Coincidence detection between apical and basal dendrites drives STDP in cerebellar Golgi cells

Abstract Cerebellar Golgi cells (GoCs), segregate parallel fiber (pf), and mossy fiber (mf) inputs on apical and basal dendrites. Computational modeling predicted that this anatomical arrangement, coupled with a specific ionic channel localization, could be instrumental to drive STDP at mf-GoC synap...

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Main Authors: Eleonora Pali, Stefano Masoli, Danila Di Domenico, Teresa Sorbo, Francesca Prestori, Egidio D’Angelo
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-08153-1
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author Eleonora Pali
Stefano Masoli
Danila Di Domenico
Teresa Sorbo
Francesca Prestori
Egidio D’Angelo
author_facet Eleonora Pali
Stefano Masoli
Danila Di Domenico
Teresa Sorbo
Francesca Prestori
Egidio D’Angelo
author_sort Eleonora Pali
collection DOAJ
description Abstract Cerebellar Golgi cells (GoCs), segregate parallel fiber (pf), and mossy fiber (mf) inputs on apical and basal dendrites. Computational modeling predicted that this anatomical arrangement, coupled with a specific ionic channel localization, could be instrumental to drive STDP at mf-GoC synapses. Here, we test this hypothesis with GoC patch-clamp recordings in acute mouse cerebellar slices. Repeated mf-pf pairing on the theta-band within a ± 50 ms time window induces anti-symmetric Hebbian-STDP, with spike-timing long-term potentiation or depression (st-LTP or st-LTD) occurring when action potentials (APs) elicited by pf stimulation follow or precede the activation of mf synapses, respectively. Mf-GoC STDP induction requires AP backpropagation from apical to basal dendrites, NMDA receptor activation at mf-GoC synapses, and intracellular calcium changes. Importantly, STDP is inverted by inhibitory control. Thus, experimental evidence confirms and extends model predictions suggesting that GoC STDP can bind molecular layer to granular layer activity, regulating cerebellar computation and learning.
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spelling doaj-art-aca8233f0def44b6aae8feaf26769c0f2025-08-20T02:25:16ZengNature PortfolioCommunications Biology2399-36422025-05-018111610.1038/s42003-025-08153-1Coincidence detection between apical and basal dendrites drives STDP in cerebellar Golgi cellsEleonora Pali0Stefano Masoli1Danila Di Domenico2Teresa Sorbo3Francesca Prestori4Egidio D’Angelo5Department of Brain and Behavioural Sciences, University of PaviaDepartment of Brain and Behavioural Sciences, University of PaviaDepartment of Brain and Behavioural Sciences, University of PaviaDepartment of Brain and Behavioural Sciences, University of PaviaDepartment of Brain and Behavioural Sciences, University of PaviaDepartment of Brain and Behavioural Sciences, University of PaviaAbstract Cerebellar Golgi cells (GoCs), segregate parallel fiber (pf), and mossy fiber (mf) inputs on apical and basal dendrites. Computational modeling predicted that this anatomical arrangement, coupled with a specific ionic channel localization, could be instrumental to drive STDP at mf-GoC synapses. Here, we test this hypothesis with GoC patch-clamp recordings in acute mouse cerebellar slices. Repeated mf-pf pairing on the theta-band within a ± 50 ms time window induces anti-symmetric Hebbian-STDP, with spike-timing long-term potentiation or depression (st-LTP or st-LTD) occurring when action potentials (APs) elicited by pf stimulation follow or precede the activation of mf synapses, respectively. Mf-GoC STDP induction requires AP backpropagation from apical to basal dendrites, NMDA receptor activation at mf-GoC synapses, and intracellular calcium changes. Importantly, STDP is inverted by inhibitory control. Thus, experimental evidence confirms and extends model predictions suggesting that GoC STDP can bind molecular layer to granular layer activity, regulating cerebellar computation and learning.https://doi.org/10.1038/s42003-025-08153-1
spellingShingle Eleonora Pali
Stefano Masoli
Danila Di Domenico
Teresa Sorbo
Francesca Prestori
Egidio D’Angelo
Coincidence detection between apical and basal dendrites drives STDP in cerebellar Golgi cells
Communications Biology
title Coincidence detection between apical and basal dendrites drives STDP in cerebellar Golgi cells
title_full Coincidence detection between apical and basal dendrites drives STDP in cerebellar Golgi cells
title_fullStr Coincidence detection between apical and basal dendrites drives STDP in cerebellar Golgi cells
title_full_unstemmed Coincidence detection between apical and basal dendrites drives STDP in cerebellar Golgi cells
title_short Coincidence detection between apical and basal dendrites drives STDP in cerebellar Golgi cells
title_sort coincidence detection between apical and basal dendrites drives stdp in cerebellar golgi cells
url https://doi.org/10.1038/s42003-025-08153-1
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