Quantitative analysis and pharmacological assessment of the methanolic and silver nanoparticles of Chamaecostus cuspidatus
Nature has various medicinal properties worldwide, and natural environmental sources are the source of many alternative contemporary medications. Many civilizations employ plants as medicine, and because of their bioactive chemicals, they may produce potent pharmaceuticals. Synthesis of Silver nanop...
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Elsevier
2025-07-01
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| author | Bibi Hafsa Azra Sumera Nazneen Sri Sudewi Fransiska Sri Herwahyu Krismastuti Apurba Das Muhammad Zulfajiri Akhtar Rasool |
| author_facet | Bibi Hafsa Azra Sumera Nazneen Sri Sudewi Fransiska Sri Herwahyu Krismastuti Apurba Das Muhammad Zulfajiri Akhtar Rasool |
| author_sort | Bibi Hafsa Azra |
| collection | DOAJ |
| description | Nature has various medicinal properties worldwide, and natural environmental sources are the source of many alternative contemporary medications. Many civilizations employ plants as medicine, and because of their bioactive chemicals, they may produce potent pharmaceuticals. Synthesis of Silver nanoparticles of these valuable plants possesses great potential in the modern therapeutic sector. Chamaecostus cuspidatus, commonly known as the Insulin plant due to its abundant secondary metabolites, provides a hypoglycemic effect and has considerable antioxidant and anticancer properties. The total alkaloid, tannins, and phenolic contents in methanolic and aqueous AgNP extracts of C. cuspidatus were quantified using a spectrophotometric approach. The DPPH assay is a quick and straightforward approach to assessing putative antioxidants, with ascorbic acid as the standard. In ascorbic acid, %inhibition is 50.8 at 10 μg/ml, the highest with the lowest IC50 value, i.e., −3.3. Compared to ascorbic acid, the percentage inhibition of methanolic and AgNP extract at 10 μg/ml is 12.57 and −0.57, respectively, and IC50 was calculated as 0.52 and −0.03. The MTT assay was carried out to detect anticancer activity. The IC50 value was calculated using a linear regression equation, and it was found to be 123.32 μg/ml in methanolic extract and 101.66 μg/ml in AgNP extract. Extracts with IC50 values between 21 and 200 μg/mL were considered moderately cytotoxic by the National Cancer Institute and the Geran protocol. |
| format | Article |
| id | doaj-art-acd048e9960c422b941a0c7526b98995 |
| institution | Kabale University |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
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| series | Results in Chemistry |
| spelling | doaj-art-acd048e9960c422b941a0c7526b989952025-08-20T03:39:09ZengElsevierResults in Chemistry2211-71562025-07-011610232510.1016/j.rechem.2025.102325Quantitative analysis and pharmacological assessment of the methanolic and silver nanoparticles of Chamaecostus cuspidatusBibi Hafsa Azra0Sumera Nazneen1Sri Sudewi2Fransiska Sri Herwahyu Krismastuti3Apurba Das4Muhammad Zulfajiri5Akhtar Rasool6Department of Botany, Anwarul Uloom College, Osmania University, Hyderabad, Telangana State, IndiaDepartment of Botany, Anwarul Uloom College, Osmania University, Hyderabad, Telangana State, IndiaDepartment of Pharmacy, Faculty of Mathematics and Natural Science, Universitas Sam Ratulangi, Manado, IndonesiaResearch Center for Chemistry - National Research and Innovation Agency (BRIN), KST BJ Habibie, Building 452, Setu, Tangerang, Selatan, 15314, Indonesia; Corresponding author.Department of Plant Pathology, Assam Agricultural University, Jorhat 785013, IndiaDepartment of Chemistry Education, Universitas Serambi Mekkah, Banda Aceh, IndonesiaResearch Center for Chemistry - National Research and Innovation Agency (BRIN), KST BJ Habibie, Building 452, Setu, Tangerang, Selatan, 15314, Indonesia; Department of Biotechnology, Manav Rachna International Institute of Research and Studies, Surajkund, Haryana 121004, India; Corresponding author at: Research Center for Chemistry - National Research and Innovation Agency (BRIN), KST BJ Habibie, Building 452, Setu, Tangerang, Selatan, 15314, Indonesia.Nature has various medicinal properties worldwide, and natural environmental sources are the source of many alternative contemporary medications. Many civilizations employ plants as medicine, and because of their bioactive chemicals, they may produce potent pharmaceuticals. Synthesis of Silver nanoparticles of these valuable plants possesses great potential in the modern therapeutic sector. Chamaecostus cuspidatus, commonly known as the Insulin plant due to its abundant secondary metabolites, provides a hypoglycemic effect and has considerable antioxidant and anticancer properties. The total alkaloid, tannins, and phenolic contents in methanolic and aqueous AgNP extracts of C. cuspidatus were quantified using a spectrophotometric approach. The DPPH assay is a quick and straightforward approach to assessing putative antioxidants, with ascorbic acid as the standard. In ascorbic acid, %inhibition is 50.8 at 10 μg/ml, the highest with the lowest IC50 value, i.e., −3.3. Compared to ascorbic acid, the percentage inhibition of methanolic and AgNP extract at 10 μg/ml is 12.57 and −0.57, respectively, and IC50 was calculated as 0.52 and −0.03. The MTT assay was carried out to detect anticancer activity. The IC50 value was calculated using a linear regression equation, and it was found to be 123.32 μg/ml in methanolic extract and 101.66 μg/ml in AgNP extract. Extracts with IC50 values between 21 and 200 μg/mL were considered moderately cytotoxic by the National Cancer Institute and the Geran protocol.http://www.sciencedirect.com/science/article/pii/S221171562500308XChamaecostus cuspidatusDPPHMTT assayAnticancer activityAntioxidant activity |
| spellingShingle | Bibi Hafsa Azra Sumera Nazneen Sri Sudewi Fransiska Sri Herwahyu Krismastuti Apurba Das Muhammad Zulfajiri Akhtar Rasool Quantitative analysis and pharmacological assessment of the methanolic and silver nanoparticles of Chamaecostus cuspidatus Results in Chemistry Chamaecostus cuspidatus DPPH MTT assay Anticancer activity Antioxidant activity |
| title | Quantitative analysis and pharmacological assessment of the methanolic and silver nanoparticles of Chamaecostus cuspidatus |
| title_full | Quantitative analysis and pharmacological assessment of the methanolic and silver nanoparticles of Chamaecostus cuspidatus |
| title_fullStr | Quantitative analysis and pharmacological assessment of the methanolic and silver nanoparticles of Chamaecostus cuspidatus |
| title_full_unstemmed | Quantitative analysis and pharmacological assessment of the methanolic and silver nanoparticles of Chamaecostus cuspidatus |
| title_short | Quantitative analysis and pharmacological assessment of the methanolic and silver nanoparticles of Chamaecostus cuspidatus |
| title_sort | quantitative analysis and pharmacological assessment of the methanolic and silver nanoparticles of chamaecostus cuspidatus |
| topic | Chamaecostus cuspidatus DPPH MTT assay Anticancer activity Antioxidant activity |
| url | http://www.sciencedirect.com/science/article/pii/S221171562500308X |
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