patients suspected with tuberculosis infection based on nanopore sequencing

Clinical metagenomics analysis of bacterial and fungal microbiota from sputum of patients suspected with tuberculosis infection based on nanopore sequencing

Abstract Tuberculosis (TB) remains a significant global health challenge, demanding rapid and comprehensive diagnostics for effective treatment. Secondary infections further complicate TB infection, worsening outcomes. Conventional diagnostics are hindered by prolonged turnaround times, high costs,...

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Main Authors: Pawarisa Terbtothakun, Suthida Visedthorn, Pavit Klomkliew, Prangwalai Chanchaem, Vorthon Sawaswong, Pavaret Sivapornnukul, Samitanan Sunantawanit, Ariya Khamwut, Suwatchareeporn Rotcheewaphan, Pornchai Kaewsapsak, Sunchai Payungporn
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
Subjects:
Mycobacterium tuberculosis
Metagenomics
16S rDNA
Internal transcribed spacer (ITS)
Oxford nanopore technologies (ONT)
Online Access:https://doi.org/10.1038/s41598-025-01905-3
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