Maternal Cytomegalovirus Antibodies during Early and Late (Persistent) Phases after Primary Cytomegalovirus Infection during Pregnancy: An Observational Study

Background: We analyzed both early and late (persistent) phases of each cytomegalovirus (CMV) antibody in mothers with primary CMV infection during pregnancy and subsequent congenital CMV infection for a long period from late pregnancy to after delivery using our stored serum samples. Methods: We us...

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Main Authors: Kuniaki Toriyabe, Asa Kitamura, Miki Hagimoto-Akasaka, Toshio Minematsu, Hiroki Takeuchi, Eiji Kondo, Masamichi Kihira, Fumihiro Morikawa, Tomoaki Ikeda
Format: Article
Language:English
Published: IMR Press 2022-12-01
Series:Clinical and Experimental Obstetrics & Gynecology
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Online Access:https://www.imrpress.com/journal/CEOG/49/12/10.31083/j.ceog4912269
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author Kuniaki Toriyabe
Asa Kitamura
Miki Hagimoto-Akasaka
Toshio Minematsu
Hiroki Takeuchi
Eiji Kondo
Masamichi Kihira
Fumihiro Morikawa
Tomoaki Ikeda
author_facet Kuniaki Toriyabe
Asa Kitamura
Miki Hagimoto-Akasaka
Toshio Minematsu
Hiroki Takeuchi
Eiji Kondo
Masamichi Kihira
Fumihiro Morikawa
Tomoaki Ikeda
author_sort Kuniaki Toriyabe
collection DOAJ
description Background: We analyzed both early and late (persistent) phases of each cytomegalovirus (CMV) antibody in mothers with primary CMV infection during pregnancy and subsequent congenital CMV infection for a long period from late pregnancy to after delivery using our stored serum samples. Methods: We used stored serum samples obtained during pregnancy to after delivery from mothers with CMV immunoglobulin (Ig) G seroconversion and subsequent infant congenital CMV infection. CMV antibodies, including CMV IgG titer, IgM titer, and IgG avidity, were assessed using the Denka IgG assay, Denka IgM assay Ver.1 and Ver.2, and Enzygnost IgG assay and Denka IgG avidity assay, respectively. We analyzed the dynamics of each CMV antibody for a long period from late pregnancy to after delivery and correlations of each antibody, calculating Pearson’s correlation coefficients (R2). Results: We used 67 serum samples obtained from 12 included participants between 2013 and 2018. CMV IgG increased until 61 weeks and did not change significantly after. CMV IgM decreased until 52 weeks and did not change significantly after that in both assays. CMV IgG avidity increased until 64 weeks and did not change significantly after that in both assays. In CMV IgM, a strong positive correlation was found (R2 = 0.9326) between the two different IgM assays. Serum results of the late phase (after 60 weeks) were subsumed into the area of high CMV IgG avidity and low CMV IgM titer, which probably was equivalent to the persistent IgM. Conclusions: CMV antibodies in mothers during the late phase of primary infection were in high IgG avidity and low IgM titer, which probably was equivalent to the persistent IgM.
