Methotrexate-Induced Accumulation of Fluorescent Annexin V in Collagen-Induced Arthritis
We examined the accumulation of Cy5.5-labeled annexin V in the paws of mice with and without collagen-induced arthritis, with and without methotrexate (MTX) treatment, by near-infrared fluorescence imaging. Fluorescence reflectance imaging (FRI) of paws was performed 48 hr after MTX injection and at...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2005-01-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200504142 |
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| _version_ | 1841564643240181760 |
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| author | Andreas Wunder Eyk Schellenberger Umar Mahmood Alexei Bogdanov Ulf Müller-Ladner Ralph Weissleder Lee Josephson |
| author_facet | Andreas Wunder Eyk Schellenberger Umar Mahmood Alexei Bogdanov Ulf Müller-Ladner Ralph Weissleder Lee Josephson |
| author_sort | Andreas Wunder |
| collection | DOAJ |
| description | We examined the accumulation of Cy5.5-labeled annexin V in the paws of mice with and without collagen-induced arthritis, with and without methotrexate (MTX) treatment, by near-infrared fluorescence imaging. Fluorescence reflectance imaging (FRI) of paws was performed 48 hr after MTX injection and at 10 min and 3 hr after the injection of Cy5.5-annexin V (1 nmol dye per mouse). With arthritic paws, MTX treatment caused a 7-fold increase in fluorescence intensity compared with the paws of untreated mice and a 4-fold increase compared to nonarthritic paws of MTX-treated mice ( p < .001 each). Tissue samples of paws were examined histologically for Cy5.5 fluorescence and by TUNEL staining for apoptosis. Cy5.5-annexin V was seen in the hyperplastic synovia of MTX-treated mice, and TUNEL staining for apoptosis showed apoptotic cells in the hyperplastic synovia. Monitoring the uptake of Cy5.5-annexin V in arthritic paws by FRI provided a method of assessing a response to MTX, a response that was readily quantitated with simple instrumentation and that occurred before conventional measurements of treatment response. |
| format | Article |
| id | doaj-art-ace5406521de439791e927f6aced8747 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-ace5406521de439791e927f6aced87472025-01-02T22:37:02ZengSAGE PublishingMolecular Imaging1536-01212005-01-01410.1162/1535350020050414210.1162_15353500200504142Methotrexate-Induced Accumulation of Fluorescent Annexin V in Collagen-Induced ArthritisAndreas Wunder0Eyk Schellenberger1Umar Mahmood2Alexei Bogdanov3Ulf Müller-Ladner4Ralph Weissleder5Lee JosephsonGerman Cancer Research Center (DKFZ)Charité, Medical Faculty of the Humboldt UniversityHarvard Medical SchoolHarvard Medical SchoolUniversity of RegensburgHarvard Medical SchoolWe examined the accumulation of Cy5.5-labeled annexin V in the paws of mice with and without collagen-induced arthritis, with and without methotrexate (MTX) treatment, by near-infrared fluorescence imaging. Fluorescence reflectance imaging (FRI) of paws was performed 48 hr after MTX injection and at 10 min and 3 hr after the injection of Cy5.5-annexin V (1 nmol dye per mouse). With arthritic paws, MTX treatment caused a 7-fold increase in fluorescence intensity compared with the paws of untreated mice and a 4-fold increase compared to nonarthritic paws of MTX-treated mice ( p < .001 each). Tissue samples of paws were examined histologically for Cy5.5 fluorescence and by TUNEL staining for apoptosis. Cy5.5-annexin V was seen in the hyperplastic synovia of MTX-treated mice, and TUNEL staining for apoptosis showed apoptotic cells in the hyperplastic synovia. Monitoring the uptake of Cy5.5-annexin V in arthritic paws by FRI provided a method of assessing a response to MTX, a response that was readily quantitated with simple instrumentation and that occurred before conventional measurements of treatment response.https://doi.org/10.1162/15353500200504142 |
| spellingShingle | Andreas Wunder Eyk Schellenberger Umar Mahmood Alexei Bogdanov Ulf Müller-Ladner Ralph Weissleder Lee Josephson Methotrexate-Induced Accumulation of Fluorescent Annexin V in Collagen-Induced Arthritis Molecular Imaging |
| title | Methotrexate-Induced Accumulation of Fluorescent Annexin V in Collagen-Induced Arthritis |
| title_full | Methotrexate-Induced Accumulation of Fluorescent Annexin V in Collagen-Induced Arthritis |
| title_fullStr | Methotrexate-Induced Accumulation of Fluorescent Annexin V in Collagen-Induced Arthritis |
| title_full_unstemmed | Methotrexate-Induced Accumulation of Fluorescent Annexin V in Collagen-Induced Arthritis |
| title_short | Methotrexate-Induced Accumulation of Fluorescent Annexin V in Collagen-Induced Arthritis |
| title_sort | methotrexate induced accumulation of fluorescent annexin v in collagen induced arthritis |
| url | https://doi.org/10.1162/15353500200504142 |
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