Differential efficacy of olfactory neurospheres from deviated nasal septum and chronic rhinosinusitis patients in regenerating olfactory epithelium
Abstract Background Olfactory epithelial stem cells hold significant potential for treating olfactory dysfunction by facilitating tissue maintenance and repair. Understanding the inherent qualities of these stem cells is crucial for optimizing their therapeutic efficacy. Methods Olfactory epithelial...
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2025-04-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13287-025-04270-0 |
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| author | Rong-San Jiang Chiang-Wen Lee Yu-Hsuan Lin Jing-Jie Wang Jia-Bin Liao Kuo-Ti Peng Yao-Chang chiang Pei-Ling Chi |
| author_facet | Rong-San Jiang Chiang-Wen Lee Yu-Hsuan Lin Jing-Jie Wang Jia-Bin Liao Kuo-Ti Peng Yao-Chang chiang Pei-Ling Chi |
| author_sort | Rong-San Jiang |
| collection | DOAJ |
| description | Abstract Background Olfactory epithelial stem cells hold significant potential for treating olfactory dysfunction by facilitating tissue maintenance and repair. Understanding the inherent qualities of these stem cells is crucial for optimizing their therapeutic efficacy. Methods Olfactory epithelial samples were collected from patients with deviated nasal septum (DNS) and chronic rhinosinusitis (CRS). These were cultured to form olfactory neurospheres (ONS), which were then analyzed for neural stem cell markers, neurotrophic factor production, and their ability to differentiate into olfactory sensory neurons (OSNs). The regenerative efficacy of these ONS was tested in a methimazole-induced hyposmic mouse model, with the effects on cellular senescence, apoptosis, and proliferation in the olfactory epithelium assessed. Results Both DNS- and CRS-derived ONS exhibited neural stem cell characteristics. DNS-ONS displayed superior self-renewal capacity and higher neurotrophic factor production compared to CRS-ONS, which showed impaired OSN maturation and lower neurotrophic factor levels. In vivo, DNS-ONS were more effective in restoring olfaction, as evidenced by reduced cellular senescence, decreased apoptosis, and increased cell proliferation in the OE of methimazole-induced hyposmic mice. Conclusions These findings highlight the importance of selecting the appropriate ONS source for therapeutic applications, with DNS-ONS showing greater promise for olfactory epithelium repair and olfactory function restoration. |
| format | Article |
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| institution | OA Journals |
| issn | 1757-6512 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
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| series | Stem Cell Research & Therapy |
| spelling | doaj-art-acd4a643400c4882b60cd2f10d0967f32025-08-20T01:53:19ZengBMCStem Cell Research & Therapy1757-65122025-04-0116112110.1186/s13287-025-04270-0Differential efficacy of olfactory neurospheres from deviated nasal septum and chronic rhinosinusitis patients in regenerating olfactory epitheliumRong-San Jiang0Chiang-Wen Lee1Yu-Hsuan Lin2Jing-Jie Wang3Jia-Bin Liao4Kuo-Ti Peng5Yao-Chang chiang6Pei-Ling Chi7Department of Otolaryngology, Tungs’ Taichung MetroHarbor HospitalDepartment of Nursing, Division of Basic Medical Sciences, and Chronic Diseases and Health Promotion Research Center, Chang Gung University of Science and TechnologyDepartment of Otolaryngology, Head and Neck Surgery, Kaohsiung Veterans General HospitalDepartment of Otolaryngology, Taichung Veterans General HospitalDepartment of Pathology and Laboratory Medicine, Kaohsiung Veterans General HospitalDepartment of Orthopaedic Surgery, Chang Gung Memorial HospitalDepartment of Nursing, Division of Basic Medical Sciences, and Chronic Diseases and Health Promotion Research Center, Chang Gung University of Science and TechnologyDepartment of Medical Education and Research, Kaohsiung Veterans General HospitalAbstract Background Olfactory epithelial stem cells hold significant potential for treating olfactory dysfunction by facilitating tissue maintenance and repair. Understanding the inherent qualities of these stem cells is crucial for optimizing their therapeutic efficacy. Methods Olfactory epithelial samples were collected from patients with deviated nasal septum (DNS) and chronic rhinosinusitis (CRS). These were cultured to form olfactory neurospheres (ONS), which were then analyzed for neural stem cell markers, neurotrophic factor production, and their ability to differentiate into olfactory sensory neurons (OSNs). The regenerative efficacy of these ONS was tested in a methimazole-induced hyposmic mouse model, with the effects on cellular senescence, apoptosis, and proliferation in the olfactory epithelium assessed. Results Both DNS- and CRS-derived ONS exhibited neural stem cell characteristics. DNS-ONS displayed superior self-renewal capacity and higher neurotrophic factor production compared to CRS-ONS, which showed impaired OSN maturation and lower neurotrophic factor levels. In vivo, DNS-ONS were more effective in restoring olfaction, as evidenced by reduced cellular senescence, decreased apoptosis, and increased cell proliferation in the OE of methimazole-induced hyposmic mice. Conclusions These findings highlight the importance of selecting the appropriate ONS source for therapeutic applications, with DNS-ONS showing greater promise for olfactory epithelium repair and olfactory function restoration.https://doi.org/10.1186/s13287-025-04270-0Olfactory neurospheresRegenerative medicineOlfactory dysfunction |
| spellingShingle | Rong-San Jiang Chiang-Wen Lee Yu-Hsuan Lin Jing-Jie Wang Jia-Bin Liao Kuo-Ti Peng Yao-Chang chiang Pei-Ling Chi Differential efficacy of olfactory neurospheres from deviated nasal septum and chronic rhinosinusitis patients in regenerating olfactory epithelium Stem Cell Research & Therapy Olfactory neurospheres Regenerative medicine Olfactory dysfunction |
| title | Differential efficacy of olfactory neurospheres from deviated nasal septum and chronic rhinosinusitis patients in regenerating olfactory epithelium |
| title_full | Differential efficacy of olfactory neurospheres from deviated nasal septum and chronic rhinosinusitis patients in regenerating olfactory epithelium |
| title_fullStr | Differential efficacy of olfactory neurospheres from deviated nasal septum and chronic rhinosinusitis patients in regenerating olfactory epithelium |
| title_full_unstemmed | Differential efficacy of olfactory neurospheres from deviated nasal septum and chronic rhinosinusitis patients in regenerating olfactory epithelium |
| title_short | Differential efficacy of olfactory neurospheres from deviated nasal septum and chronic rhinosinusitis patients in regenerating olfactory epithelium |
| title_sort | differential efficacy of olfactory neurospheres from deviated nasal septum and chronic rhinosinusitis patients in regenerating olfactory epithelium |
| topic | Olfactory neurospheres Regenerative medicine Olfactory dysfunction |
| url | https://doi.org/10.1186/s13287-025-04270-0 |
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