NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe Sepsis
The aim of this first study was to reveal the new potential biomarkers by a metabolomics approach in severe septic calves. Sepsis is a common cause of morbidity and mortality in newborn dairy calves. The main challenges with the use of biomarkers of sepsis in domestic animals are their availability,...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/8016510 |
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| author | Abdullah Basoglu Ismail Sen Gaia Meoni Leonardo Tenori Amir Naseri |
| author_facet | Abdullah Basoglu Ismail Sen Gaia Meoni Leonardo Tenori Amir Naseri |
| author_sort | Abdullah Basoglu |
| collection | DOAJ |
| description | The aim of this first study was to reveal the new potential biomarkers by a metabolomics approach in severe septic calves. Sepsis is a common cause of morbidity and mortality in newborn dairy calves. The main challenges with the use of biomarkers of sepsis in domestic animals are their availability, cost, and time required to obtain a result. Metabolomics may offer the potential to identify biomarkers that define calf sepsis in terms of combined clinical, physiological, and pathobiological abnormalities. To our knowledge, this is the first study presenting an NMR- (nuclear magnetic resonance-) based plasma metabolomics at set intervals in neonatal septic calves. Twenty neonatal dairy calves with severe sepsis and ten healthy calves were used. Hematological and biochemical health profiles were gathered in plasma samples at set intervals. Similarly, NMR spectra were acquired. All diseased animals (except one) died after 72 hours. Clinical and laboratory results were in accordance with those of severe septic animals. Multivariate analysis on NMR plasma spectra proved to be an excellent tool for faster identification of calves with severe sepsis from healthy animals. The NMR-based metabolomic profile may contribute to the better understanding of severe sepsis in newborn calves. |
| format | Article |
| id | doaj-art-aca0981f8cf544efa297733dd1514e88 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-aca0981f8cf544efa297733dd1514e882025-02-03T01:02:13ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/80165108016510NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe SepsisAbdullah Basoglu0Ismail Sen1Gaia Meoni2Leonardo Tenori3Amir Naseri4Department of Internal Medicine, Faculty of Veterinary Medicine, Selçuk University, Aleaddin Keykubat Campus 42003 Konya, TurkeyDepartment of Internal Medicine, Faculty of Veterinary Medicine, Selçuk University, Aleaddin Keykubat Campus 42003 Konya, TurkeyMagnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, Largo Brambilla 3, 50134 Florence, ItalyDepartment of Internal Medicine, Faculty of Veterinary Medicine, Selçuk University, Aleaddin Keykubat Campus 42003 Konya, TurkeyThe aim of this first study was to reveal the new potential biomarkers by a metabolomics approach in severe septic calves. Sepsis is a common cause of morbidity and mortality in newborn dairy calves. The main challenges with the use of biomarkers of sepsis in domestic animals are their availability, cost, and time required to obtain a result. Metabolomics may offer the potential to identify biomarkers that define calf sepsis in terms of combined clinical, physiological, and pathobiological abnormalities. To our knowledge, this is the first study presenting an NMR- (nuclear magnetic resonance-) based plasma metabolomics at set intervals in neonatal septic calves. Twenty neonatal dairy calves with severe sepsis and ten healthy calves were used. Hematological and biochemical health profiles were gathered in plasma samples at set intervals. Similarly, NMR spectra were acquired. All diseased animals (except one) died after 72 hours. Clinical and laboratory results were in accordance with those of severe septic animals. Multivariate analysis on NMR plasma spectra proved to be an excellent tool for faster identification of calves with severe sepsis from healthy animals. The NMR-based metabolomic profile may contribute to the better understanding of severe sepsis in newborn calves.http://dx.doi.org/10.1155/2018/8016510 |
| spellingShingle | Abdullah Basoglu Ismail Sen Gaia Meoni Leonardo Tenori Amir Naseri NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe Sepsis Mediators of Inflammation |
| title | NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe Sepsis |
| title_full | NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe Sepsis |
| title_fullStr | NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe Sepsis |
| title_full_unstemmed | NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe Sepsis |
| title_short | NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe Sepsis |
| title_sort | nmr based plasma metabolomics at set intervals in newborn dairy calves with severe sepsis |
| url | http://dx.doi.org/10.1155/2018/8016510 |
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