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spelling doaj-art-ace36a5510e1429fadd9b18392c0fa2c2025-08-20T03:49:16ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632022-12-01491226910.31083/j.ceog4912269S0390-6663(22)01943-1Maternal Cytomegalovirus Antibodies during Early and Late (Persistent) Phases after Primary Cytomegalovirus Infection during Pregnancy: An Observational StudyKuniaki Toriyabe0Asa Kitamura1Miki Hagimoto-Akasaka2Toshio Minematsu3Hiroki Takeuchi4Eiji Kondo5Masamichi Kihira6Fumihiro Morikawa7Tomoaki Ikeda8Department of Obstetrics and Gynecology, Mie University Graduate School of Medicine, 514-8507 Tsu, Mie, JapanDepartment of Obstetrics and Gynecology, Mie University Graduate School of Medicine, 514-8507 Tsu, Mie, JapanDepartment of Obstetrics and Gynecology, Mie University Graduate School of Medicine, 514-8507 Tsu, Mie, JapanResearch Center for Disease Control, Aisenkai Nichinan Hospital, 887-0034 Nichinan, Miyazaki, JapanDepartment of Obstetrics and Gynecology, Mie University Graduate School of Medicine, 514-8507 Tsu, Mie, JapanDepartment of Obstetrics and Gynecology, Mie University Graduate School of Medicine, 514-8507 Tsu, Mie, JapanMie Association of Obstetricians and Gynecologists, 514-0003 Tsu, Mie, JapanMie Association of Obstetricians and Gynecologists, 514-0003 Tsu, Mie, JapanDepartment of Obstetrics and Gynecology, Mie University Graduate School of Medicine, 514-8507 Tsu, Mie, JapanBackground: We analyzed both early and late (persistent) phases of each cytomegalovirus (CMV) antibody in mothers with primary CMV infection during pregnancy and subsequent congenital CMV infection for a long period from late pregnancy to after delivery using our stored serum samples. Methods: We used stored serum samples obtained during pregnancy to after delivery from mothers with CMV immunoglobulin (Ig) G seroconversion and subsequent infant congenital CMV infection. CMV antibodies, including CMV IgG titer, IgM titer, and IgG avidity, were assessed using the Denka IgG assay, Denka IgM assay Ver.1 and Ver.2, and Enzygnost IgG assay and Denka IgG avidity assay, respectively. We analyzed the dynamics of each CMV antibody for a long period from late pregnancy to after delivery and correlations of each antibody, calculating Pearson’s correlation coefficients (R2). Results: We used 67 serum samples obtained from 12 included participants between 2013 and 2018. CMV IgG increased until 61 weeks and did not change significantly after. CMV IgM decreased until 52 weeks and did not change significantly after that in both assays. CMV IgG avidity increased until 64 weeks and did not change significantly after that in both assays. In CMV IgM, a strong positive correlation was found (R2 = 0.9326) between the two different IgM assays. Serum results of the late phase (after 60 weeks) were subsumed into the area of high CMV IgG avidity and low CMV IgM titer, which probably was equivalent to the persistent IgM. Conclusions: CMV antibodies in mothers during the late phase of primary infection were in high IgG avidity and low IgM titer, which probably was equivalent to the persistent IgM.https://www.imrpress.com/journal/CEOG/49/12/10.31083/j.ceog4912269cytomegaloviruspregnancyserumimmunoglobulin
spellingShingle Kuniaki Toriyabe
Asa Kitamura
Miki Hagimoto-Akasaka
Toshio Minematsu
Hiroki Takeuchi
Eiji Kondo
Masamichi Kihira
Fumihiro Morikawa
Tomoaki Ikeda
Maternal Cytomegalovirus Antibodies during Early and Late (Persistent) Phases after Primary Cytomegalovirus Infection during Pregnancy: An Observational Study
Clinical and Experimental Obstetrics & Gynecology
cytomegalovirus
pregnancy
serum
immunoglobulin
title Maternal Cytomegalovirus Antibodies during Early and Late (Persistent) Phases after Primary Cytomegalovirus Infection during Pregnancy: An Observational Study
title_full Maternal Cytomegalovirus Antibodies during Early and Late (Persistent) Phases after Primary Cytomegalovirus Infection during Pregnancy: An Observational Study
title_fullStr Maternal Cytomegalovirus Antibodies during Early and Late (Persistent) Phases after Primary Cytomegalovirus Infection during Pregnancy: An Observational Study
title_full_unstemmed Maternal Cytomegalovirus Antibodies during Early and Late (Persistent) Phases after Primary Cytomegalovirus Infection during Pregnancy: An Observational Study
title_short Maternal Cytomegalovirus Antibodies during Early and Late (Persistent) Phases after Primary Cytomegalovirus Infection during Pregnancy: An Observational Study
title_sort maternal cytomegalovirus antibodies during early and late persistent phases after primary cytomegalovirus infection during pregnancy an observational study
topic cytomegalovirus
pregnancy
serum
immunoglobulin
url https://www.imrpress.com/journal/CEOG/49/12/10.31083/j.ceog4912269
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