Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols
Abstract The search has been ongoing for safe and effective antimicrobial agents for control and prevention of oral biofilm associated with disease. Clinical trials for oral specific anti-bacterials are costly and often provide inconclusive results. The simple approach of ex vivo testing of these ag...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-87382-0 |
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| author | Guy R. Adami Wei Li Stefan J. Green Elissa M. Kim Christine D. Wu |
| author_facet | Guy R. Adami Wei Li Stefan J. Green Elissa M. Kim Christine D. Wu |
| author_sort | Guy R. Adami |
| collection | DOAJ |
| description | Abstract The search has been ongoing for safe and effective antimicrobial agents for control and prevention of oral biofilm associated with disease. Clinical trials for oral specific anti-bacterials are costly and often provide inconclusive results. The simple approach of ex vivo testing of these agents has not demonstrated utility, likely due to variability of effects observed even with a single donor. We show how shed oral biofilms, easily obtained from donor saliva, and tested under optimized conditions, respond reproducibly to anti-bacterial challenges measured by reductions in rRNA accumulation in susceptible taxa. Responses are in part donor specific, but many bacteria taxa were shown to be reproducibly susceptible over a group of donors. For two antibiotics, vancomycin and penicillin G tested at pharmacologic levels, a subset of Gram-positive bacteria was inhibited. A natural product with antibacterial properties, diluted Vaccinium macrocarpon (cranberry) juice, was shown to inhibit a range of oral taxa, including Alloprevotella sp__HMT_473, Granulicatella adiacens, Lachnoanaerobaculum umeaense, Lepotrichia sp__HMT_215, Peptostreptococcus stomatis, Prevotella nanceiensis, Stomatobaculum sp__HMT_097, Veillonella parvula, and kill some targets. The model discussed in this study has promise as a rapid, precise, and reproducible ex vivo method to test and identify potential clinically useful antimicrobial agents active against the oral biofilm community. |
| format | Article |
| id | doaj-art-acb0967f9c944d9a98a554ff89215f3f |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-acb0967f9c944d9a98a554ff89215f3f2025-08-20T03:11:07ZengNature PortfolioScientific Reports2045-23222025-02-0115111310.1038/s41598-025-87382-0Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenolsGuy R. Adami0Wei Li1Stefan J. Green2Elissa M. Kim3Christine D. Wu4Department of Oral Medicine and Diagnostics, College of Dentistry, University of Illinois ChicagoDepartment of Pediatric Dentistry, College of Dentistry, University of Illinois ChicagoGenomics and Microbiome Core Facility, Rush UniversityDepartment of Oral Medicine and Diagnostics, College of Dentistry, University of Illinois ChicagoDepartment of Pediatric Dentistry, College of Dentistry, University of Illinois ChicagoAbstract The search has been ongoing for safe and effective antimicrobial agents for control and prevention of oral biofilm associated with disease. Clinical trials for oral specific anti-bacterials are costly and often provide inconclusive results. The simple approach of ex vivo testing of these agents has not demonstrated utility, likely due to variability of effects observed even with a single donor. We show how shed oral biofilms, easily obtained from donor saliva, and tested under optimized conditions, respond reproducibly to anti-bacterial challenges measured by reductions in rRNA accumulation in susceptible taxa. Responses are in part donor specific, but many bacteria taxa were shown to be reproducibly susceptible over a group of donors. For two antibiotics, vancomycin and penicillin G tested at pharmacologic levels, a subset of Gram-positive bacteria was inhibited. A natural product with antibacterial properties, diluted Vaccinium macrocarpon (cranberry) juice, was shown to inhibit a range of oral taxa, including Alloprevotella sp__HMT_473, Granulicatella adiacens, Lachnoanaerobaculum umeaense, Lepotrichia sp__HMT_215, Peptostreptococcus stomatis, Prevotella nanceiensis, Stomatobaculum sp__HMT_097, Veillonella parvula, and kill some targets. The model discussed in this study has promise as a rapid, precise, and reproducible ex vivo method to test and identify potential clinically useful antimicrobial agents active against the oral biofilm community.https://doi.org/10.1038/s41598-025-87382-0 |
| spellingShingle | Guy R. Adami Wei Li Stefan J. Green Elissa M. Kim Christine D. Wu Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols Scientific Reports |
| title | Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols |
| title_full | Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols |
| title_fullStr | Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols |
| title_full_unstemmed | Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols |
| title_short | Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols |
| title_sort | ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols |
| url | https://doi.org/10.1038/s41598-025-87382-0 |
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