Magnetic Nanoparticles Immobilization and Functionalization for Biosensor Applications
We describe an approach for E. coli bacteria detection using an electrochemical immunosensor. The immunosensor was based on functionalized magnetic nanoparticles immobilized onto bare gold electrode. Cyclic voltammetry and impedance spectroscopy was performed before and after magnetic nanoparticles...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Electrochemistry |
| Online Access: | http://dx.doi.org/10.4061/2011/421387 |
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| _version_ | 1832560335844802560 |
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| author | M. B. Mejri A. Tlili A. Abdelghani |
| author_facet | M. B. Mejri A. Tlili A. Abdelghani |
| author_sort | M. B. Mejri |
| collection | DOAJ |
| description | We describe an approach for E. coli bacteria detection using an electrochemical immunosensor. The immunosensor was based on functionalized magnetic nanoparticles immobilized onto bare gold electrode. Cyclic voltammetry and impedance spectroscopy was performed before and after magnetic nanoparticles deposition. The magnetic nanoparticles functionalized with anti-E. coli polyclonal antibody were used for bacteria detection. Lytic T4-phage was used to confirm the success recognition of bacteria with the developed immunosensor. The specificity of the immunosensor was tested against Enterococcus faecium bacteria. A limit detection of 103 CFU/mL E. coli bacteria was obtained with a good reproducibility. |
| format | Article |
| id | doaj-art-acd63dae713a44aa958b22b392df925f |
| institution | Kabale University |
| issn | 2090-3537 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Electrochemistry |
| spelling | doaj-art-acd63dae713a44aa958b22b392df925f2025-02-03T01:27:51ZengWileyInternational Journal of Electrochemistry2090-35372011-01-01201110.4061/2011/421387421387Magnetic Nanoparticles Immobilization and Functionalization for Biosensor ApplicationsM. B. Mejri0A. Tlili1A. Abdelghani2Nanotechnology Laboratory, Centre Urbain Nord, University of Carthage, INSAT, BP 676, Charguia Cedex 1080, TunisiaNanotechnology Laboratory, Centre Urbain Nord, University of Carthage, INSAT, BP 676, Charguia Cedex 1080, TunisiaNanotechnology Laboratory, Centre Urbain Nord, University of Carthage, INSAT, BP 676, Charguia Cedex 1080, TunisiaWe describe an approach for E. coli bacteria detection using an electrochemical immunosensor. The immunosensor was based on functionalized magnetic nanoparticles immobilized onto bare gold electrode. Cyclic voltammetry and impedance spectroscopy was performed before and after magnetic nanoparticles deposition. The magnetic nanoparticles functionalized with anti-E. coli polyclonal antibody were used for bacteria detection. Lytic T4-phage was used to confirm the success recognition of bacteria with the developed immunosensor. The specificity of the immunosensor was tested against Enterococcus faecium bacteria. A limit detection of 103 CFU/mL E. coli bacteria was obtained with a good reproducibility.http://dx.doi.org/10.4061/2011/421387 |
| spellingShingle | M. B. Mejri A. Tlili A. Abdelghani Magnetic Nanoparticles Immobilization and Functionalization for Biosensor Applications International Journal of Electrochemistry |
| title | Magnetic Nanoparticles Immobilization and Functionalization for Biosensor Applications |
| title_full | Magnetic Nanoparticles Immobilization and Functionalization for Biosensor Applications |
| title_fullStr | Magnetic Nanoparticles Immobilization and Functionalization for Biosensor Applications |
| title_full_unstemmed | Magnetic Nanoparticles Immobilization and Functionalization for Biosensor Applications |
| title_short | Magnetic Nanoparticles Immobilization and Functionalization for Biosensor Applications |
| title_sort | magnetic nanoparticles immobilization and functionalization for biosensor applications |
| url | http://dx.doi.org/10.4061/2011/421387 |
| work_keys_str_mv | AT mbmejri magneticnanoparticlesimmobilizationandfunctionalizationforbiosensorapplications AT atlili magneticnanoparticlesimmobilizationandfunctionalizationforbiosensorapplications AT aabdelghani magneticnanoparticlesimmobilizationandfunctionalizationforbiosensorapplications |