Genome-wide identification and expression analysis of sucrose phosphate synthase and sucrose-6-phosphate phosphatase family genes in Camellia sinensis
Sucrose phosphate synthetase (SPS, EC 2.4.1.14) and sucrose phosphate phosphatase (SPP, EC 3.1.3.24) are two key enzymes for sucrose biosynthesis, which play essential roles in growth, development and stress responses of plants. However, the roles of SPS and SPP have not been illustrated well in tea...
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| Language: | English |
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Maximum Academic Press
2024-01-01
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| Series: | Beverage Plant Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/bpr-0024-0007 |
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| author | Shicai Liang Huan Wang Hiroto Yamashita Shuning Zhang Xuxu Lang Jiaxuan Yue Shan He Yu Wang Kai Fan Zhaotang Ding Takashi Ikka Wenjun Qian |
| author_facet | Shicai Liang Huan Wang Hiroto Yamashita Shuning Zhang Xuxu Lang Jiaxuan Yue Shan He Yu Wang Kai Fan Zhaotang Ding Takashi Ikka Wenjun Qian |
| author_sort | Shicai Liang |
| collection | DOAJ |
| description | Sucrose phosphate synthetase (SPS, EC 2.4.1.14) and sucrose phosphate phosphatase (SPP, EC 3.1.3.24) are two key enzymes for sucrose biosynthesis, which play essential roles in growth, development and stress responses of plants. However, the roles of SPS and SPP have not been illustrated well in tea plants until now. In this study, a unique CsSPP and five CsSPSs (CsSPS1-5) were identified from the tea plant genome. Bioinformatic analysis results found that CsSPP and CsSPSs were clustered together with the known SPPs and SPSs of other species, respectively, and their amino acid sequences contain the conserved domains required for sucrose biosynthesis. Tissue-specific analysis results showed that CsSPP and CsSPSs were widely involved in vegetative and reproductive growth of tea plant, among which the transcription levels of CsSPP was highest in immature stem, while CsSPSs were highest in flower. Spatio-temporal expression analysis results showed that all of these genes are involved in abiotic stress responses of tea plants. Meanwhile, SPS activity and the contents of sucrose, glucose, fructose, and total soluble sugar in 'Shuchazao' cultivar increased more than that of 'Baiye1' under low temperature conditions. Correlation analysis results showed that the expression profiles of CsSPS2/4/5 were significantly and positively correlated with sucrose content in 'Shuchazao' cultivars under low temperature conditions, suggesting the significant roles of these genes in sucrose accumulation. In conclusion, this study will provide a theoretical basis for further functional research of SPS and SPP in plants. |
| format | Article |
| id | doaj-art-acc41582e8cb4066a68cd23c83626e2b |
| institution | OA Journals |
| issn | 2769-2108 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Beverage Plant Research |
| spelling | doaj-art-acc41582e8cb4066a68cd23c83626e2b2025-08-20T02:27:12ZengMaximum Academic PressBeverage Plant Research2769-21082024-01-014111510.48130/bpr-0024-0007bpr-0024-0007Genome-wide identification and expression analysis of sucrose phosphate synthase and sucrose-6-phosphate phosphatase family genes in Camellia sinensisShicai Liang0Huan Wang1Hiroto Yamashita2Shuning Zhang3Xuxu Lang4Jiaxuan Yue5Shan He6Yu Wang7Kai Fan8Zhaotang Ding9Takashi Ikka10Wenjun Qian11College of Horticulture, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao 266109, ChinaFaculty of Agriculture, Shizuoka University, Shizuoka 4228529, JapanFaculty of Agriculture, Shizuoka University, Shizuoka 4228529, JapanCollege of Horticulture, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao 266109, ChinaTea Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaFaculty of Agriculture, Shizuoka University, Shizuoka 4228529, JapanCollege of Horticulture, Qingdao Agricultural University, Qingdao 266109, ChinaSucrose phosphate synthetase (SPS, EC 2.4.1.14) and sucrose phosphate phosphatase (SPP, EC 3.1.3.24) are two key enzymes for sucrose biosynthesis, which play essential roles in growth, development and stress responses of plants. However, the roles of SPS and SPP have not been illustrated well in tea plants until now. In this study, a unique CsSPP and five CsSPSs (CsSPS1-5) were identified from the tea plant genome. Bioinformatic analysis results found that CsSPP and CsSPSs were clustered together with the known SPPs and SPSs of other species, respectively, and their amino acid sequences contain the conserved domains required for sucrose biosynthesis. Tissue-specific analysis results showed that CsSPP and CsSPSs were widely involved in vegetative and reproductive growth of tea plant, among which the transcription levels of CsSPP was highest in immature stem, while CsSPSs were highest in flower. Spatio-temporal expression analysis results showed that all of these genes are involved in abiotic stress responses of tea plants. Meanwhile, SPS activity and the contents of sucrose, glucose, fructose, and total soluble sugar in 'Shuchazao' cultivar increased more than that of 'Baiye1' under low temperature conditions. Correlation analysis results showed that the expression profiles of CsSPS2/4/5 were significantly and positively correlated with sucrose content in 'Shuchazao' cultivars under low temperature conditions, suggesting the significant roles of these genes in sucrose accumulation. In conclusion, this study will provide a theoretical basis for further functional research of SPS and SPP in plants.https://www.maxapress.com/article/doi/10.48130/bpr-0024-0007camellia sinensissppspstissue-specific analysisspatio-temporal analysis |
| spellingShingle | Shicai Liang Huan Wang Hiroto Yamashita Shuning Zhang Xuxu Lang Jiaxuan Yue Shan He Yu Wang Kai Fan Zhaotang Ding Takashi Ikka Wenjun Qian Genome-wide identification and expression analysis of sucrose phosphate synthase and sucrose-6-phosphate phosphatase family genes in Camellia sinensis Beverage Plant Research camellia sinensis spp sps tissue-specific analysis spatio-temporal analysis |
| title | Genome-wide identification and expression analysis of sucrose phosphate synthase and sucrose-6-phosphate phosphatase family genes in Camellia sinensis |
| title_full | Genome-wide identification and expression analysis of sucrose phosphate synthase and sucrose-6-phosphate phosphatase family genes in Camellia sinensis |
| title_fullStr | Genome-wide identification and expression analysis of sucrose phosphate synthase and sucrose-6-phosphate phosphatase family genes in Camellia sinensis |
| title_full_unstemmed | Genome-wide identification and expression analysis of sucrose phosphate synthase and sucrose-6-phosphate phosphatase family genes in Camellia sinensis |
| title_short | Genome-wide identification and expression analysis of sucrose phosphate synthase and sucrose-6-phosphate phosphatase family genes in Camellia sinensis |
| title_sort | genome wide identification and expression analysis of sucrose phosphate synthase and sucrose 6 phosphate phosphatase family genes in camellia sinensis |
| topic | camellia sinensis spp sps tissue-specific analysis spatio-temporal analysis |
| url | https://www.maxapress.com/article/doi/10.48130/bpr-0024-0007 |
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