Green-based anti-biofilm nanoformulations against bacterial contaminations in nosocomial environments
α-amylase enzyme molecules were conjugated to biocompatible and biodegradable ad-hoc synthetized polycaprolactone (PCL) nanoparticles, in order to fabricate an effective and innovative nanoplatform for the treatment of nosocomial Staphylococcus aureus contaminations. Indeed, PCL was chosen as the po...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025013155 |
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| author | Giovanni Lo Bello Elena Dellacasa Giacomo Damonte Debora Caviglia Anna Maria Schito Roberto Raiteri Orietta Monticelli Marina Sartini Maria Luisa Cristina Laura Pastorino |
| author_facet | Giovanni Lo Bello Elena Dellacasa Giacomo Damonte Debora Caviglia Anna Maria Schito Roberto Raiteri Orietta Monticelli Marina Sartini Maria Luisa Cristina Laura Pastorino |
| author_sort | Giovanni Lo Bello |
| collection | DOAJ |
| description | α-amylase enzyme molecules were conjugated to biocompatible and biodegradable ad-hoc synthetized polycaprolactone (PCL) nanoparticles, in order to fabricate an effective and innovative nanoplatform for the treatment of nosocomial Staphylococcus aureus contaminations. Indeed, PCL was chosen as the polymer matrix due to its features and the easy scalability of its synthesis, which enables to obtain a linear or star-shaped architecture with high functionality as in the polymer used.The developed nanoformulation underwent extensive structural and functional characterization to evaluate nanoparticles size and morphology before and after enzyme immobilization. Its antibiofilm effectiveness was then validated against bacterial strains isolated from hospital surfaces, demonstrating its potential for practical anti-biofilm applications. The obtained results demonstrated that the system prepared from the enzyme-conjugated nanoparticles exhibited a significant enzymatic activity and an efficient ability to degrade the protective bacterial biofilm. The proposed nanoformulation can therefore be considered an effective and completely environmentally friendly material for surface disinfection in healthcare facilities, which can be safely used in different environment (e.g. sinks and pipes) or medical equipment surfaces (e.g. touch screens). |
| format | Article |
| id | doaj-art-accc7166840c47cd9775d8965630ca65 |
| institution | DOAJ |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-accc7166840c47cd9775d8965630ca652025-08-20T02:45:33ZengElsevierHeliyon2405-84402025-02-01114e4293410.1016/j.heliyon.2025.e42934Green-based anti-biofilm nanoformulations against bacterial contaminations in nosocomial environmentsGiovanni Lo Bello0Elena Dellacasa1Giacomo Damonte2Debora Caviglia3Anna Maria Schito4Roberto Raiteri5Orietta Monticelli6Marina Sartini7Maria Luisa Cristina8Laura Pastorino9Department of Informatics, Bioengineering, Robotics, and System Engineering, University of Genova, Via Opera Pia 13, 16145, Genova, ItalyDepartment of Informatics, Bioengineering, Robotics, and System Engineering, University of Genova, Via Opera Pia 13, 16145, Genova, ItalyDepartment of Chemistry and Industrial Chemistry, University of Genova, Via Dodecaneso 31, 16146, Genova, ItalyDepartment of Integrated Surgical and Diagnostical Sciences, University of Genoa, Viale Benedetto XV 6, 16132, Genoa, ItalyDepartment of Integrated Surgical and Diagnostical Sciences, University of Genoa, Viale Benedetto XV 6, 16132, Genoa, ItalyDepartment of Informatics, Bioengineering, Robotics, and System Engineering, University of Genova, Via Opera Pia 13, 16145, Genova, ItalyDepartment of Chemistry and Industrial Chemistry, University of Genova, Via Dodecaneso 31, 16146, Genova, ItalyDepartment of Health Science, University of Genova, Via Pastore 1, 16132, Genova, Italy; E.O. Ospedali Galliera, Hygiene Unit, Via Mura delle Cappuccine 14, 16128, Genova, ItalyDepartment of Health Science, University of Genova, Via Pastore 1, 16132, Genova, Italy; E.O. Ospedali Galliera, Hygiene Unit, Via Mura delle Cappuccine 14, 16128, Genova, ItalyDepartment of Informatics, Bioengineering, Robotics, and System Engineering, University of Genova, Via Opera Pia 13, 16145, Genova, Italy; Corresponding author.α-amylase enzyme molecules were conjugated to biocompatible and biodegradable ad-hoc synthetized polycaprolactone (PCL) nanoparticles, in order to fabricate an effective and innovative nanoplatform for the treatment of nosocomial Staphylococcus aureus contaminations. Indeed, PCL was chosen as the polymer matrix due to its features and the easy scalability of its synthesis, which enables to obtain a linear or star-shaped architecture with high functionality as in the polymer used.The developed nanoformulation underwent extensive structural and functional characterization to evaluate nanoparticles size and morphology before and after enzyme immobilization. Its antibiofilm effectiveness was then validated against bacterial strains isolated from hospital surfaces, demonstrating its potential for practical anti-biofilm applications. The obtained results demonstrated that the system prepared from the enzyme-conjugated nanoparticles exhibited a significant enzymatic activity and an efficient ability to degrade the protective bacterial biofilm. The proposed nanoformulation can therefore be considered an effective and completely environmentally friendly material for surface disinfection in healthcare facilities, which can be safely used in different environment (e.g. sinks and pipes) or medical equipment surfaces (e.g. touch screens).http://www.sciencedirect.com/science/article/pii/S2405844025013155Nosocomial infectionsStaphylococcus aureusα-amylasePCL nanoparticlesAntibiofilm nanoplatform |
| spellingShingle | Giovanni Lo Bello Elena Dellacasa Giacomo Damonte Debora Caviglia Anna Maria Schito Roberto Raiteri Orietta Monticelli Marina Sartini Maria Luisa Cristina Laura Pastorino Green-based anti-biofilm nanoformulations against bacterial contaminations in nosocomial environments Heliyon Nosocomial infections Staphylococcus aureus α-amylase PCL nanoparticles Antibiofilm nanoplatform |
| title | Green-based anti-biofilm nanoformulations against bacterial contaminations in nosocomial environments |
| title_full | Green-based anti-biofilm nanoformulations against bacterial contaminations in nosocomial environments |
| title_fullStr | Green-based anti-biofilm nanoformulations against bacterial contaminations in nosocomial environments |
| title_full_unstemmed | Green-based anti-biofilm nanoformulations against bacterial contaminations in nosocomial environments |
| title_short | Green-based anti-biofilm nanoformulations against bacterial contaminations in nosocomial environments |
| title_sort | green based anti biofilm nanoformulations against bacterial contaminations in nosocomial environments |
| topic | Nosocomial infections Staphylococcus aureus α-amylase PCL nanoparticles Antibiofilm nanoplatform |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025013155 |
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