Temporal multiomics gene expression data of human embryonic stem cell-derived cardiomyocyte differentiation
Abstract Human embryonic stem cells (hESCs) serve as a valuable in vitro model for studying early human developmental processes due to their ability to differentiate into all three germ layers. Here, we present a comprehensive multi-omics dataset generated by differentiating hESCs into cardiomyocyte...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
|
| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-05655-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849344143985213440 |
|---|---|
| author | Abdurrahman Keskin Hani J. Shayya Dario Sirabella Achchhe Patel Barbara Corneo Marko Jovanovic |
| author_facet | Abdurrahman Keskin Hani J. Shayya Dario Sirabella Achchhe Patel Barbara Corneo Marko Jovanovic |
| author_sort | Abdurrahman Keskin |
| collection | DOAJ |
| description | Abstract Human embryonic stem cells (hESCs) serve as a valuable in vitro model for studying early human developmental processes due to their ability to differentiate into all three germ layers. Here, we present a comprehensive multi-omics dataset generated by differentiating hESCs into cardiomyocytes via the mesodermal lineage, collecting samples at 10 distinct time points. We measured mRNA levels by mRNA sequencing (mRNA-seq), translation levels by ribosome profiling (Ribo-seq), and protein levels by quantitative mass spectrometry-based proteomics. Technical validation confirmed high quality and reproducibility across all datasets, with strong correlations between replicates. This extensive dataset provides critical insights into the complex regulatory mechanisms of cardiomyocyte differentiation and serves as a valuable resource for the research community, aiding in the exploration of mammalian development and gene regulation. |
| format | Article |
| id | doaj-art-accc5d614df945dbb3dfc91b68f9cdea |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-accc5d614df945dbb3dfc91b68f9cdea2025-08-20T03:42:44ZengNature PortfolioScientific Data2052-44632025-07-0112111310.1038/s41597-025-05655-9Temporal multiomics gene expression data of human embryonic stem cell-derived cardiomyocyte differentiationAbdurrahman Keskin0Hani J. Shayya1Dario Sirabella2Achchhe Patel3Barbara Corneo4Marko Jovanovic5Department of Biological Sciences, Columbia UniversityMortimer B. Zuckerman Mind, Brain and Behavior Institute, Columbia UniversityColumbia Stem Cell Initiative, Stem Cell Core, Columbia University Irving Medical CenterColumbia Stem Cell Initiative, Stem Cell Core, Columbia University Irving Medical CenterColumbia Stem Cell Initiative, Stem Cell Core, Columbia University Irving Medical CenterDepartment of Biological Sciences, Columbia UniversityAbstract Human embryonic stem cells (hESCs) serve as a valuable in vitro model for studying early human developmental processes due to their ability to differentiate into all three germ layers. Here, we present a comprehensive multi-omics dataset generated by differentiating hESCs into cardiomyocytes via the mesodermal lineage, collecting samples at 10 distinct time points. We measured mRNA levels by mRNA sequencing (mRNA-seq), translation levels by ribosome profiling (Ribo-seq), and protein levels by quantitative mass spectrometry-based proteomics. Technical validation confirmed high quality and reproducibility across all datasets, with strong correlations between replicates. This extensive dataset provides critical insights into the complex regulatory mechanisms of cardiomyocyte differentiation and serves as a valuable resource for the research community, aiding in the exploration of mammalian development and gene regulation.https://doi.org/10.1038/s41597-025-05655-9 |
| spellingShingle | Abdurrahman Keskin Hani J. Shayya Dario Sirabella Achchhe Patel Barbara Corneo Marko Jovanovic Temporal multiomics gene expression data of human embryonic stem cell-derived cardiomyocyte differentiation Scientific Data |
| title | Temporal multiomics gene expression data of human embryonic stem cell-derived cardiomyocyte differentiation |
| title_full | Temporal multiomics gene expression data of human embryonic stem cell-derived cardiomyocyte differentiation |
| title_fullStr | Temporal multiomics gene expression data of human embryonic stem cell-derived cardiomyocyte differentiation |
| title_full_unstemmed | Temporal multiomics gene expression data of human embryonic stem cell-derived cardiomyocyte differentiation |
| title_short | Temporal multiomics gene expression data of human embryonic stem cell-derived cardiomyocyte differentiation |
| title_sort | temporal multiomics gene expression data of human embryonic stem cell derived cardiomyocyte differentiation |
| url | https://doi.org/10.1038/s41597-025-05655-9 |
| work_keys_str_mv | AT abdurrahmankeskin temporalmultiomicsgeneexpressiondataofhumanembryonicstemcellderivedcardiomyocytedifferentiation AT hanijshayya temporalmultiomicsgeneexpressiondataofhumanembryonicstemcellderivedcardiomyocytedifferentiation AT dariosirabella temporalmultiomicsgeneexpressiondataofhumanembryonicstemcellderivedcardiomyocytedifferentiation AT achchhepatel temporalmultiomicsgeneexpressiondataofhumanembryonicstemcellderivedcardiomyocytedifferentiation AT barbaracorneo temporalmultiomicsgeneexpressiondataofhumanembryonicstemcellderivedcardiomyocytedifferentiation AT markojovanovic temporalmultiomicsgeneexpressiondataofhumanembryonicstemcellderivedcardiomyocytedifferentiation |