High-Throughput Determination of Multiclass Chemical Hazards in Poultry Muscles and Eggs Using UPLC–MS/MS
A high-throughput method for the determination of a variety of chemical hazards in poultry muscle and egg samples was established via ultra-performance liquid chromatography–tandem triple quadrupole mass spectrometry (UPLC–QqQ-MS). The sample preparation procedure was developed based on this quick,...
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MDPI AG
2025-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/10/1660 |
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| author | Rong Chen Lan Chen Mingyue Du Qiaozhen Guo Ciping Zhong Jing Zhang Xiaoqin Yu |
| author_facet | Rong Chen Lan Chen Mingyue Du Qiaozhen Guo Ciping Zhong Jing Zhang Xiaoqin Yu |
| author_sort | Rong Chen |
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| description | A high-throughput method for the determination of a variety of chemical hazards in poultry muscle and egg samples was established via ultra-performance liquid chromatography–tandem triple quadrupole mass spectrometry (UPLC–QqQ-MS). The sample preparation procedure was developed based on this quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and validated for 280 chemical hazards potentially present in poultry products. The target compounds in poultry samples were extracted with a 1% formic acid–acetonitrile solution (15:85, <i>v</i>/<i>v</i>), and the metal ions in the matrix were chelated by adding ethylenediaminetetraacetic acid disodium salt (Na<sub>2</sub>EDTA). The supernatant was purified using Enhanced Matrix Removal (EMR) lipid sorbent. Chromatographic gradient separation was performed on an ACQUITY UPLC BEH C18 (2.1 mm × 100 mm, 1.7 μm) column with multiple reaction monitoring (MRM) under both negative- and positive-ion mode. Internal standard calibration or matrix-matched calibration was used for the quantitation. The results showed that good linearity was achieved for each target compound with correlation coefficients (R<sup>2</sup>) ≥ 0.99. The limits of detection (LODs) ranged from 0.05 to 10 µg/kg, and the acceptable limits of quantification (LOQs) were determined to be 0.1–20 µg/kg for all 280 compounds. Approximately 90% of the target compounds exhibited mean recoveries ranging from 60% to 120%, with relative standard deviations (RSDs) within 16.2%. This method can be used for the high-throughput rapid detection of prohibited drug residues in poultry eggs due to its easy operation and high accuracy. It was applied in real sample detection, and 43 chemicals including metronidazole were found in 211 poultry samples, with a concentration range of 0.11–638 μg/kg. |
| format | Article |
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| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-ace60415169249d4af611bc64b8b65952025-08-20T03:47:57ZengMDPI AGFoods2304-81582025-05-011410166010.3390/foods14101660High-Throughput Determination of Multiclass Chemical Hazards in Poultry Muscles and Eggs Using UPLC–MS/MSRong Chen0Lan Chen1Mingyue Du2Qiaozhen Guo3Ciping Zhong4Jing Zhang5Xiaoqin Yu6Key Laboratory of Liquor Regulation Technology, State Administration for Market Regulation, Sichuan Institute of Food Inspection, No. 8 Xinwen Road, West Hi-Tech Zone, Chengdu 611731, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, ChinaKey Laboratory of Liquor Regulation Technology, State Administration for Market Regulation, Sichuan Institute of Food Inspection, No. 8 Xinwen Road, West Hi-Tech Zone, Chengdu 611731, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, ChinaKey Laboratory of Liquor Regulation Technology, State Administration for Market Regulation, Sichuan Institute of Food Inspection, No. 8 Xinwen Road, West Hi-Tech Zone, Chengdu 611731, ChinaA high-throughput method for the determination of a variety of chemical hazards in poultry muscle and egg samples was established via ultra-performance liquid chromatography–tandem triple quadrupole mass spectrometry (UPLC–QqQ-MS). The sample preparation procedure was developed based on this quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and validated for 280 chemical hazards potentially present in poultry products. The target compounds in poultry samples were extracted with a 1% formic acid–acetonitrile solution (15:85, <i>v</i>/<i>v</i>), and the metal ions in the matrix were chelated by adding ethylenediaminetetraacetic acid disodium salt (Na<sub>2</sub>EDTA). The supernatant was purified using Enhanced Matrix Removal (EMR) lipid sorbent. Chromatographic gradient separation was performed on an ACQUITY UPLC BEH C18 (2.1 mm × 100 mm, 1.7 μm) column with multiple reaction monitoring (MRM) under both negative- and positive-ion mode. Internal standard calibration or matrix-matched calibration was used for the quantitation. The results showed that good linearity was achieved for each target compound with correlation coefficients (R<sup>2</sup>) ≥ 0.99. The limits of detection (LODs) ranged from 0.05 to 10 µg/kg, and the acceptable limits of quantification (LOQs) were determined to be 0.1–20 µg/kg for all 280 compounds. Approximately 90% of the target compounds exhibited mean recoveries ranging from 60% to 120%, with relative standard deviations (RSDs) within 16.2%. This method can be used for the high-throughput rapid detection of prohibited drug residues in poultry eggs due to its easy operation and high accuracy. It was applied in real sample detection, and 43 chemicals including metronidazole were found in 211 poultry samples, with a concentration range of 0.11–638 μg/kg.https://www.mdpi.com/2304-8158/14/10/1660poultry productschemical hazardshigh-throughput detectionUPLC–MS/MS |
| spellingShingle | Rong Chen Lan Chen Mingyue Du Qiaozhen Guo Ciping Zhong Jing Zhang Xiaoqin Yu High-Throughput Determination of Multiclass Chemical Hazards in Poultry Muscles and Eggs Using UPLC–MS/MS Foods poultry products chemical hazards high-throughput detection UPLC–MS/MS |
| title | High-Throughput Determination of Multiclass Chemical Hazards in Poultry Muscles and Eggs Using UPLC–MS/MS |
| title_full | High-Throughput Determination of Multiclass Chemical Hazards in Poultry Muscles and Eggs Using UPLC–MS/MS |
| title_fullStr | High-Throughput Determination of Multiclass Chemical Hazards in Poultry Muscles and Eggs Using UPLC–MS/MS |
| title_full_unstemmed | High-Throughput Determination of Multiclass Chemical Hazards in Poultry Muscles and Eggs Using UPLC–MS/MS |
| title_short | High-Throughput Determination of Multiclass Chemical Hazards in Poultry Muscles and Eggs Using UPLC–MS/MS |
| title_sort | high throughput determination of multiclass chemical hazards in poultry muscles and eggs using uplc ms ms |
| topic | poultry products chemical hazards high-throughput detection UPLC–MS/MS |
| url | https://www.mdpi.com/2304-8158/14/10/1660 |
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